Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   QI18_RS11675 Genome accession   NZ_CP010050
Coordinates   2325608..2326090 (-) Length   160 a.a.
NCBI ID   WP_039116460.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2320608..2331090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QI18_RS11640 (QI18_11640) - 2320724..2321101 (-) 378 WP_003129983.1 pyridoxamine 5'-phosphate oxidase family protein -
  QI18_RS11645 (QI18_11645) - 2321306..2322172 (+) 867 WP_039116457.1 RluA family pseudouridine synthase -
  QI18_RS11650 (QI18_11650) - 2322211..2323020 (-) 810 WP_014570791.1 metal ABC transporter permease -
  QI18_RS11655 (QI18_11655) - 2323013..2323750 (-) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  QI18_RS11660 (QI18_11660) - 2323927..2324769 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  QI18_RS11665 (QI18_11665) - 2324766..2325203 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  QI18_RS11670 (QI18_11670) comGG 2325285..2325569 (-) 285 WP_025017139.1 competence type IV pilus minor pilin ComGG Machinery gene
  QI18_RS11675 (QI18_11675) comGF 2325608..2326090 (-) 483 WP_039116460.1 competence type IV pilus minor pilin ComGF Machinery gene
  QI18_RS11680 (QI18_11680) comGD 2326062..2326421 (-) 360 WP_023188582.1 competence type IV pilus minor pilin ComGD Machinery gene
  QI18_RS11685 (QI18_11685) comGC 2326453..2326722 (-) 270 WP_023188581.1 competence type IV pilus major pilin ComGC Machinery gene
  QI18_RS11690 (QI18_11690) comGB 2326849..2327922 (-) 1074 WP_080756637.1 competence type IV pilus assembly protein ComGB Machinery gene
  QI18_RS11695 (QI18_11695) comGA 2327816..2328753 (-) 938 Protein_2302 competence type IV pilus ATPase ComGA -

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 18577.40 Da        Isoelectric Point: 8.7953

>NTDB_id=133735 QI18_RS11675 WP_039116460.1 2325608..2326090(-) (comGF) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
MENLKRKSVKAYLLLESLISIALLAFLVSFIVSSLVQVRQKDTEENQKIEALNVAQVAISQSDSIKDWQIFCQQMRFELS
GTKLDRVEQNFLYVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide


Download         Length: 483 bp        

>NTDB_id=133735 QI18_RS11675 WP_039116460.1 2325608..2326090(-) (comGF) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
GTGGAAAATTTAAAAAGAAAATCAGTTAAGGCATATTTGCTTTTGGAAAGTTTAATTAGTATAGCTCTACTCGCTTTTCT
AGTCAGCTTTATCGTAAGTTCTCTTGTGCAAGTAAGACAAAAAGATACGGAAGAAAATCAAAAGATTGAAGCTTTAAACG
TGGCACAAGTGGCGATTAGTCAAAGTGATAGCATAAAAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCA
GGAACAAAATTAGATAGGGTAGAACAGAATTTTCTGTATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGA
TGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTATCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTA
CTAAAAATTTAATAACCATAAGAATTGATTTTAATCAAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACG
TAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(13-60)

Putative Competence protein ComGF (ComGF)

(60-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

59.603

94.375

0.563

Multiple sequence alignment