Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AO055_RS21015 Genome accession   NZ_CP012802
Coordinates   4061038..4061505 (+) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O157:H7 strain WS4202     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4056038..4066505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO055_RS20965 (AO055_20940) rplP 4056148..4056558 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  AO055_RS20970 (AO055_20945) rpsC 4056571..4057272 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  AO055_RS20975 (AO055_20950) rplV 4057290..4057622 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  AO055_RS20980 (AO055_20955) rpsS 4057637..4057915 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  AO055_RS20985 (AO055_20960) rplB 4057932..4058753 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  AO055_RS20990 (AO055_20965) rplW 4058771..4059073 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  AO055_RS20995 (AO055_20970) rplD 4059070..4059675 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  AO055_RS21000 (AO055_20975) rplC 4059686..4060315 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  AO055_RS21005 (AO055_20980) rpsJ 4060348..4060659 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  AO055_RS21015 (AO055_20990) pilD 4061038..4061505 (+) 468 WP_000381746.1 prepilin peptidase Machinery gene
  AO055_RS21020 (AO055_20995) bfr 4061502..4061978 (-) 477 WP_000675516.1 bacterioferritin -
  AO055_RS21025 (AO055_21000) bfd 4062051..4062245 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  AO055_RS21030 (AO055_21005) tuf 4062428..4063612 (-) 1185 WP_000031783.1 elongation factor Tu -
  AO055_RS21035 (AO055_21010) fusA 4063683..4065797 (-) 2115 WP_000124700.1 elongation factor G -
  AO055_RS21040 (AO055_21015) rpsG 4065894..4066364 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=136433 AO055_RS21015 WP_000381746.1 4061038..4061505(+) (pilD) [Escherichia coli O157:H7 strain WS4202]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=136433 AO055_RS21015 WP_000381746.1 4061038..4061505(+) (pilD) [Escherichia coli O157:H7 strain WS4202]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCCTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387

Multiple sequence alignment