Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BF28_RS25130 Genome accession   NZ_CP009968
Coordinates   4313494..4317009 (-) Length   1171 a.a.
NCBI ID   WP_000058552.1    Uniprot ID   -
Organism   Bacillus cereus E33L     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4308494..4322009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF28_RS25105 (BF28_4458) gerPD 4308581..4308775 (+) 195 WP_001102340.1 spore germination protein GerPD -
  BF28_RS25110 (BF28_4459) - 4308791..4309177 (+) 387 WP_000902314.1 spore germination protein GerPE -
  BF28_RS25115 (BF28_4460) gerPF 4309220..4309435 (+) 216 WP_001141566.1 spore germination protein GerPF -
  BF28_RS25120 (BF28_4461) - 4309472..4309759 (-) 288 WP_000179950.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  BF28_RS25125 (BF28_4462) addA 4309772..4313497 (-) 3726 WP_000970457.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BF28_RS25130 (BF28_4463) addB 4313494..4317009 (-) 3516 WP_000058552.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BF28_RS25135 (BF28_4464) lepB 4317126..4317689 (-) 564 WP_000751902.1 signal peptidase I -
  BF28_RS25140 (BF28_4465) - 4317746..4318339 (-) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  BF28_RS25145 (BF28_4466) - 4318612..4319193 (-) 582 WP_000428955.1 competence protein ComK -
  BF28_RS25150 (BF28_4467) - 4319475..4319660 (+) 186 WP_000391383.1 hypothetical protein -
  BF28_RS25155 (BF28_4468) - 4319988..4320188 (+) 201 WP_000412674.1 hypothetical protein -
  BF28_RS25160 (BF28_4469) cspA 4320379..4320582 (-) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  BF28_RS25165 (BF28_4470) - 4320934..4321725 (+) 792 WP_000074025.1 phosphotransferase -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134364.58 Da        Isoelectric Point: 5.3316

>NTDB_id=133248 BF28_RS25130 WP_000058552.1 4313494..4317009(-) (addB) [Bacillus cereus E33L]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEKHYEARPNEKFHGEASVTIRTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQILYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=133248 BF28_RS25130 WP_000058552.1 4313494..4317009(-) (addB) [Bacillus cereus E33L]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGCA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTATTGCAAGAAGTTGGT
GGAGCGAGCCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGATATA
ACGTAACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTATATGATTTACAGCTACTATATGATGATTTTGAGCGTGCCTTAATCGGAAAATATTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTCGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGATTTCACTCAT
TTTCACCGCAAGAGCTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
CCGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAACCACGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAAAGCATTACGAAGCGCGTCCGAATGAAAAGTTTCATGGTGAAGCAAGTGTAACGATTCGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTAGCCCGTGAAATTCGTAGACTTGTGGCGGACGAAGATTATCGTTACCGTGATATTGCAGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAGCGTCCGATGTCACACCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATCATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTTCAGAGGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGCGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGGGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTGATTGCCAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTTATTGGCGTGAATGAAGGTG
TCATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTTTGAGCGCTGCAGGTATTGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACATTACTTGCATCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTTATACGTAGCAACGCCT
GAAGTAACGTTGTCATATGTTATGCAGCAACTGCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCGGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTCGCCCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTTGATGC
GCCAGATATCGGGGAGCTTTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTGTAGTAATAGAAGAAATCGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCTAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTTGGTATGGGCGGTACAGGATCACTTCCGCCAATGG
AATTCTCGTTACCTAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGCGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGGTTGGCACTTCA
AATGTTAACTTATTTAGATGTTGTTACTTCAAATGCACATACATGGATGAAAAAAGGCCATGCAGCATCACCAGCTGGTG
TATTATATTTCCACATTCATAACCCAATTGTTGAGGTAAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCTAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAGGGTGTTATCGATATTGCCCCT
TACAAAAAGGGAAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCAGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACACTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.147

100

0.492

Multiple sequence alignment