Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS02645   Type   Unclear
Locus tag   RA0C_RS02645 Genome accession   NC_017045
Coordinates   528004..529347 (-) Length   447 a.a.
NCBI ID   WP_004918804.1    Uniprot ID   E4T934
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

Among these genes, RA0C_RS04895, RA0C_RS05130, RA0C_RS09020, RA0C_RS02645, RA0C_RS04870, RA0C_RS05105, RA0C_RS04915, and RA0C_RS04920 were shown to be essential for natural transformation occurrence.


Genomic Context

Location: 523004..534347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS02615 (RA0C_0550) - 524137..524670 (+) 534 WP_004918816.1 helix-turn-helix domain-containing protein -
  RA0C_RS02620 (RA0C_0551) - 524678..525628 (-) 951 WP_004918814.1 alpha/beta fold hydrolase -
  RA0C_RS02625 (RA0C_0552) - 525790..526404 (-) 615 WP_004918812.1 DUF4230 domain-containing protein -
  RA0C_RS02630 (RA0C_0553) - 526411..526941 (-) 531 WP_004918810.1 hypothetical protein -
  RA0C_RS02635 (RA0C_0554) - 526953..527423 (-) 471 WP_004918808.1 nucleoside triphosphate pyrophosphohydrolase family protein -
  RA0C_RS02640 (RA0C_0555) cdd 527460..527945 (-) 486 WP_013446774.1 cytidine deaminase -
  RA0C_RS02645 (RA0C_0556) RA0C_RS02645 528004..529347 (-) 1344 WP_004918804.1 helix-hairpin-helix domain-containing protein Unclear
  RA0C_RS02650 (RA0C_0557) - 529394..529792 (-) 399 WP_004918802.1 GNAT family N-acetyltransferase -
  RA0C_RS02655 (RA0C_0558) - 529833..530171 (-) 339 WP_004918800.1 MerR family transcriptional regulator -
  RA0C_RS02660 (RA0C_0559) - 530230..531207 (-) 978 WP_013446775.1 glycosyltransferase family 2 protein -
  RA0C_RS02665 (RA0C_0560) - 531212..532096 (-) 885 WP_013446776.1 lysophospholipid acyltransferase family protein -
  RA0C_RS02670 (RA0C_0561) - 532195..532746 (+) 552 WP_013446777.1 thioredoxin family protein -
  RA0C_RS02675 (RA0C_0562) - 532789..533136 (-) 348 WP_004918791.1 ArsC/Spx/MgsR family protein -

Sequence

Protein


Download         Length: 447 a.a.        Molecular weight: 52201.04 Da        Isoelectric Point: 9.3223

>NTDB_id=1329 RA0C_RS02645 WP_004918804.1 528004..529347(-) (RA0C_RS02645) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MKKPFSRLEFSMIKKQVLGWSVIAVLLLGIQIFFYFYKKQKEEQPLPELTFTTSVEHEHQDREKLNLTPFNPNQLSLQDW
QNLGFSERQSETILKYKDLLGGSFSSKEELSKCYVISAEKFSELSEFIMLPDKIAKSHENSSHQSFYKSKINVNKPFNPD
LYSVSDWVKLGFSEKQAQSFIKYKNYLGGSFISKEKLRDCYTLSPENYAIIAPYVLLPNKVNPSTTNEIREKKESVVYQP
FDPNDLDLEGWQKLGFSEKQAAVIVNYKQKILKGRFKTLDEVKKCFVISDEKFQALSPYIRIKNEEPSVVETTKPQIKTN
FASVDLNEISYEQLVEFGFTPKAAKSYISFRGLLGGFANKKQILEAYYIDPDLTQKLIETAYLDASKVKKYSLSNAPEEW
LKKHPYFKYHADKIIFYRLTTPNDKKIIKFIKPKPEYLEKMTWYMEE

Nucleotide


Download         Length: 1344 bp        

>NTDB_id=1329 RA0C_RS02645 WP_004918804.1 528004..529347(-) (RA0C_RS02645) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGAAAAAGCCATTCTCTAGACTAGAGTTCAGTATGATAAAAAAGCAAGTGTTAGGGTGGTCTGTTATAGCAGTGTTGCT
TTTAGGGATTCAGATATTTTTTTACTTTTATAAAAAACAAAAAGAAGAACAGCCATTACCTGAACTAACTTTTACCACCT
CGGTAGAACACGAACATCAGGATAGAGAAAAATTAAATCTTACTCCATTTAATCCTAATCAACTAAGTTTGCAAGATTGG
CAAAATTTAGGGTTTTCTGAACGGCAGTCCGAAACAATCCTAAAGTATAAAGATTTGTTGGGAGGTTCTTTTTCGTCTAA
AGAAGAACTATCTAAATGTTATGTAATCTCGGCAGAGAAATTTAGCGAATTGTCAGAATTTATAATGCTTCCTGATAAAA
TAGCTAAATCTCATGAAAATTCATCTCATCAGTCGTTTTATAAGTCTAAAATTAATGTAAATAAGCCTTTTAATCCAGAT
TTATACTCCGTTTCGGATTGGGTGAAACTCGGCTTTTCAGAAAAGCAGGCTCAATCTTTTATCAAGTATAAAAATTATTT
GGGTGGAAGTTTCATTAGTAAAGAAAAATTAAGAGATTGTTATACCTTAAGTCCTGAAAATTATGCCATTATAGCGCCGT
ATGTTCTTTTGCCAAATAAAGTAAATCCTAGTACGACCAACGAAATTAGAGAGAAAAAAGAAAGTGTTGTATATCAACCT
TTTGATCCTAACGACTTGGATTTAGAAGGTTGGCAAAAATTAGGTTTCTCGGAAAAACAAGCTGCTGTTATTGTAAACTA
TAAGCAGAAAATACTCAAAGGAAGATTTAAAACATTAGACGAGGTTAAGAAATGTTTTGTGATTTCTGATGAAAAATTCC
AAGCATTAAGTCCTTATATTAGAATTAAAAATGAGGAACCTTCGGTAGTAGAAACTACAAAACCTCAGATAAAGACTAAT
TTTGCTTCGGTGGACCTTAACGAGATTTCTTATGAGCAATTAGTTGAGTTTGGTTTTACACCTAAGGCAGCTAAAAGTTA
TATTTCGTTTAGAGGGCTACTCGGTGGGTTTGCTAACAAAAAACAAATTCTAGAAGCCTACTATATAGACCCTGATTTAA
CTCAAAAGTTGATAGAAACGGCATATTTAGATGCTTCTAAAGTAAAGAAATATAGTTTATCTAACGCTCCCGAAGAGTGG
CTTAAGAAACATCCTTATTTTAAATATCACGCAGATAAAATTATTTTTTACAGACTTACCACACCCAATGATAAAAAAAT
CATAAAATTTATCAAACCTAAACCAGAGTATTTAGAAAAAATGACTTGGTATATGGAGGAATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(320-377)

Helix-hairpin-helix motif (HHH_3)

(68-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4T934

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]