Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS08100   Type   Unclear
Locus tag   RA0C_RS08100 Genome accession   NC_017045
Coordinates   1705155..1706798 (-) Length   547 a.a.
NCBI ID   WP_004916512.1    Uniprot ID   A0A161PB86
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

RA0C_RS09855, RA0C_RS09870, RA0C_RS09875, RA0C_RS09880, RA0C_RS03780, RA0C_RS07335, RA0C_RS08100, RA0C_RS04070, RA0C_RS07455, RA0C_RS05975, RA0C_RS06070, and RA0C_RS08490 significantly affected the natural transformation frequency of RA.


Genomic Context

Location: 1700155..1711798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS08080 (RA0C_1687) - 1700886..1701413 (+) 528 WP_004916495.1 gamma carbonic anhydrase family protein -
  RA0C_RS08085 (RA0C_1688) - 1701397..1701942 (+) 546 WP_004916502.1 GNAT family N-acetyltransferase -
  RA0C_RS08090 (RA0C_1689) - 1701970..1704099 (+) 2130 WP_004916507.1 helix-turn-helix domain-containing protein -
  RA0C_RS08095 (RA0C_1690) - 1704123..1705121 (-) 999 WP_004916511.1 aminoglycoside phosphotransferase family protein -
  RA0C_RS08100 (RA0C_1691) RA0C_RS08100 1705155..1706798 (-) 1644 WP_004916512.1 apolipoprotein N-acyltransferase Unclear
  RA0C_RS08105 (RA0C_1692) - 1706856..1707266 (+) 411 WP_004916514.1 VanZ family protein -
  RA0C_RS08110 (RA0C_1693) uvrC 1707256..1709046 (-) 1791 WP_015345294.1 excinuclease ABC subunit UvrC -
  RA0C_RS08115 (RA0C_1694) - 1709066..1710055 (-) 990 WP_004916518.1 cation diffusion facilitator family transporter -
  RA0C_RS08120 (RA0C_1695) coaE 1710068..1710658 (-) 591 WP_013447085.1 dephospho-CoA kinase -
  RA0C_RS08125 (RA0C_1696) - 1710674..1711540 (-) 867 WP_013447086.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 547 a.a.        Molecular weight: 62202.14 Da        Isoelectric Point: 8.9773

>NTDB_id=1325 RA0C_RS08100 WP_004916512.1 1705155..1706798(-) (RA0C_RS08100) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MKYIILALISGLLFSVSWPTYGVPFFIFFAFVPLLMVEHDISKFSSIKRRGWAVFGSSYIAFLVWNSITTGWLYEARNPD
GSHAIFAVLFPVLVNSLLMSLAFQCYHWYKNVKSTYWGLAFFIVIWMSFEKFHLEWEFSWPWLHLGNVFADYPKLIQWYD
TLGATGGSFWILIINVLAFYALRIWEAGRVRKDLIKNISIVSAVIVVPMLISLVKYHNFNEKPIGKVRAVMLQPDLNPYT
EKYQKDSLQIVDELLHLAKEHQTSNIDFYLAPETAFPGYGGLSEKGLKQSTLIKKVEDYLSHQPKSVFVGGVSTYNVFYN
ENEAPKTASYYPSQGIWVENYNSAIQIAPSLPYQLYHKAKLVPGVEIFPYISVLKPLLGEAMLNFGGTISSLGMDKERAV
FTNPYNKGKIAPIICYESIYGEFVTDYVKKGANFLGILTNDSWWGVTQGHRQLLAYAKLRAIETRREVARSANSGISAHI
DAKGEIVADTFYGDQTALVADINLYDGTTFYTRTGDLLSRISIFVLGFLVCYLVIEKLMSKTPQRQK

