Detailed information    

experimental Experimentally validated

Overview

Name   tfoY   Type   Unclear
Locus tag   VF_RS08130 Genome accession   NC_006840
Coordinates   1762470..1763057 (+) Length   195 a.a.
NCBI ID   WP_005419819.1    Uniprot ID   -
Organism   Aliivibrio fischeri ES114     
Function   require for natural transformation   
Unclear

Function

Chitohexaose-independent transformation by V. fischeri was observed when tfoX was present in multicopy. The second tfoX paralogue, designated tfoY, is also required for efficient transformation in V. fischeri, but is not functionally identical to tfoX.


Genomic Context

Location: 1757470..1768057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS08110 (VF1569) - 1757477..1758922 (-) 1446 WP_011262142.1 hypothetical protein -
  VF_RS08115 (VF1570) torR 1759102..1759800 (-) 699 WP_005419814.1 two-component system response regulator TorR -
  VF_RS08120 (VF1571) torD 1760038..1760685 (+) 648 WP_011262143.1 molecular chaperone TorD -
  VF_RS08125 (VF1572) purR 1760966..1761979 (+) 1014 WP_011262144.1 HTH-type transcriptional repressor PurR -
  VF_RS08130 (VF1573) tfoY 1762470..1763057 (+) 588 WP_005419819.1 DNA transformation protein TfoY Unclear
  VF_RS08135 (VF1574) - 1763136..1763873 (-) 738 WP_005419820.1 hypothetical protein -
  VF_RS08140 (VF1575) - 1763981..1764283 (+) 303 WP_011262145.1 MGMT family protein -
  VF_RS08145 (VF1576) - 1764352..1764855 (-) 504 WP_047863507.1 YbaY family lipoprotein -
  VF_RS08150 (VF1577) tesB 1765088..1765951 (+) 864 WP_011262147.1 acyl-CoA thioesterase II -
  VF_RS08155 (VF1578) - 1766014..1766481 (-) 468 WP_011262148.1 Lrp/AsnC family transcriptional regulator -
  VF_RS08160 (VF1579) - 1766601..1767665 (+) 1065 WP_011262149.1 PLP-dependent cysteine synthase family protein -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 21906.32 Da        Isoelectric Point: 9.5828

>NTDB_id=1302 VF_RS08130 WP_005419819.1 1762470..1763057(+) (tfoY) [Aliivibrio fischeri ES114]
MDKPVLKDSLRLLSSLGKITSRSMFGGFGIFIDDTMFALVVQDKLHLRASDETINLFKEQGFEPYVYKKRGFPVVTKYFA
ISPECWDEPDSILIQAVVALDVAKKDKEKQKSAGPSRIKDLPNLRLATERMLKKAGITTVEELKNTGSVNAYKAIQQTHS
SSVSDELLWSLEGAIKGMHWSVISAETRNELRKQL

Nucleotide


Download         Length: 588 bp        

>NTDB_id=1302 VF_RS08130 WP_005419819.1 1762470..1763057(+) (tfoY) [Aliivibrio fischeri ES114]
ATGGATAAACCAGTATTAAAAGACTCACTTCGTCTACTATCTTCACTAGGAAAGATCACTTCTCGCTCTATGTTTGGGGG
ATTTGGAATCTTCATTGACGACACCATGTTTGCTCTTGTAGTTCAAGATAAGCTTCATTTAAGAGCAAGTGACGAAACGA
TTAACTTATTTAAAGAACAAGGCTTTGAACCATACGTTTATAAAAAACGTGGATTTCCAGTTGTGACAAAATATTTTGCT
ATATCTCCTGAATGTTGGGATGAACCAGATTCAATTTTGATTCAAGCCGTTGTTGCTTTAGATGTAGCGAAAAAAGATAA
AGAAAAACAAAAATCAGCAGGTCCTTCTCGTATTAAAGATTTACCTAATTTACGACTTGCAACAGAAAGAATGCTGAAGA
AAGCCGGAATTACAACAGTTGAAGAACTGAAAAATACTGGCTCTGTAAATGCTTATAAAGCGATACAGCAAACGCATTCA
AGTTCAGTAAGTGATGAATTATTATGGTCTTTAGAAGGGGCTATAAAAGGAATGCACTGGTCTGTAATTTCTGCCGAAAC
ACGTAATGAATTAAGAAAACAACTATAA

Domains

Predicted by InterproScan.

TfoX C-terminal domain (TfoX_C)

(116-194)

TfoX N-terminal domain (TfoX_N)

(12-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Amber Pollack-Berti et al. (2010) Natural transformation of Vibrio fischeri requires tfoX and tfoY. Environmental Microbiology 12(8):2302-11. [PMID: 21966921]