Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BWEI_RS02545 Genome accession   NZ_CP009746
Coordinates   475533..477854 (+) Length   773 a.a.
NCBI ID   WP_033709438.1    Uniprot ID   -
Organism   Bacillus mycoides strain WSBC10204     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 470533..482854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWEI_RS02530 (bwei_0527) - 471396..471680 (+) 285 WP_002015311.1 post-transcriptional regulator -
  BWEI_RS02535 (bwei_0528) secDF 471963..474230 (+) 2268 WP_002034137.1 protein translocase subunit SecDF -
  BWEI_RS02540 (bwei_0529) - 474538..475431 (+) 894 WP_002034095.1 cation diffusion facilitator family transporter -
  BWEI_RS02545 (bwei_0530) recJ 475533..477854 (+) 2322 WP_033709438.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BWEI_RS02550 (bwei_0531) - 477920..478432 (+) 513 WP_002015305.1 adenine phosphoribosyltransferase -
  BWEI_RS02555 (bwei_0532) relA 478644..480827 (+) 2184 WP_002015304.1 GTP diphosphokinase -
  BWEI_RS02560 (bwei_0533) - 480845..481285 (+) 441 WP_002034098.1 D-tyrosyl-tRNA(Tyr) deacylase -
  BWEI_RS30120 (bwei_0534) - 481554..481730 (+) 177 WP_001242605.1 hypothetical protein -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87271.61 Da        Isoelectric Point: 4.9271

>NTDB_id=131770 BWEI_RS02545 WP_033709438.1 475533..477854(+) (recJ) [Bacillus mycoides strain WSBC10204]
MLQPKTRWKEKEYNEELVSELANKLQLSPLVTSLFLGRGLNTEDKIVDFLNTTDQEFHDPFLLEGMDRTVERVKKAIENG
EQILIFGDYDADGVSSTTVLYLALKELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFALAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPHKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLNMNDVDELRRRLNEQADVILTAEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGTYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITLVYFSEEVLNKFSLEGYKEHIVHASEVAHFDDQYIVLLDLPNGTDELGD
VFKAGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRDHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=131770 BWEI_RS02545 WP_033709438.1 475533..477854(+) (recJ) [Bacillus mycoides strain WSBC10204]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGAGGAACTAGTGAGTGAATTAGCAAATAAATTGCAACT
ATCACCTCTTGTGACTTCATTATTTCTTGGCAGAGGCCTAAATACAGAAGATAAGATTGTAGACTTTTTAAATACAACAG
ATCAAGAATTTCATGATCCATTTTTACTTGAAGGAATGGATCGGACTGTAGAACGTGTGAAAAAAGCTATTGAAAACGGA
GAACAAATATTAATATTTGGCGATTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTCTCAAAGAATT
AGGTGCAGAAGTAGAGTTTTATATTCCAAATCGTTTTACTGAAGGCTATGGGCCAAATGAAGAAGCGTTTCGTTGGGCGC
ACAGTGCAGGGTTCTCACTAATTATTACTGTCGATACTGGTATCGCAGCGGTACATGAAGCGAAGGTAGCGAAGGAACTT
GGAATAGATCTTATTATTACAGATCATCATGAGCCACCACCAGAATTACCAGAGGCACTTGCGATTATTCACCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCATTATTTAGCGGGTGTTGGTGTTGCATTTAAAGTTGCACATGCACTTTTGGGCCGTG
TGCCAGAGCATTTGTTGGAAATTGCAGTAATCGGTACGGTAGCCGATTTAGTATCGCTTCATGGTGAAAATAGATTGTTA
GTACAGCGTGGACTGAAGCATATGCGAATGACTCGGAATATCGGTTTAAAGGCGTTATTTAAAGTTGCTAACGTTTCGCA
AAGCGAAATTACAGAAGAAAGCATCGGGTTCGCACTCGCACCTCGTATTAATGCAGTTGGTCGTTTGGAAGATGCAACAC
CTGCTGTACACCTTTTATTATCAGAAGATCCAGAGGAAGCGAAAGAACTCGCTGAAGAAATTGATGAGCTGAACAAACTT
CGAAAAGATATTGTAAAGCAAATTACTGAAGAAGCTATCGCTGAAGTCGAAAATAATTTCCCGCCAGAAGAAAATAAAGT
ATTAGTCCTTGCAAAAGAAGGTTGGAATCCAGGGGTAATTGGAATTGTCGCTTCTAAGTTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTAAGCATTGATCCTCATAAAGAAACAGCAAAGGGATCGGCTCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACCTTTCAGATTGCCGTGAATTGTTACCGCATTTCGGAGGTCATCCGATGGCAGCGGGAATGACGCTTAATATGAA
TGATGTGGATGAATTAAGACGCAGGTTAAATGAGCAAGCAGATGTAATTTTAACAGCAGAAGATTTTATTCCGATTACAG
CAGTGGATGCATTTTGTAAAGTAGAGGATGTAACGCTTGCCGCGATTGAGGATATGCAAAAGTTAGCGCCATTTGGCGTA
GGAAATCCGAAACCGCGTATTGCGGTGAAAGATGCCGAATTAGAAAGTATCCGTGCAATTGGATCTGATGGTTCGCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGACACGATTGGATTTGGATTTGGTACTTATGCGAAAGAAATCTCTC
CAGTTGCGAAGGTTTCTGTAGTAGGAGAAGTGTCCATTAATGAATGGAATAACTTTAAAAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGACTGGCGTAGTATGCGTAATGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACATTGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGGCTATAAAGAGCATATCGTGC
ATGCATCAGAGGTAGCTCATTTTGATGATCAATACATTGTGCTACTTGATTTGCCGAACGGAACAGATGAATTAGGTGAT
GTATTTAAGGCTGGATTCCCAGCTCGAATTTACACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACGGTCCCAAC
GAGGGAACATTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGGCAATATGGGGAACAACTGT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAGAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTGATCATAAAT
AA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.713

100

0.528

Multiple sequence alignment