Detailed information    

experimental Experimentally validated

Overview

Name   tonBA   Type   Unclear
Locus tag   RA0C_RS05665 Genome accession   NC_017045
Coordinates   1173261..1174121 (+) Length   286 a.a.
NCBI ID   WP_004917253.1    Uniprot ID   E4TC47
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

Mutation of tonBA or tonBB, but not tbfA, was shown to inhibit the natural transformation of R. anatipestifer ATCC 11845 in the GCB medium. In parallel, it was shown that the tonBB mutant, but not the tonBA mutant, decreased iron acquisition in the GCB medium. This result suggested that the tonBB mutant affects the natural transformation frequency due to the deficiency of iron utilization.


Genomic Context

Location: 1168261..1179121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS05645 (RA0C_1179) - 1168773..1171448 (+) 2676 WP_004917270.1 carboxypeptidase regulatory-like domain-containing protein -
  RA0C_RS05650 (RA0C_1180) - 1171608..1172063 (+) 456 WP_004917268.1 hypothetical protein -
  RA0C_RS05655 (RA0C_1191) - 1172161..1172841 (+) 681 WP_013446946.1 MotA/TolQ/ExbB proton channel family protein -
  RA0C_RS05660 (RA0C_1192) - 1172858..1173256 (+) 399 WP_004917263.1 ExbD/TolR family protein -
  RA0C_RS05665 (RA0C_1193) tonBA 1173261..1174121 (+) 861 WP_004917253.1 hypothetical protein Unclear
  RA0C_RS05670 (RA0C_1194) - 1174165..1175373 (+) 1209 WP_004917252.1 NAD(P)/FAD-dependent oxidoreductase -
  RA0C_RS05675 (RA0C_1195) ccsA 1175436..1176230 (-) 795 WP_004917251.1 cytochrome c biogenesis protein CcsA -
  RA0C_RS05680 (RA0C_1196) - 1176233..1177459 (-) 1227 WP_004917249.1 cytochrome c biogenesis protein ResB -
  RA0C_RS05685 (RA0C_1197) nrfA 1177537..1179003 (-) 1467 WP_004917246.1 ammonia-forming cytochrome c nitrite reductase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 30606.19 Da        Isoelectric Point: 9.6762

>NTDB_id=1341 RA0C_RS05665 WP_004917253.1 1173261..1174121(+) (tonBA) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MSQTINTSERKDQIKSAIITFLISLLVFLALYFYSFTRELPKEEVVSTMLINFGDQNEGNLPEEPQNQEGSLSATEAPTP
IQEIQPTPVEPQPKKEVVKEKIITGQNTKTSAEKVEKVTSKPTKESTANTQKKETTTPKKSSTTTQNKTVTNQGDGRGNE
AIGNLIRGRGANKGAQGNSQNTSGNSGDPLGGDSNGDSKIGIDRKLIGFIPGTMGRGGSQPTHQCSASGTISISYVVDKA
GNVISARRSGGITDPCAVNTTIEWVKKYVKAERASTSSTGTYKITF

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1341 RA0C_RS05665 WP_004917253.1 1173261..1174121(+) (tonBA) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGAGCCAAACCATAAATACATCGGAAAGAAAAGACCAAATCAAAAGTGCCATCATCACATTTTTGATTAGCCTTTTGGT
ATTCTTAGCTTTGTATTTTTATTCGTTTACTAGAGAACTTCCAAAGGAGGAAGTCGTAAGCACGATGCTCATCAATTTTG
GAGACCAAAACGAGGGTAACCTGCCTGAAGAACCCCAAAACCAAGAGGGCAGTCTATCCGCCACCGAAGCACCTACACCA
ATACAAGAAATACAACCTACACCTGTAGAACCTCAGCCCAAAAAAGAAGTGGTAAAGGAGAAAATTATCACAGGGCAAAA
TACCAAAACAAGTGCTGAAAAGGTGGAAAAAGTAACCTCTAAACCTACAAAAGAAAGCACAGCAAACACCCAAAAGAAAG
AAACCACGACACCTAAAAAAAGCAGTACCACTACACAAAATAAAACCGTGACCAACCAAGGTGATGGCAGAGGTAACGAG
GCTATTGGCAACCTCATCAGAGGGCGAGGTGCCAACAAAGGAGCTCAAGGAAACTCCCAAAACACTTCCGGCAATTCAGG
AGACCCACTAGGTGGCGATAGCAATGGCGATAGTAAAATCGGCATAGACCGAAAGTTAATCGGATTTATACCTGGAACTA
TGGGGCGTGGTGGTTCACAGCCGACCCACCAGTGTTCTGCCTCAGGCACCATTAGCATCTCTTATGTGGTAGATAAAGCG
GGCAATGTAATTTCGGCTAGACGAAGTGGCGGTATTACAGACCCTTGTGCCGTAAACACAACCATAGAGTGGGTAAAGAA
ATATGTAAAAGCAGAAAGAGCTAGCACTTCTTCTACAGGCACTTACAAAATCACTTTCTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TC47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Zhang et al. (2021) Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer. Frontiers in Microbiology 12:644868. [PMID: 34447355]