Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS04070   Type   Unclear
Locus tag   RA0C_RS04070 Genome accession   NC_017045
Coordinates   829428..831257 (+) Length   609 a.a.
NCBI ID   WP_013446859.1    Uniprot ID   E4T9V3
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

RA0C_RS09855, RA0C_RS09870, RA0C_RS09875, RA0C_RS09880, RA0C_RS03780, RA0C_RS07335, RA0C_RS08100, RA0C_RS04070, RA0C_RS07455, RA0C_RS05975, RA0C_RS06070, and RA0C_RS08490 significantly affected the natural transformation frequency of RA.


Genomic Context

Location: 824428..836257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS04050 (RA0C_0850) - 824686..825897 (-) 1212 WP_004918677.1 HD domain-containing protein -
  RA0C_RS04055 (RA0C_0851) - 825951..826958 (-) 1008 WP_004918679.1 o-succinylbenzoate synthase -
  RA0C_RS04060 (RA0C_0852) fbp 826958..827974 (-) 1017 WP_013446857.1 class 1 fructose-bisphosphatase -
  RA0C_RS04065 (RA0C_0853) - 828164..829402 (+) 1239 WP_013446858.1 aspartate kinase -
  RA0C_RS04070 (RA0C_0854) RA0C_RS04070 829428..831257 (+) 1830 WP_013446859.1 lysophospholipid acyltransferase family protein Unclear
  RA0C_RS04080 (RA0C_0856) - 831499..832107 (+) 609 WP_004918689.1 DUF3575 domain-containing protein -
  RA0C_RS04085 (RA0C_0857) - 832112..832318 (+) 207 WP_004918691.1 hypothetical protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70537.56 Da        Isoelectric Point: 9.2029

>NTDB_id=1326 RA0C_RS04070 WP_013446859.1 829428..831257(+) (RA0C_RS04070) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MSLISKKDLIKAAGLDKVGFLKSPIASIIMRLTRINQVNALYDKIKHTEGKDFFKAFVKERNLKYIVFEEDLQKIPKTGP
FILVANHPLGAIDGILMCKILTEIRPDFKIMGNFLLEKIEPMKPFVIPVNPFEGRKEIRSSLQGMKATLKHLEEGGCVGI
FPAGEVSNKNNSFAQIMDKEWEKPALKLMKKAKVPIVPMYFHAKNSKLFYAMSKIHPDLQTLMLPSEMMNKREKPIRVRI
GKPVSVKTIEDCDSVEEMEHLLQKKICLMKTYYDKRQSLADLMKLPNLNLKIPNKSENIVQNIIDETPQEDLIKEIETLK
SEGKMLFDSGNYEVFFSSYQEIPSIMREIGRQRELTFRAVGEGSNLPFDLDEYDKHYHHLFLWDKEAQKLAGAYRMALGK
EVMKKHGIKGFYTSSLFEFDQELQPFFRKVIEMGRAYISMEYQQKPFPLFLLWKGIVHVCLRNPDHKFLMGGVSISNKFS
EFSKSLMIDFMRSHYYDSAVAQYIHPRNEFKIKLKERDKNLFFDGIDSDLGKLDKLIDDLEPEMRLPVLIKKYIKQNAKV
ISFNVDPNFNDAIDGLMYIRISDLPESTIKPVLEEMSEQLGPEEQKKQN

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=1326 RA0C_RS04070 WP_013446859.1 829428..831257(+) (RA0C_RS04070) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGAGTCTCATTTCTAAAAAGGATTTAATAAAAGCTGCTGGTTTAGATAAAGTAGGATTTTTAAAATCTCCTATCGCTTC
TATAATTATGCGTTTGACGAGGATTAATCAAGTTAATGCTCTTTATGACAAAATAAAACATACTGAGGGCAAAGACTTCT
TTAAAGCTTTTGTTAAGGAAAGAAATTTAAAGTATATCGTCTTTGAGGAAGATTTACAAAAAATCCCTAAAACAGGACCT
TTCATATTAGTTGCTAATCACCCTCTAGGAGCGATAGACGGCATACTAATGTGTAAAATCTTAACCGAAATTCGTCCTGA
TTTTAAAATTATGGGCAATTTTCTCTTAGAGAAAATTGAACCTATGAAGCCTTTTGTAATCCCTGTAAATCCTTTTGAAG
GCAGAAAAGAAATCCGTAGTAGTTTGCAAGGAATGAAAGCTACCTTAAAACATCTAGAAGAAGGTGGTTGTGTAGGTATA
TTCCCAGCGGGAGAAGTTTCTAACAAGAATAACTCTTTTGCTCAAATCATGGATAAAGAATGGGAAAAACCAGCTCTTAA
GCTTATGAAAAAGGCTAAAGTCCCCATAGTCCCAATGTATTTTCATGCTAAAAACAGCAAACTTTTTTACGCAATGTCTA
AAATACACCCTGATTTACAAACTCTAATGCTTCCATCTGAAATGATGAACAAAAGAGAAAAGCCTATTAGAGTAAGAATT
GGGAAACCTGTTTCTGTAAAAACCATAGAAGACTGCGACTCTGTGGAGGAAATGGAGCATCTGCTACAAAAGAAAATTTG
CCTTATGAAAACTTATTATGACAAAAGGCAATCGTTGGCAGATTTAATGAAACTCCCAAACCTCAATTTGAAAATTCCAA
ACAAGTCGGAAAACATCGTTCAAAACATCATAGACGAAACACCTCAGGAAGATCTAATTAAGGAAATAGAAACACTGAAA
AGTGAAGGAAAAATGCTCTTTGATAGCGGAAACTATGAAGTCTTTTTCAGTTCTTATCAAGAGATACCTTCCATTATGAG
AGAAATAGGTAGACAAAGAGAACTTACCTTCCGTGCAGTAGGAGAAGGCAGCAACCTTCCTTTTGATTTAGATGAATACG
ATAAACATTATCACCATCTTTTCTTATGGGATAAAGAAGCCCAAAAACTCGCGGGTGCTTACAGAATGGCTCTAGGTAAG
GAGGTGATGAAAAAACACGGCATTAAAGGTTTCTATACCAGCTCCCTTTTCGAGTTTGACCAAGAACTACAACCATTCTT
CCGAAAAGTGATAGAAATGGGAAGAGCTTATATCTCTATGGAGTACCAGCAAAAACCTTTCCCTCTATTTCTTCTTTGGA
AGGGCATCGTGCATGTTTGTTTAAGAAACCCTGATCACAAATTTCTAATGGGTGGTGTAAGTATCAGCAACAAATTTTCG
GAATTTTCTAAATCACTTATGATAGACTTTATGCGTTCTCATTACTATGATTCCGCTGTTGCCCAATACATACACCCAAG
AAATGAATTTAAAATTAAACTAAAAGAAAGAGACAAAAATCTATTTTTTGATGGTATAGACTCAGATTTAGGAAAGCTAG
ACAAACTCATTGATGATTTGGAACCAGAAATGAGATTACCTGTATTGATAAAGAAGTACATTAAACAAAATGCTAAGGTT
ATTTCCTTTAATGTAGACCCAAACTTTAATGACGCTATTGATGGATTGATGTATATTCGTATCAGCGACCTCCCTGAAAG
CACCATTAAGCCTGTATTGGAAGAAATGAGCGAACAACTAGGTCCCGAAGAACAAAAAAAACAAAACTGA

Domains

Predicted by InterproScan.

Acetyltransferase (GNAT) domain (Acetyltransf_5)

(353-452)

Acyltransferase (Acyltransf_2)

(29-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4T9V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]