Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   RA0C_RS05130 Genome accession   NC_017045
Coordinates   1064649..1065752 (-) Length   367 a.a.
NCBI ID   WP_004918085.1    Uniprot ID   -
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Inactivation of dprA abrogated natural transformation in RA ATCC11845, and this effect was restored by the expression of dprA in trans.


Genomic Context

Location: 1059649..1070752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS10465 (RA0C_1067) - 1060616..1060756 (+) 141 WP_015345427.1 hypothetical protein -
  RA0C_RS05105 (RA0C_1068) comF 1060844..1061497 (-) 654 WP_004918078.1 ComF family protein Machinery gene
  RA0C_RS05115 (RA0C_1070) - 1061719..1062270 (-) 552 WP_004918080.1 hypothetical protein -
  RA0C_RS05120 (RA0C_1071) - 1062325..1062975 (-) 651 WP_004918082.1 HD domain-containing protein -
  RA0C_RS05125 (RA0C_1072) gdhA 1063107..1064465 (-) 1359 WP_014938006.1 NADP-specific glutamate dehydrogenase -
  RA0C_RS05130 (RA0C_1073) dprA 1064649..1065752 (-) 1104 WP_004918085.1 DNA-processing protein DprA Machinery gene
  RA0C_RS05135 (RA0C_1074) - 1065755..1066132 (-) 378 WP_004918086.1 group III truncated hemoglobin -
  RA0C_RS05140 (RA0C_1075) ruvC 1066143..1066697 (-) 555 WP_004918088.1 crossover junction endodeoxyribonuclease RuvC -
  RA0C_RS05145 (RA0C_1076) - 1066721..1067911 (-) 1191 WP_004918090.1 carboxypeptidase-like regulatory domain-containing protein -
  RA0C_RS05150 (RA0C_1077) - 1067941..1068414 (-) 474 WP_004918092.1 Lrp/AsnC family transcriptional regulator -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40491.51 Da        Isoelectric Point: 6.6968

>NTDB_id=1342 RA0C_RS05130 WP_004918085.1 1064649..1065752(-) (dprA) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MVNAEEILYSIALRRSPLVGDVIFASLVSEVGSAKEAWGLSKLELRKISGVGKQIASNIGDKNLLKFAEKEIEFCIKNEI
KIHLRHLNQLPRLLGECYDAPAILFQKGDYEDTANNISIVGTRNATAYGKSFLEDLIVQFKNKNIQVISGLALGTDGIAH
QEALKNNIKTSAVLAHGLHIIYPSKHKILAEEILNNGGALFSEFCSDEKPDREHFLQRNRIVAGLSANTIVVESAYAGGS
ISTASFANQYNREVYALAGRLTDKYSQGCNHLIFQNKAKIISSIKGLLKDLDLDNTSKVLELFPSVEVSLTPVQEPVYDI
IKEVNDISLDDLSEKLNQPISKVFPVLLELELLGLIRANSGRRYSII

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=1342 RA0C_RS05130 WP_004918085.1 1064649..1065752(-) (dprA) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGGTAAATGCGGAAGAAATTTTATATTCTATTGCTCTTCGGCGAAGCCCATTGGTGGGAGATGTTATATTTGCAAGTTT
GGTTAGCGAGGTAGGCTCTGCTAAGGAAGCGTGGGGTCTGTCTAAGTTGGAATTACGGAAAATATCAGGCGTAGGTAAGC
AAATTGCCTCAAACATCGGAGATAAAAATCTTTTAAAATTTGCTGAAAAGGAAATAGAGTTCTGCATTAAAAACGAAATA
AAAATCCACTTACGACATCTTAATCAGCTTCCTCGTCTTTTAGGTGAATGTTATGATGCTCCTGCTATTCTTTTTCAAAA
AGGAGATTATGAGGATACCGCTAATAATATTAGTATTGTTGGGACGAGAAATGCCACTGCTTATGGTAAGTCGTTTTTGG
AGGATTTAATTGTTCAGTTTAAAAATAAAAATATTCAAGTCATTAGTGGTTTAGCTTTAGGTACAGACGGAATAGCTCAT
CAAGAGGCTTTAAAAAACAATATAAAAACTTCAGCGGTTTTAGCACACGGTTTACATATCATTTATCCGTCCAAACATAA
AATTCTCGCTGAAGAAATATTAAATAATGGTGGAGCTTTGTTTTCTGAATTCTGCTCTGATGAAAAGCCAGATAGAGAAC
ATTTTTTGCAAAGAAATCGTATTGTGGCAGGGCTTTCGGCTAATACGATTGTGGTAGAGTCTGCTTATGCAGGAGGCTCT
ATTAGCACGGCGAGTTTTGCTAATCAGTATAATAGAGAAGTGTATGCTTTGGCAGGGCGTCTTACAGATAAATATAGCCA
AGGTTGCAATCATCTTATTTTTCAAAATAAAGCTAAAATCATATCTTCTATTAAAGGTTTGCTAAAAGACCTAGATTTGG
ATAATACTTCTAAAGTTTTGGAGCTTTTTCCTTCAGTTGAAGTTTCTTTAACTCCTGTTCAGGAGCCTGTTTATGATATT
ATTAAGGAGGTTAATGATATTTCATTAGATGACTTGTCAGAAAAATTAAATCAACCCATATCTAAAGTGTTTCCTGTTTT
ATTGGAATTAGAATTATTGGGGTTGATAAGGGCAAATTCTGGGAGGAGATATTCTATCATTTAG

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(77-281)

DprA winged helix domain (DprA_WH)

(309-361)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]
[2] Li Huang et al. (2019) DprA Is Essential for Natural Competence in Riemerella anatipestifer and Has a Conserved Evolutionary Mechanism. Frontiers in Genetics 10:429. [PMID: 31156696]