Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BG04_RS08220 Genome accession   NZ_CP009920
Coordinates   1477687..1480029 (-) Length   780 a.a.
NCBI ID   WP_034648802.1    Uniprot ID   A0A0B6AJG9
Organism   Priestia megaterium NBRC 15308 = ATCC 14581     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1472687..1485029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG04_RS08195 (BG04_1620) - 1473080..1473256 (-) 177 WP_013059305.1 hypothetical protein -
  BG04_RS08200 (BG04_1622) - 1473423..1474307 (+) 885 WP_230586506.1 N-acetylmuramoyl-L-alanine amidase -
  BG04_RS08205 (BG04_1621) dtd 1474298..1474759 (-) 462 WP_016765630.1 D-aminoacyl-tRNA deacylase -
  BG04_RS08210 (BG04_1623) - 1474775..1476973 (-) 2199 WP_013059308.1 RelA/SpoT family protein -
  BG04_RS08215 (BG04_1624) - 1477159..1477671 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  BG04_RS08220 (BG04_1625) recJ 1477687..1480029 (-) 2343 WP_034648802.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BG04_RS08225 (BG04_1626) secDF 1480170..1482407 (-) 2238 WP_016765632.1 protein translocase subunit SecDF -
  BG04_RS08230 (BG04_1627) - 1482545..1482838 (-) 294 WP_016765633.1 post-transcriptional regulator -
  BG04_RS08235 (BG04_1628) spoVB 1482963..1484513 (+) 1551 WP_034654974.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87910.46 Da        Isoelectric Point: 4.6994

>NTDB_id=132952 BG04_RS08220 WP_034648802.1 1477687..1480029(-) (recJ) [Priestia megaterium NBRC 15308 = ATCC 14581]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPILPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKV
RQQIVNDITEEAVEFVENMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRTRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAIDVKRFFEQVFTDQMKVVAFHDSTIDKYKSMLPMEHVVLIQDEEQAKACMFAGEQIVLLDLPPSQ
KWLEALFFQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNHFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=132952 BG04_RS08220 WP_034648802.1 1477687..1480029(-) (recJ) [Priestia megaterium NBRC 15308 = ATCC 14581]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCGGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCTGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACGGATCACCACGAGCCGGGTCCGATTTTACCGGATGCGTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCGGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCTATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCACTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGTA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAATATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGTGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACGTTAAACAGCGA
TGATGTAGAAGAGCTGCGCACACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTGGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAACCGCAGCTGATGGTAGAA
GATATCTCTGTAGATGAGTGGCAGATGTTTGATTATCGCAGTGCTATTGATGTCAAACGTTTCTTTGAGCAGGTATTCAC
GGATCAAATGAAAGTTGTTGCTTTTCACGATTCAACAATTGATAAATACAAGTCGATGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATTGTTTTATTAGATTTGCCTCCTTCACAG
AAGTGGCTTGAAGCTTTGTTTTTCCAAGGTATTCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCACTTTAAATGGTTCTATGGCTTTTTAGCGAAAAAGGGTCCTTTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCGAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCACTTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHHA1 domain (DHHA1)

(305-439)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B6AJG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.385

100

0.554

Multiple sequence alignment