Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   GBS85147_RS04245 Genome accession   NZ_CP010319
Coordinates   816190..817047 (+) Length   285 a.a.
NCBI ID   WP_000163016.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GBS85147     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 811190..822047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBS85147_RS04215 (GBS85147_0853) - 811506..812387 (+) 882 WP_053039137.1 F0F1 ATP synthase subunit gamma -
  GBS85147_RS04220 (GBS85147_0854) atpD 812461..813867 (+) 1407 WP_000094375.1 F0F1 ATP synthase subunit beta -
  GBS85147_RS04225 (GBS85147_0855) - 813880..814293 (+) 414 WP_000068053.1 F0F1 ATP synthase subunit epsilon -
  GBS85147_RS04230 (GBS85147_0856) - 814358..814588 (+) 231 WP_000602601.1 DUF1146 family protein -
  GBS85147_RS04235 (GBS85147_0857) murA 814651..815922 (+) 1272 WP_000357877.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  GBS85147_RS04240 (GBS85147_0858) - 815924..816115 (+) 192 WP_000168511.1 DNA-directed RNA polymerase subunit beta -
  GBS85147_RS04245 (GBS85147_0859) endA 816190..817047 (+) 858 WP_000163016.1 DNA/RNA non-specific endonuclease Machinery gene
  GBS85147_RS04250 (GBS85147_0860) pheS 817338..818378 (+) 1041 WP_000365655.1 phenylalanine--tRNA ligase subunit alpha -
  GBS85147_RS04255 (GBS85147_0861) - 818461..818982 (+) 522 WP_000078401.1 GNAT family N-acetyltransferase -
  GBS85147_RS04260 (GBS85147_0862) pheT 819036..821441 (+) 2406 WP_000961581.1 phenylalanine--tRNA ligase subunit beta -

Sequence

Protein


Download         Length: 285 a.a.        Molecular weight: 31398.38 Da        Isoelectric Point: 10.5024

>NTDB_id=136373 GBS85147_RS04245 WP_000163016.1 816190..817047(+) (endA) [Streptococcus agalactiae strain GBS85147]
MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHFLKQVTDLIISNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGT
NIAWNQSGAFIINQNKTDLNAKVSSAPYAINEIKRVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYETLVRKALDRHKAVRYRVTLIYDR
DNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDYATGKVKQTK

Nucleotide


Download         Length: 858 bp        

>NTDB_id=136373 GBS85147_RS04245 WP_000163016.1 816190..817047(+) (endA) [Streptococcus agalactiae strain GBS85147]
ATGACAAAAAAACAAAAACAAATAACAGCTTCGATTGTAACTTTGCTTTTTTCATTACTGGCAATAGGGTTAGGTGAAAG
CAAATCTTTGCCTAATGATCACTTTTTAAAACAGGTGACAGATTTAATTATTAGTAATGAATCTGGCAAAAATACTTCCC
TTAAAAATATATCAGGAACCCCTAGTAGAGAGTTATCTGAATCTGTTTTGACTAGCAATGTTAAAAAACAATTGGGAACA
AATATAGCTTGGAACCAGTCAGGTGCTTTTATCATTAATCAAAATAAAACAGATTTGAATGCTAAAGTGTCAAGTGCACC
ATATGCTATTAATGAAATAAAGAGGGTTAATAACCAAATTGTTCCAACTAAAGCAAATGCCTTATTAACAAAAGCAACTC
GTCAATATCGAAATCGAGAAGAAACAGGGAATGGTAGAACTTATTGGAAACCAGCTGGATGGCATCAGATAAATGGATTA
AAAGGGAGCTATAACCACGCTGTGGACAGAGGCCATTTAATTGGTTATGCTTTAGTAGGGAGTTTAAGAGGATTTGATGC
CTCCACAAGTAATCCTAAAAATATTGCCACACAAGCAGCTTGGGCAAACCAAGCTAATAGCAACCAATCAACAGGGCAAA
ATTATTATGAAACTTTAGTTCGTAAAGCATTGGATCGTCATAAAGCTGTTAGGTATCGTGTGACTTTAATTTATGACAGA
GATAATCTTTTATCTTCGGGATCACATATTGAGGCTAAATCTTCAGATGGAAGTTTAGAATTTAATGTTTTTATTCCTAA
TGTACAATCGGGATTATTATTTGATTATGCAACAGGAAAAGTGAAGCAGACAAAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(87-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

66.379

81.404

0.54
  endA Streptococcus pneumoniae D39

66.379

81.404

0.54
  endA Streptococcus pneumoniae R6

66.379

81.404

0.54
  endA Streptococcus pneumoniae TIGR4

66.379

81.404

0.54

Multiple sequence alignment