Nucleotide


Download         Length: 1644 bp        

>NTDB_id=1325 RA0C_RS08100 WP_004916512.1 1705155..1706798(-) (RA0C_RS08100) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGAAATATATTATTCTAGCCCTTATATCAGGACTTTTATTTAGTGTTTCTTGGCCCACTTACGGTGTGCCGTTTTTTAT
ATTTTTTGCCTTTGTGCCTCTACTTATGGTAGAGCATGATATTTCTAAATTCAGTAGTATTAAAAGAAGAGGCTGGGCGG
TTTTTGGCAGTTCTTATATCGCCTTTTTAGTATGGAATAGCATTACCACAGGCTGGCTATACGAAGCCAGAAATCCCGAT
GGCTCTCACGCCATTTTCGCTGTATTATTTCCAGTGTTGGTTAATTCACTTTTGATGAGTTTAGCGTTCCAATGTTACCA
CTGGTACAAAAATGTTAAAAGTACCTATTGGGGATTGGCTTTTTTCATTGTGATATGGATGTCTTTTGAGAAATTCCACC
TTGAATGGGAATTTTCTTGGCCTTGGCTTCATCTAGGCAATGTCTTTGCCGACTATCCTAAACTCATACAGTGGTACGAC
ACATTAGGTGCTACTGGTGGCAGTTTTTGGATTTTAATCATCAATGTTCTTGCATTTTATGCTTTAAGAATTTGGGAAGC
AGGAAGAGTACGAAAAGATTTAATAAAAAACATCAGTATCGTTTCTGCTGTTATTGTTGTGCCTATGCTTATCTCTTTAG
TTAAATATCACAACTTTAATGAAAAACCTATTGGGAAAGTTAGAGCTGTGATGCTACAACCAGACCTTAACCCTTATACC
GAGAAATACCAAAAAGACAGTCTACAAATCGTAGATGAACTGCTCCATCTCGCTAAAGAACATCAGACCTCTAATATAGA
TTTCTATCTTGCTCCAGAAACAGCGTTCCCAGGATACGGAGGATTATCAGAAAAAGGATTGAAGCAAAGCACCCTCATTA
AAAAGGTAGAAGACTACCTTAGCCACCAGCCTAAATCGGTTTTTGTAGGTGGCGTTTCTACTTATAATGTATTCTATAAC
GAGAATGAAGCTCCCAAAACAGCAAGCTATTATCCGTCCCAAGGGATTTGGGTGGAGAACTATAACTCTGCTATACAGAT
TGCTCCTTCTCTGCCCTATCAACTTTATCATAAAGCCAAGCTCGTTCCAGGTGTAGAGATATTCCCTTATATTTCTGTAT
TAAAACCTTTATTGGGAGAGGCTATGCTTAATTTTGGAGGCACAATTTCGTCATTAGGAATGGATAAAGAACGAGCGGTA
TTTACCAACCCTTACAATAAAGGTAAAATAGCACCAATTATCTGTTACGAAAGCATTTATGGTGAGTTTGTTACCGATTA
TGTAAAGAAAGGAGCTAACTTTTTAGGAATTCTCACCAACGACTCTTGGTGGGGTGTTACACAAGGTCATCGCCAGTTAC
TCGCTTATGCAAAACTAAGAGCCATAGAAACTAGACGAGAGGTTGCTCGTTCGGCAAACAGTGGTATATCCGCCCACATA
GATGCCAAAGGAGAGATTGTAGCCGATACTTTCTACGGAGACCAAACCGCTTTGGTTGCCGACATTAACTTATACGACGG
CACTACTTTCTACACCAGAACAGGAGATTTATTATCTAGAATTTCCATTTTTGTTTTAGGATTTTTGGTATGTTACCTTG
TGATAGAAAAATTGATGAGTAAAACACCTCAACGCCAAAAATAG

Domains

Predicted by InterproScan.

Apolipoprotein N-acyltransferase N-terminal domain (LNT_N)

(10-177)

Carbon-nitrogen hydrolase (CN_hydrolase)

(245-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A161PB86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]