Detailed information    

experimental Experimentally validated

Overview

Name   endA   Type   Machinery gene
Locus tag   DR_RS16810 Genome accession   NC_001263
Coordinates   1622933..1623640 (+) Length   235 a.a.
NCBI ID   WP_010888238.1    Uniprot ID   Q9RTZ7
Organism   Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539     
Function   cleavage of dsDNA into ssDNA   
DNA processing

Function

Periplasmic endonuclease A (EndA, DR1600) converts the double-stranded DNA (dsDNA) to single-stranded DNA (ssDNA) by degrading one DNA strand.


Genomic Context

Location: 1617933..1628640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR_RS08170 (DR_1598) - 1618563..1621361 (+) 2799 WP_027479871.1 M16 family metallopeptidase -
  DR_RS08175 (DR_1599) - 1621524..1622936 (+) 1413 WP_010888237.1 trypsin-like serine peptidase -
  DR_RS16810 (DR_1600) endA 1622933..1623640 (+) 708 WP_010888238.1 endonuclease I family protein Machinery gene
  DR_RS08180 (DR_1601) - 1623731..1624441 (-) 711 WP_010888239.1 VIT1/CCC1 transporter family protein -
  DR_RS08185 (DR_1602) - 1624595..1625761 (+) 1167 WP_010888240.1 heparan-alpha-glucosaminide N-acetyltransferase domain-containing protein -
  DR_RS08190 (DR_1603) - 1625776..1626807 (+) 1032 WP_034350166.1 hypothetical protein -
  DR_RS08195 (DR_1604) - 1626900..1628123 (+) 1224 WP_051618799.1 polysaccharide deacetylase family protein -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26890.89 Da        Isoelectric Point: 6.2224

>NTDB_id=1319 DR_RS16810 WP_010888238.1 1622933..1623640(+) (endA) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MTASTPQARFLALSELLTRTHRAQPRYNPASEVYPWVDVWPDGKLRSIYSASEHDPQEFIAADLAAQDRRERLAAQESLD
VDTLEDAVPYNCEHVVPQSWFAKREPMRGDLHHLFSCEPNCNSFRGNTPYFDFPDYGEALRTDCGKREPGEFEPAHGKGA
VARATLYFLLRYPGVIRTYSERHLHTLLGWHRADPPGEWEHHRNAAIFGRQGNRNPLIDHPEWAEQIAFGEGLGR

Nucleotide


Download         Length: 708 bp        

>NTDB_id=1319 DR_RS16810 WP_010888238.1 1622933..1623640(+) (endA) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGACGGCGAGCACCCCGCAGGCGCGGTTTCTGGCACTTTCGGAACTGCTGACGCGCACGCACCGGGCGCAGCCGCGCTA
CAACCCGGCGAGCGAGGTCTATCCCTGGGTGGACGTGTGGCCCGACGGCAAGCTGCGGTCCATCTACTCCGCGAGCGAGC
ATGACCCGCAGGAATTCATCGCCGCCGACCTCGCCGCGCAGGACCGCCGCGAACGGCTGGCTGCTCAGGAAAGTCTGGAC
GTGGACACCCTGGAAGACGCGGTGCCCTACAACTGCGAGCACGTGGTGCCCCAGAGCTGGTTCGCCAAGCGCGAGCCGAT
GCGCGGCGACCTGCACCACCTCTTTTCCTGCGAGCCGAACTGCAACTCATTCCGGGGCAACACGCCGTACTTCGATTTTC
CCGACTACGGCGAGGCTCTCAGGACCGACTGTGGCAAGCGCGAGCCCGGCGAATTCGAGCCCGCTCACGGCAAGGGCGCC
GTCGCCCGCGCCACGCTGTACTTCCTGCTGCGCTACCCCGGCGTCATCCGCACCTACAGCGAGCGGCACCTGCACACCCT
GCTCGGCTGGCACCGCGCCGACCCGCCCGGCGAGTGGGAACACCACCGCAACGCCGCCATCTTCGGGCGCCAGGGCAACC
GCAATCCGCTGATTGACCACCCAGAGTGGGCTGAGCAGATTGCTTTTGGGGAAGGGCTGGGGCGTTAG

Domains

Predicted by InterproScan.

Endonuclease I (Endonuclease_1)

(42-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RTZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dhirendra Kumar Sharma et al. (2025) Surviving the storm: exploring the role of natural transformation in nutrition and DNA repair of stressed Deinococcus radiodurans. Applied And Environmental Microbiology 91(1):e0137124. [PMID: 39651863]