Detailed information    

experimental Experimentally validated

Overview

Name   tonBB   Type   Unclear
Locus tag   RA0C_RS05740 Genome accession   NC_017045
Coordinates   1190258..1191103 (-) Length   281 a.a.
NCBI ID   WP_004917233.1    Uniprot ID   S5WD13
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

Mutation of tonBA or tonBB, but not tbfA, was shown to inhibit the natural transformation of R. anatipestifer ATCC 11845 in the GCB medium. In parallel, it was shown that the tonBB mutant, but not the tonBA mutant, decreased iron acquisition in the GCB medium. This result suggested that the tonBB mutant affects the natural transformation frequency due to the deficiency of iron utilization.


Genomic Context

Location: 1185258..1196103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS05710 (RA0C_1203) - 1186066..1186404 (+) 339 WP_004917238.1 DUF3276 family protein -
  RA0C_RS05720 (RA0C_1205) bshB1 1186575..1187294 (-) 720 WP_004917237.1 bacillithiol biosynthesis deacetylase BshB1 -
  RA0C_RS05725 (RA0C_1206) - 1187392..1189050 (-) 1659 WP_049770578.1 tetratricopeptide repeat protein -
  RA0C_RS05730 (RA0C_1207) - 1189138..1190007 (-) 870 WP_004917235.1 PstS family phosphate ABC transporter substrate-binding protein -
  RA0C_RS05735 (RA0C_1208) - 1190000..1190194 (-) 195 WP_004917234.1 hypothetical protein -
  RA0C_RS05740 (RA0C_1209) tonBB 1190258..1191103 (-) 846 WP_004917233.1 energy transducer TonB Unclear
  RA0C_RS05745 (RA0C_1210) - 1191132..1191680 (-) 549 WP_004917230.1 ExbD/TolR family protein -
  RA0C_RS05750 (RA0C_1211) - 1191690..1192292 (-) 603 WP_004917229.1 ExbD/TolR family protein -
  RA0C_RS05755 (RA0C_1212) - 1192395..1193261 (-) 867 WP_004917228.1 MotA/TolQ/ExbB proton channel family protein -

Sequence

Protein


Download         Length: 281 a.a.        Molecular weight: 30957.12 Da        Isoelectric Point: 4.6112

>NTDB_id=1340 RA0C_RS05740 WP_004917233.1 1190258..1191103(-) (tonBB) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MSDENLGYNPSLDEIVFENRNKAYGAYDLRQSYKSILTRSMIIGTTLFCLAAATPFIVMKIKQLTAPPKQEVEANLMEIL
PEEEPILEQPKEETPPPPPPPKVEEEKIEIIQNVVPEPVKAPTVETPPPPISKQLETTTGLVNQEGVKKPSYAPPPPPPS
TGKGTTVEVKPQVSTTEVYTTVDQEAEFSGGGINGFRSAFQESFDTSVMEGDEGTLKAEVTFVVERDGSLSQVKVTGSNS
TFNREAERAVKSIKKKWTPGKVNGEPVRSRFRMPVAMNFEY

Nucleotide


Download         Length: 846 bp        

>NTDB_id=1340 RA0C_RS05740 WP_004917233.1 1190258..1191103(-) (tonBB) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGTCAGATGAAAATTTAGGGTACAATCCAAGTTTGGATGAGATTGTATTTGAAAACAGAAATAAAGCCTATGGTGCTTA
TGACCTAAGACAGAGCTATAAGAGTATTCTTACAAGGTCTATGATTATAGGTACTACTTTGTTCTGTTTAGCTGCGGCTA
CTCCGTTTATTGTTATGAAAATTAAACAGCTAACGGCACCGCCTAAACAAGAAGTAGAGGCTAATCTTATGGAGATTCTT
CCAGAGGAAGAACCTATTTTGGAGCAACCTAAAGAGGAAACTCCACCGCCACCACCTCCACCAAAAGTGGAAGAGGAGAA
GATTGAAATTATTCAGAATGTGGTTCCAGAACCAGTGAAAGCTCCTACAGTGGAAACCCCACCGCCACCTATATCTAAGC
AGCTAGAAACTACTACAGGTTTGGTAAACCAAGAAGGGGTTAAGAAACCTTCTTATGCACCACCTCCACCTCCTCCTTCT
ACAGGAAAAGGGACGACGGTAGAGGTAAAGCCTCAAGTGAGTACTACTGAAGTGTATACAACGGTAGACCAAGAAGCGGA
GTTCTCAGGCGGAGGTATAAACGGATTTAGGAGTGCTTTCCAAGAGTCTTTTGATACTTCGGTAATGGAAGGTGATGAGG
GTACTTTAAAAGCTGAGGTTACTTTCGTAGTAGAGAGAGATGGCTCACTTTCTCAGGTAAAAGTTACAGGTTCTAACTCT
ACATTTAATAGAGAGGCAGAAAGAGCTGTAAAATCCATCAAAAAGAAATGGACTCCTGGTAAAGTAAATGGAGAGCCAGT
TCGTTCAAGATTTAGAATGCCAGTGGCAATGAATTTTGAGTATTAA

Domains

Predicted by InterproScan.

Gram-negative bacterial TonB protein C-terminal (TonB_C)

(218-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S5WD13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Zhang et al. (2021) Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer. Frontiers in Microbiology 12:644868. [PMID: 34447355]