Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS03780   Type   Unclear
Locus tag   RA0C_RS03780 Genome accession   NC_017045
Coordinates   764336..765346 (+) Length   336 a.a.
NCBI ID   WP_004920367.1    Uniprot ID   E4T9Q0
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

RA0C_RS09855, RA0C_RS09870, RA0C_RS09875, RA0C_RS09880, RA0C_RS03780, RA0C_RS07335, RA0C_RS08100, RA0C_RS04070, RA0C_RS07455, RA0C_RS05975, RA0C_RS06070, and RA0C_RS08490 significantly affected the natural transformation frequency of RA.


Genomic Context

Location: 759336..770346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS03745 (RA0C_0786) - 760098..760985 (-) 888 WP_013446842.1 exopolyphosphatase -
  RA0C_RS03750 (RA0C_0787) ppk1 760992..763058 (-) 2067 WP_013446843.1 polyphosphate kinase 1 -
  RA0C_RS03755 (RA0C_0788) - 763160..763492 (-) 333 WP_004920362.1 CCC motif membrane protein -
  RA0C_RS03760 (RA0C_0789) - 763512..763790 (-) 279 WP_004920365.1 DUF2752 domain-containing protein -
  RA0C_RS03780 (RA0C_0793) RA0C_RS03780 764336..765346 (+) 1011 WP_004920367.1 N-acetylmuramoyl-L-alanine amidase Unclear
  RA0C_RS03785 (RA0C_0794) - 765372..766187 (-) 816 WP_004920369.1 aminotransferase class IV -
  RA0C_RS03790 (RA0C_0795) - 766258..766644 (-) 387 WP_026090384.1 START-like domain-containing protein -
  RA0C_RS03795 (RA0C_0796) - 766820..768751 (+) 1932 WP_004920376.1 LTA synthase family protein -
  RA0C_RS03800 (RA0C_0797) - 768872..769291 (+) 420 WP_004920378.1 DUF2147 domain-containing protein -
  RA0C_RS03805 (RA0C_0798) - 769364..769909 (-) 546 WP_004920384.1 hypothetical protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 38300.27 Da        Isoelectric Point: 8.6945

>NTDB_id=1321 RA0C_RS03780 WP_004920367.1 764336..765346(+) (RA0C_RS03780) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MRNILFIISLGAVVTSCSTQQKIVAPQPQLQPKTKIAPPLSQNKPKVEPSTKGDFYYKTNIGDITKNDNTTSYGSVVTAK
PKGYKVNYENFPSVAQNFRQRFLILHYTALDNETSVRVLTQRGVSAHYLVSDLPDDDIWQLVDENKRAYHAGISFWRNTT
EMNDNSIGIEIVNKGYTTGTAGEKVFYPFPEHQFKKVAALAKDIVERYNIPPTQILAHSDIAPTRKQDPGPFFPWKRLYD
EYNLGMWYDATTKQNFHETALSDLDFNYNNSTFISKVQNTLRSFGYDITPTGTWDKPSQKVIEAFQYHFRPEKCDGVLDA
ETWAILQALIVKYPGK

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=1321 RA0C_RS03780 WP_004920367.1 764336..765346(+) (RA0C_RS03780) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGCGTAATATTTTGTTTATTATAAGTTTAGGTGCTGTGGTGACTTCTTGTAGCACTCAGCAAAAAATAGTTGCACCTCA
ACCACAATTACAACCAAAGACAAAAATAGCCCCCCCCCTTTCCCAAAACAAACCTAAAGTAGAGCCATCTACTAAAGGGG
ATTTCTATTACAAAACTAATATTGGCGATATTACAAAAAACGATAATACCACCAGCTATGGTTCTGTAGTTACTGCTAAA
CCTAAAGGTTATAAGGTGAATTATGAGAATTTTCCATCTGTAGCTCAAAATTTTAGACAGCGTTTTCTTATTCTCCATTA
TACCGCTCTTGATAACGAGACATCAGTACGAGTGCTTACTCAAAGAGGAGTGAGTGCCCACTATCTTGTAAGTGATTTAC
CTGACGATGATATTTGGCAACTTGTTGATGAAAATAAAAGAGCCTACCATGCAGGTATTAGCTTTTGGAGAAATACCACC
GAAATGAATGATAATTCCATAGGGATAGAAATTGTTAATAAAGGTTATACTACTGGGACAGCAGGAGAGAAAGTCTTTTA
TCCATTTCCTGAACATCAGTTTAAGAAGGTGGCTGCTTTGGCAAAAGATATTGTAGAGCGTTATAATATTCCTCCAACTC
AAATTTTAGCCCACTCTGATATTGCTCCAACTAGAAAGCAAGACCCAGGTCCATTTTTTCCATGGAAACGCCTCTATGAT
GAGTATAATCTAGGAATGTGGTATGATGCAACTACCAAGCAAAATTTTCATGAAACTGCTTTAAGCGATTTAGATTTTAA
TTATAACAATTCTACTTTTATTTCAAAAGTGCAAAATACTTTAAGAAGTTTTGGGTATGACATTACTCCTACTGGAACTT
GGGATAAACCTTCTCAAAAGGTTATAGAAGCTTTCCAGTACCACTTTCGTCCAGAGAAATGCGATGGTGTTTTAGATGCA
GAAACTTGGGCTATATTACAAGCTCTTATAGTGAAGTACCCTGGTAAGTAA

Domains

Predicted by InterproScan.

N-acetylmuramoyl-L-alanine amidase (Amidase_2)

(101-231)

Putative peptidoglycan binding domain (PG_binding_1)

(274-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4T9Q0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]