Detailed information NTG0131034

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: HKU488_RS07010
• Genome accession: NZ_CP012045
• Coordinates: 1390005..1392389 (-)
• Length: 794 a.a.
• NCBI ID: WP_010922541.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain HKU488
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1385005..1397389

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HKU488_RS06990 (HKU488_01281)	pknB	1385023..1386921 (-)	1899	WP_010922538.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
HKU488_RS06995 (HKU488_01282)	-	1386918..1387658 (-)	741	WP_002983660.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
HKU488_RS07000 (HKU488_01283)	rsmB	1387696..1389018 (-)	1323	WP_002983657.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
HKU488_RS07005 (HKU488_01284)	fmt	1389008..1389943 (-)	936	WP_010922540.1	methionyl-tRNA formyltransferase	-
HKU488_RS07010 (HKU488_01285)	priA	1390005..1392389 (-)	2385	WP_010922541.1	primosomal protein N'	Machinery gene
HKU488_RS07015 (HKU488_01286)	rpoZ	1392454..1392771 (-)	318	WP_002983650.1	DNA-directed RNA polymerase subunit omega	-
HKU488_RS07020 (HKU488_01287)	gmk	1392787..1393422 (-)	636	WP_010922542.1	guanylate kinase	-
HKU488_RS07025 (HKU488_01288)	-	1393532..1395139 (-)	1608	WP_002988954.1	ribonuclease Y	-
HKU488_RS07030 (HKU488_01289)	-	1395269..1396165 (-)	897	WP_011285658.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90315.24 Da
• Isoelectric Point: 9.4042

>NTDB_id=131034 HKU488_RS07010 WP_010922541.1 1390005..1392389(-) (priA) [Streptococcus pyogenes strain HKU488]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKSDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVAYFVTNHLIRIDERPI
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFYRYEMSIRHQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSDNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=131034 HKU488_RS07010 WP_010922541.1 1390005..1392389(-) (priA) [Streptococcus pyogenes strain HKU488]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTATTA
TTGGTTTTGGCCAAGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAG
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGTCTGATAGGGACTTTCTTTTTGAAGGAA
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGAAAAAATAGCTCTTAAGGGCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTAGAGATGTGGTGGCTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCCAATT
GATCGCAGTGAGTCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAGGCGTCAGCTGTTAC
TGAAATTGTTGAACAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCGGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTGCCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATCGATGAGGAGCATGAATCAACCTATAAACAAGAAAGCAACCCTAGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGTGTCTATCATTTTATTCAATTAACGCAAAGAGCAAACCCTCTTGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGTAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGTTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCTTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AACCTATCCCAATTACTTGTCCTGAGTGTCATAGCAATAGTATCCGCTATTATGGCACTGGAACTCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATTCCAGAAGCTAAGATCTTGAGAATGGATGTTGACACCACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGACTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACTTCCTTAAATTTGCCGGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGAGAGGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCTATTCAATTAGCTAAGAAACAAGATTTTGAAGCTTTTTACCGTTATGAGATGTCTATTCGTCATCAAATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGGATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-535)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.46	100	0.471