Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AO353_RS08710 Genome accession   NZ_CP012830
Coordinates   1913199..1914980 (+) Length   593 a.a.
NCBI ID   WP_054594559.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1908199..1919980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS08690 (AO353_08690) argE 1909265..1910413 (-) 1149 WP_054594555.1 acetylornithine deacetylase -
  AO353_RS08695 (AO353_08695) - 1910572..1911939 (+) 1368 WP_054594556.1 inorganic triphosphatase -
  AO353_RS08700 (AO353_08700) - 1912125..1912595 (-) 471 WP_054594557.1 Lrp/AsnC family transcriptional regulator -
  AO353_RS08705 (AO353_08705) - 1912721..1913059 (+) 339 WP_054594558.1 hypothetical protein -
  AO353_RS08710 (AO353_08710) pilB 1913199..1914980 (+) 1782 WP_054594559.1 GspE/PulE family protein Machinery gene
  AO353_RS08715 (AO353_08715) - 1915078..1915422 (+) 345 WP_054594560.1 DUF2388 domain-containing protein -
  AO353_RS08720 (AO353_08720) gcvP 1915686..1918559 (-) 2874 WP_054594561.1 aminomethyl-transferring glycine dehydrogenase -
  AO353_RS08725 (AO353_08725) - 1918714..1919853 (+) 1140 WP_054594562.1 DegT/DnrJ/EryC1/StrS family aminotransferase -

Sequence

Protein


Download         Length: 593 a.a.        Molecular weight: 66269.95 Da        Isoelectric Point: 6.6932

>NTDB_id=136609 AO353_RS08710 WP_054594559.1 1913199..1914980(+) (pilB) [Pseudomonas fluorescens strain FW300-N2E3]
MPVQLATQDRWLDLNDLLRELVAQGFISQDSAEHALTTRRNASNSQLHPLEFLASQHLDDLSRPGKRLDLESLTLWLSQQ
AGQPYLRIDPLKINVAQITSLMSYAFAQRHKILAVAIDRDAVTVASAQPYVRSWEADLTHVLQLPIKRVVANPADIQRFS
VEFFRLAKSVSGATNADQQMNTLGNFEQLLNLGASDQEPDANDAHIVNIVDWLFQYAFQQRASDIHIEPRREQGTVRFRI
DGVLHNVYQFPPQVTMAIVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGGEVELRLSTLPTAFGEKMVMRIFDPEVLLKDF
DQLGFSADDLRRWQDMTRQPNGIILVTGPTGSGKTSTLYTTLKKLATPEVNLCTIEDPIEMVEPAFNQMQVQHNIDLTFA
SGVRALMRQDPDIIMIGEIRDLETAEMAIQAALTGHLVLSTLHTNDAPSAISRLLELGVPHYLIKATLLGVMAQRLVRTL
CPHCKAPLTLGEDDWQTLTRPWQAPLPGNAQRAIGCLECRDTGYRGRAGVYEIMQMTDSVKALINPDTDLLAVRRQAFKE
GMRSLRLSGAQKVAAGLTTIEEVLRVTPQSEQK

Nucleotide


Download         Length: 1782 bp        

>NTDB_id=136609 AO353_RS08710 WP_054594559.1 1913199..1914980(+) (pilB) [Pseudomonas fluorescens strain FW300-N2E3]
ATGCCCGTTCAACTCGCCACTCAGGATCGCTGGCTGGATCTCAATGACCTGCTGCGCGAACTGGTCGCCCAAGGCTTCAT
CAGCCAGGACTCGGCCGAACACGCGCTGACCACTCGCCGCAATGCCAGCAACAGCCAGTTGCACCCGCTGGAGTTCCTTG
CCAGCCAACACCTCGACGACCTCAGCCGCCCCGGTAAACGCCTGGATCTGGAAAGCCTGACCCTGTGGCTCTCGCAGCAG
GCCGGCCAGCCTTACTTGCGCATCGACCCGCTGAAAATAAACGTCGCGCAAATTACCTCGCTGATGTCTTACGCCTTTGC
CCAGCGCCACAAGATCCTCGCGGTAGCGATCGACCGCGACGCCGTCACTGTCGCTAGCGCGCAGCCTTATGTGCGCAGCT
GGGAAGCCGACCTGACCCACGTTCTACAGTTGCCGATCAAACGGGTAGTGGCCAACCCGGCGGATATCCAGCGCTTCAGC
GTGGAATTCTTCCGCCTGGCCAAATCGGTCAGCGGCGCGACCAATGCCGATCAGCAAATGAACACCCTGGGCAACTTCGA
ACAGTTGCTCAACCTCGGCGCCAGCGATCAGGAACCGGACGCCAACGACGCGCACATCGTCAACATCGTCGACTGGTTGT
TCCAGTACGCTTTCCAGCAACGCGCCAGTGATATCCACATCGAACCACGGCGCGAGCAAGGCACCGTGCGCTTTCGCATC
GACGGCGTATTGCACAACGTCTATCAATTTCCACCGCAGGTGACCATGGCGATCGTCAGTCGCCTGAAGAGCCTGGGGCG
GATGAACGTTGCAGAAAAGCGCAAACCCCAGGATGGCCGGGTCAAGACCAAGACTCCGGACGGCGGCGAAGTAGAATTGC
GGCTGTCGACGCTACCCACGGCATTCGGGGAAAAAATGGTCATGCGGATCTTCGATCCGGAAGTGCTGCTCAAGGACTTC
GACCAGCTCGGGTTTTCCGCCGATGATCTGCGCCGCTGGCAGGACATGACCCGCCAGCCCAACGGCATCATTCTGGTCAC
CGGGCCGACCGGTTCGGGCAAGACCAGCACGCTCTACACCACACTGAAAAAACTCGCGACGCCAGAGGTCAACCTCTGCA
CCATCGAAGACCCGATCGAAATGGTCGAGCCGGCGTTCAATCAGATGCAGGTCCAGCACAACATCGACCTGACCTTTGCC
AGCGGTGTGCGCGCACTGATGCGACAGGATCCGGACATCATCATGATCGGCGAGATCCGCGACCTCGAGACCGCCGAAAT
GGCCATTCAAGCGGCACTCACCGGGCACCTGGTGTTGTCGACCCTGCACACCAACGACGCACCGAGCGCCATCAGCCGCT
TGCTGGAACTCGGCGTGCCGCATTACCTGATCAAAGCCACCCTGCTGGGAGTCATGGCGCAACGTCTGGTCCGCACGCTG
TGCCCGCATTGTAAGGCACCGCTGACGCTGGGCGAAGACGACTGGCAAACCTTGACCCGTCCCTGGCAAGCACCGCTGCC
AGGCAACGCCCAACGCGCGATTGGCTGCCTGGAGTGCCGCGACACCGGTTATCGCGGACGTGCCGGGGTCTACGAAATCA
TGCAAATGACCGACAGTGTCAAAGCCCTGATCAACCCCGACACCGACCTGCTCGCCGTCCGTCGCCAGGCGTTCAAGGAA
GGCATGCGCAGCCTGCGGCTGTCGGGTGCGCAAAAAGTGGCGGCGGGGCTGACGACGATTGAAGAGGTGCTGCGAGTGAC
GCCGCAGAGCGAGCAGAAATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(21-164)

Type II/IV secretion system protein (T2SSE)

(215-479)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.896

97.639

0.39