Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS09020   Type   Unclear
Locus tag   RA0C_RS09020 Genome accession   NC_017045
Coordinates   1900070..1901317 (-) Length   415 a.a.
NCBI ID   WP_014411354.1    Uniprot ID   -
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

Among these genes, RA0C_RS04895, RA0C_RS05130, RA0C_RS09020, RA0C_RS02645, RA0C_RS04870, RA0C_RS05105, RA0C_RS04915, and RA0C_RS04920 were shown to be essential for natural transformation occurrence.


Genomic Context

Location: 1895070..1906317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS10585 (RA0C_1870) - 1895950..1896888 (-) 939 WP_013447133.1 alpha/beta fold hydrolase -
  RA0C_RS09000 (RA0C_1871) - 1897190..1898173 (+) 984 WP_013447134.1 D-alanine--D-alanine ligase -
  RA0C_RS09005 (RA0C_1872) coaD 1898180..1898638 (+) 459 WP_013447135.1 pantetheine-phosphate adenylyltransferase -
  RA0C_RS09010 (RA0C_1873) - 1898667..1899284 (+) 618 WP_013447136.1 trimeric intracellular cation channel family protein -
  RA0C_RS09015 (RA0C_1874) - 1899424..1899963 (+) 540 WP_004919693.1 IS1096 element passenger TnpR family protein -
  RA0C_RS09020 (RA0C_1875) RA0C_RS09020 1900070..1901317 (-) 1248 WP_014411354.1 exo-beta-N-acetylmuramidase NamZ family protein Unclear
  RA0C_RS09025 (RA0C_1876) - 1901305..1902534 (+) 1230 WP_013447139.1 ABC transporter permease -
  RA0C_RS09030 (RA0C_1877) prmA 1902582..1903412 (+) 831 WP_004919686.1 50S ribosomal protein L11 methyltransferase -
  RA0C_RS09035 (RA0C_1878) - 1903446..1904456 (+) 1011 WP_004919683.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  RA0C_RS09040 (RA0C_1879) - 1904648..1905145 (+) 498 WP_004919681.1 GNAT family N-acetyltransferase -
  RA0C_RS09045 (RA0C_1880) - 1905190..1905474 (-) 285 WP_004919680.1 rhodanese-like domain-containing protein -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 47602.91 Da        Isoelectric Point: 9.4562

>NTDB_id=1328 RA0C_RS09020 WP_014411354.1 1900070..1901317(-) (RA0C_RS09020) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MEISKPMYLSLKIKNLLLICLIYLGLSGRNYAQNGNNCPDFKTGADQPELYLPLLKNKKVGVVTNQTGLVLKPQKHHPTT
IDTLSIVDFLRENTIDIRRVFAPEHGFRGEADAGEYVKNGVDTKTGIPIISLYGKNKKPTAEQIQDLDIILFDIQDVGVR
FYTYISTLAYVMEAAAEHNVEVIVLDRPNPHDGYIDGPVLKEKWTSFVGMHPVPIVYGLTIGEYGNMVNGEGWLKNKIKA
KYTLIPMKNYHKKQRYPILRRPSPNLPNDKSINLYPSLCFFEGAEVSVGRGTDYPFQIYGSPWTKKLSYKFTPKPNFGAK
NPPFNGQVCYGENLSEYPKDLRELNLEWIITAYKNYKNPNKPFFIKNLWFDTLAGTDQLRLQIIQGKSETEIKKSWQKDL
QEFEKIRQKYIIYKD

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=1328 RA0C_RS09020 WP_014411354.1 1900070..1901317(-) (RA0C_RS09020) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGGAAATTTCAAAACCTATGTATTTAAGCCTCAAAATTAAAAATTTACTGTTGATTTGCCTAATTTATTTAGGGTTATC
TGGTAGAAACTATGCTCAAAATGGTAACAACTGTCCTGATTTTAAAACAGGAGCCGACCAACCAGAGCTTTATCTTCCTC
TATTAAAAAATAAAAAAGTGGGGGTGGTAACCAACCAAACAGGACTGGTACTGAAACCACAAAAACATCATCCTACTACC
ATAGACACACTTAGCATTGTGGATTTCCTAAGAGAAAACACTATAGACATCAGAAGAGTTTTCGCTCCAGAACACGGTTT
TAGAGGCGAAGCCGATGCAGGGGAATATGTAAAAAATGGTGTAGATACCAAAACAGGTATTCCCATTATTTCTCTTTATG
GTAAAAACAAAAAACCAACTGCAGAACAAATACAAGATTTAGATATTATTCTTTTTGATATTCAAGATGTTGGCGTGAGA
TTTTACACTTATATTTCAACCCTTGCCTATGTGATGGAGGCTGCCGCAGAGCATAATGTAGAAGTGATTGTACTAGATAG
ACCTAACCCTCACGATGGCTACATAGATGGACCCGTATTAAAGGAAAAATGGACAAGTTTTGTGGGTATGCACCCAGTCC
CTATAGTCTATGGATTAACCATTGGCGAGTATGGTAATATGGTAAACGGCGAAGGTTGGCTTAAAAATAAAATAAAAGCC
AAATACACCTTAATCCCAATGAAAAATTACCATAAAAAGCAACGCTACCCTATACTTAGACGCCCTTCTCCTAACTTACC
AAATGACAAGTCTATCAACCTATATCCTTCCCTATGTTTTTTTGAAGGAGCCGAGGTTTCGGTAGGACGCGGCACTGATT
ATCCTTTCCAAATTTATGGTTCACCATGGACTAAAAAATTAAGTTATAAATTCACTCCAAAGCCTAATTTTGGAGCTAAA
AATCCGCCTTTTAACGGGCAAGTATGCTATGGCGAAAACCTCTCCGAATATCCTAAAGATTTGAGAGAACTTAATCTAGA
ATGGATAATTACAGCTTATAAAAACTATAAAAATCCTAATAAACCGTTTTTTATCAAAAATCTTTGGTTTGATACTTTGG
CAGGGACCGACCAACTAAGGCTTCAAATTATACAAGGAAAAAGCGAAACAGAAATCAAAAAATCTTGGCAAAAAGACTTA
CAAGAGTTTGAGAAAATTCGTCAGAAGTATATTATTTATAAAGATTAG

Domains

Predicted by InterproScan.

Protein of unknown function (DUF1343) (NamZ_N)

(60-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]