Detailed information    

experimental Experimentally validated

Overview

Name   recO   Type   Machinery gene
Locus tag   DR_RS04230 Genome accession   NC_001263
Coordinates   832077..832811 (+) Length   244 a.a.
NCBI ID   WP_010887465.1    Uniprot ID   Q9RW50
Organism   Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539     
Function   homologous recombination; promote later steps in plasmid transformation   
Homologous recombination

Function

The transformation frequency of the cells devoid of DprA, a highly conserved protein among competent species, strongly decreased but was not completely abolished whereas it was completely abolished in ΔdprA ΔrecF, ΔdprA ΔrecO, and ΔdprA ΔddrB double mutants. We propose that RecF and RecO, belonging to the recombination mediator complex, and DdrB, a specific deinococcal DNA binding protein, can replace a function played by DprA, or alternatively, act at a different step of recombination with DprA. RecO and RecR are likely to promote later steps in plasmid transformation.


Genomic Context

Location: 827077..837811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR_RS04205 (DR_0814) - 828617..829549 (-) 933 WP_010887460.1 proline dehydrogenase family protein -
  DR_RS04210 (DR_0815) - 829593..830309 (-) 717 WP_051618822.1 GntR family transcriptional regulator -
  DR_RS04215 (DR_0816) - 830374..830838 (-) 465 WP_010887462.1 MgtC/SapB family protein -
  DR_RS04220 (DR_0817) - 830887..831300 (-) 414 WP_010887463.1 hypothetical protein -
  DR_RS04225 - 831496..831888 (-) 393 WP_027479971.1 hypothetical protein -
  DR_RS04230 (DR_0819) recO 832077..832811 (+) 735 WP_010887465.1 DNA repair protein RecO Machinery gene
  DR_RS04235 (DR_0820) - 832980..833378 (-) 399 WP_010887466.1 hypothetical protein -
  DR_RS04240 (DR_0821) - 833394..834290 (-) 897 WP_034350357.1 alpha/beta hydrolase -
  DR_RS04245 (DR_0822) - 834535..835275 (-) 741 WP_027479969.1 SDR family oxidoreductase -
  DR_RS04250 (DR_0823) - 835370..836209 (+) 840 WP_010887469.1 polyphosphate--glucose phosphotransferase -
  DR_RS04255 (DR_0824) - 836311..837516 (+) 1206 WP_051618821.1 amidohydrolase family protein -
  DR_RS04260 (DR_0825) rpmE 837572..837793 (+) 222 WP_010887471.1 50S ribosomal protein L31 -

Sequence

Protein


Download         Length: 244 a.a.        Molecular weight: 26343.46 Da        Isoelectric Point: 9.2272

>NTDB_id=1316 DR_RS04230 WP_010887465.1 832077..832811(+) (recO) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MRSRTANRSGIVIRRRVTPAGDIIVTLLTPQGKLKAIARGGVKGPLSSSLNLFHHVGVQVYQGPHNDLASVKQAVLEGAL
PTLAEPERYAFAHLMAEFADALFQEGEFSEQAFDLFAASLRGVAHQPDPEWVALVMSYKLLGLAGVIPQTARCARCGAPD
PEHPDPLGGQLLCSKCAALPPYPPAVLDFLRHAVRRTVRASFEQPVPSADRPALWRALEKFVTVQVGGVHSWRQLVPSGV
PVLS

Nucleotide


Download         Length: 735 bp        

>NTDB_id=1316 DR_RS04230 WP_010887465.1 832077..832811(+) (recO) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGCGCTCACGCACCGCCAACCGCAGCGGCATCGTCATCCGCCGCCGGGTGACGCCTGCCGGCGACATCATCGTCACGCT
GCTGACCCCCCAGGGCAAGCTCAAGGCCATCGCGCGTGGCGGGGTCAAGGGGCCGCTGTCGAGCAGCCTCAACCTCTTTC
ACCATGTTGGGGTGCAGGTGTACCAGGGGCCGCACAACGACCTTGCCAGCGTGAAACAGGCGGTGCTGGAGGGCGCCCTG
CCCACCCTGGCCGAGCCTGAGCGCTACGCCTTCGCGCACCTGATGGCCGAATTTGCCGACGCGCTGTTTCAGGAAGGCGA
GTTCTCCGAGCAGGCCTTCGACCTCTTCGCCGCCTCGCTGCGCGGCGTGGCGCACCAGCCCGACCCCGAGTGGGTGGCGC
TCGTCATGAGCTACAAGCTGCTCGGACTGGCCGGGGTGATCCCCCAGACCGCTCGCTGCGCCCGTTGCGGCGCGCCCGAC
CCTGAGCACCCCGACCCCCTCGGCGGACAACTGCTGTGCAGCAAGTGCGCGGCCCTCCCGCCCTACCCGCCCGCCGTCCT
CGACTTCCTGCGCCACGCCGTGCGGCGCACCGTCCGCGCCAGCTTTGAGCAGCCGGTGCCGAGTGCCGACCGCCCCGCGC
TGTGGAGAGCGCTGGAAAAGTTCGTCACGGTGCAGGTCGGCGGCGTCCACAGCTGGCGGCAACTCGTCCCAAGCGGCGTG
CCGGTGCTGAGCTAG

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(86-228)

Recombination protein O N terminal (RecO_N)

(6-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1U5K
  PDB 1W3S
  PDB 2V1C
  PDB 4JCV

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Solenne Ithurbide et al. (2020) Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology 11:1253. [PMID: 32625182]
[2] Yuan Wang et al. (2019) Distinct roles of Deinococcus radiodurans RecFOR and RecA in DNA transformation. Biochemical And Biophysical Research Communications 513(3):740-745. [PMID: 30992133]