Detailed information    

experimental Experimentally validated

Overview

Name   recF   Type   Machinery gene
Locus tag   DR_RS05615 Genome accession   NC_001263
Coordinates   1096951..1098030 (-) Length   359 a.a.
NCBI ID   WP_010887732.1    Uniprot ID   Q9RVE0
Organism   Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539     
Function   homologous recombination   
Homologous recombination

Function

The transformation frequency of the cells devoid of DprA, a highly conserved protein among competent species, strongly decreased but was not completely abolished whereas it was completely abolished in ΔdprA ΔrecF, ΔdprA ΔrecO, and ΔdprA ΔddrB double mutants. We propose that RecF and RecO, belonging to the recombination mediator complex, and DdrB, a specific deinococcal DNA binding protein, can replace a function played by DprA, or alternatively, act at a different step of recombination with DprA.


Genomic Context

Location: 1091951..1103030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR_RS05595 (DR_1085) - 1093053..1093808 (-) 756 WP_010887728.1 class I SAM-dependent methyltransferase -
  DR_RS05600 (DR_1086) alr 1093855..1094910 (+) 1056 WP_027479629.1 alanine racemase -
  DR_RS05605 (DR_1087) fni 1095075..1095959 (-) 885 WP_227086026.1 type 2 isopentenyl-diphosphate Delta-isomerase -
  DR_RS05610 (DR_1088) - 1096088..1096954 (-) 867 WP_010887731.1 DciA family protein -
  DR_RS05615 (DR_1089) recF 1096951..1098030 (-) 1080 WP_010887732.1 DNA replication/repair protein RecF Machinery gene
  DR_RS05620 (DR_1090) - 1098196..1099266 (+) 1071 WP_010887733.1 GGDEF domain-containing protein -
  DR_RS05625 (DR_1091) - 1099503..1099988 (-) 486 WP_010887734.1 hypothetical protein -
  DR_RS05630 (DR_1092) - 1100063..1100557 (+) 495 WP_034349604.1 hypothetical protein -
  DR_RS05635 (DR_1093) trpS 1101143..1102168 (+) 1026 WP_010887736.1 tryptophan--tRNA ligase -
  DR_RS05640 (DR_1094) - 1102329..1102610 (-) 282 WP_034349694.1 YiaA/YiaB family inner membrane protein -
  DR_RS05645 (DR_1095) - 1102713..1102943 (-) 231 WP_010887738.1 hypothetical protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39141.03 Da        Isoelectric Point: 5.0870

>NTDB_id=1315 DR_RS05615 WP_010887732.1 1096951..1098030(-) (recF) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MGDVRLSALSTLNYRNLAPGTLNFPEGVTGIYGENGAGKTNLLEAAYLALTGQTDAPRIEQLIQAGETEAYVRADLQQGG
SLSIQEVGLGRGRRQLKVDGVRARTGDLPRGGAVWIRPEDSELVFGPPSGRRAYLDSLLSRLSARYGEQLSRYERTVSQR
NAALRGGEEWAMHVWDDVLLKLGTEIMLFRRRALTRLDELAREANAQLGSRKTLALTLTESTSPETYAADLRGRRAEELA
RGSTVTGPHRDDLLLTLGDFPASDYASRGEGRTVALALRRAELELLREKFGEDPVLLLDDFTAELDPHRRQYLLDLAASV
PQAIVTGTELAPGAALTLRAQAGRFTPVADEEMQAEGTA

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=1315 DR_RS05615 WP_010887732.1 1096951..1098030(-) (recF) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGGGGGATGTGCGTCTCTCGGCCCTGTCTACCCTGAATTACCGGAATCTTGCGCCTGGGACGCTGAATTTCCCGGAAGG
CGTGACCGGGATTTATGGGGAGAACGGAGCTGGCAAGACCAACCTGCTCGAGGCCGCCTACCTCGCGCTGACCGGACAGA
CCGACGCGCCGCGTATCGAGCAACTGATTCAAGCGGGCGAGACCGAGGCGTATGTGCGGGCCGATTTGCAGCAGGGCGGC
AGCCTCAGCATTCAGGAAGTGGGGCTGGGGCGCGGGCGGCGGCAACTGAAGGTGGACGGCGTGCGCGCCCGGACCGGCGA
CCTGCCGCGCGGCGGAGCGGTGTGGATTCGCCCGGAAGACAGCGAACTGGTGTTCGGGCCGCCCTCGGGACGCCGGGCGT
ATCTGGACTCGCTGCTCTCGCGCCTCAGCGCCCGCTACGGCGAACAACTCTCGCGCTACGAGCGTACGGTGTCGCAGCGC
AACGCGGCCCTGCGCGGCGGCGAGGAATGGGCGATGCACGTCTGGGACGACGTCCTGCTCAAGCTCGGCACCGAAATCAT
GCTGTTTCGTCGCCGGGCGCTCACGCGGCTCGACGAACTGGCGCGCGAGGCGAACGCCCAGCTCGGCAGCCGCAAGACGC
TCGCGCTTACGCTGACCGAGAGCACCTCGCCCGAAACCTACGCCGCCGACCTGCGGGGCCGCCGCGCCGAGGAATTGGCA
CGCGGCTCCACCGTCACCGGCCCGCACCGCGACGACCTGCTGCTCACCCTGGGCGACTTTCCAGCGAGCGACTATGCCAG
CCGGGGCGAAGGCCGCACTGTCGCGCTCGCGCTGCGCCGCGCCGAACTCGAACTGCTGCGCGAGAAATTCGGCGAAGACC
CCGTTTTGCTCCTCGACGACTTCACCGCCGAACTCGACCCCCACCGCCGCCAGTACCTGCTCGACCTCGCCGCCAGCGTG
CCGCAGGCCATCGTGACCGGCACCGAACTCGCTCCCGGCGCGGCGCTGACGTTGCGGGCGCAGGCGGGGCGCTTTACTCC
AGTGGCAGATGAGGAGATGCAAGCGGAGGGCACGGCGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(7-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 2O5V

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Solenne Ithurbide et al. (2020) Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology 11:1253. [PMID: 32625182]
[2] Yuan Wang et al. (2019) Distinct roles of Deinococcus radiodurans RecFOR and RecA in DNA transformation. Biochemical And Biophysical Research Communications 513(3):740-745. [PMID: 30992133]