Detailed information    

experimental Experimentally validated

Overview

Name   ddrB   Type   Machinery gene
Locus tag   DR_RS00370 Genome accession   NC_001263
Coordinates   66037..66603 (+) Length   188 a.a.
NCBI ID   WP_027480237.1    Uniprot ID   -
Organism   Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539     
Function   DNA binding   
DNA binding and uptake

Function

The transformation frequency of the cells devoid of DprA, a highly conserved protein among competent species, strongly decreased but was not completely abolished whereas it was completely abolished in ΔdprA ΔrecF, ΔdprA ΔrecO, and ΔdprA ΔddrB double mutants. We propose that RecF and RecO, belonging to the recombination mediator complex, and DdrB, a specific deinococcal DNA binding protein, can replace a function played by DprA, or alternatively, act at a different step of recombination with DprA.


Genomic Context

Location: 61037..71603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR_RS00350 (DR_0066) - 63356..64144 (-) 789 WP_027480238.1 APH(3') family aminoglycoside O-phosphotransferase -
  DR_RS00355 (DR_0067) - 64184..65113 (+) 930 WP_010886715.1 hypothetical protein -
  DR_RS00360 (DR_0068) - 65110..65484 (+) 375 WP_010886716.1 hypothetical protein -
  DR_RS00365 (DR_0069) - 65586..65948 (+) 363 WP_010886717.1 hypothetical protein -
  DR_RS00370 (DR_0070) ddrB 66037..66603 (+) 567 WP_027480237.1 single-stranded DNA-binding protein DdrB Machinery gene
  DR_RS00375 (DR_0071) - 66759..68720 (-) 1962 WP_234944657.1 peptidylprolyl isomerase -
  DR_RS00385 (DR_0072) - 69092..69571 (+) 480 WP_027480236.1 hypothetical protein -

Sequence

Protein


Download         Length: 188 a.a.        Molecular weight: 20830.32 Da        Isoelectric Point: 9.2754

>NTDB_id=1317 DR_RS00370 WP_027480237.1 66037..66603(+) (ddrB) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MLQIEFITDLGARVTVNVEHESRLLDVQRHYGRLGWTSGEIPSGGYQFPIENEADFDWSLIGARKWKSPEGEELVIHRGH
AYRRRELEAVDSRKLKLPAAIKYSRGAKVSDPQHVREKADGDIEYVSLAIFRGGKRQERYAVPGGAAGNGQGRPAPQGQP
AQARPQATAARPAARPPVQPGQEEETPF

Nucleotide


Download         Length: 567 bp        

>NTDB_id=1317 DR_RS00370 WP_027480237.1 66037..66603(+) (ddrB) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGTTGCAGATTGAATTTATCACCGACCTGGGTGCGCGGGTCACGGTGAACGTGGAGCATGAAAGCCGGTTGCTGGACGT
ACAGCGCCACTATGGCCGCCTGGGCTGGACCAGCGGCGAGATTCCGTCCGGGGGCTACCAGTTTCCCATCGAGAACGAGG
CCGACTTCGACTGGTCGCTGATCGGCGCCCGCAAGTGGAAGAGCCCCGAGGGCGAAGAACTCGTCATTCACCGGGGCCAC
GCCTACCGCCGCCGCGAACTCGAAGCCGTGGACAGCCGCAAGCTCAAGCTGCCTGCCGCCATCAAGTACAGCCGCGGCGC
CAAGGTCAGCGACCCGCAGCACGTGCGCGAAAAGGCCGATGGCGACATCGAATACGTCAGCCTCGCCATCTTCCGCGGTG
GCAAGCGGCAGGAGCGCTACGCGGTGCCCGGCGGCGCGGCGGGGAACGGTCAGGGTCGCCCGGCGCCCCAGGGACAGCCC
GCCCAGGCCCGTCCCCAGGCCACTGCCGCTCGCCCCGCCGCTCGGCCCCCGGTGCAGCCCGGTCAGGAAGAAGAAACGCC
GTTCTGA

Domains

Predicted by InterproScan.

DdrB-like protein (DdrB)

(3-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Solenne Ithurbide et al. (2020) Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology 11:1253. [PMID: 32625182]
[2] Claire Bouthier de la Tour et al. (2011) The deinococcal DdrB protein is involved in an early step of DNA double strand break repair and in plasmid transformation through its single-strand annealing activity. DNA Repair 10(12):1223-31. [PMID: 21968057]