Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BF36_RS03615 Genome accession   NZ_CP009720
Coordinates   561474..563813 (+) Length   779 a.a.
NCBI ID   WP_042510843.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain HD682     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 556474..568813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF36_RS03600 (BF36_618) - 557506..557790 (+) 285 WP_000886981.1 post-transcriptional regulator -
  BF36_RS03605 (BF36_619) secDF 558061..560325 (+) 2265 WP_001119059.1 protein translocase subunit SecDF -
  BF36_RS03610 (BF36_620) - 560479..561372 (+) 894 WP_000409725.1 cation diffusion facilitator family transporter -
  BF36_RS03615 (BF36_621) recJ 561474..563813 (+) 2340 WP_042510843.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BF36_RS03620 (BF36_622) - 563861..564373 (+) 513 WP_000346214.1 adenine phosphoribosyltransferase -
  BF36_RS03625 (BF36_623) relA 564584..566767 (+) 2184 WP_001262809.1 GTP diphosphokinase -
  BF36_RS03630 (BF36_624) - 566785..567225 (+) 441 WP_001266954.1 D-tyrosyl-tRNA(Tyr) deacylase -
  BF36_RS29615 (BF36_625) - 567411..567587 (+) 177 WP_001242605.1 hypothetical protein -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88172.75 Da        Isoelectric Point: 4.9330

>NTDB_id=131562 BF36_RS03615 WP_042510843.1 561474..563813(+) (recJ) [Bacillus thuringiensis strain HD682]
MLQPKTRWKEKEYNGERVSELASKLQLSPFVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITTVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSKEVLHKFSLEDYKEHMMHASEVTELDEQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTRDHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=131562 BF36_RS03615 WP_042510843.1 561474..563813(+) (recJ) [Bacillus thuringiensis strain HD682]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGCAAATTACAATT
ATCACCGTTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTATTATATCTTGCCCTACAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTCCGTTGGGCGC
ATAGCGCAGGATTTTCACTCATTATTACTGTTGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTTATTATTACGGATCACCATGAGCCGCCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCACTTCACGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATTGGACTAAAGGCGCTATTTAAAGTAGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGTTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAAACGAATTTCCCGCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGATGGAATCCAGGTGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGGGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAGCAAGCGGAGGTAATTTTAACGGAAGAAGATTTTATCCCAATTACAA
CTGTCGATGCTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCAATCGAGGATATGCAAAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCCATCGGATCAGACGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAGGCAACACTTGATACAATCGGGTTTGGATTTGGAGCGTACGCGAAAGAAATTTCTC
CAGTTGCGAAAGTATCTGTAATCGGGGAAGCGTCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAAGCAAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTAAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACCGAGTTAGATGAGCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGATCACTTTAAATGGTACTATTCATTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAGTTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGCTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.842

99.358

0.525

Multiple sequence alignment