Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   ACJ74_RS05575 Genome accession   NZ_CP011915
Coordinates   1121700..1122464 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain PSUO2     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1116700..1127464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS05545 (ACJ74_05370) - 1117334..1118692 (-) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -
  ACJ74_RS05550 (ACJ74_05375) - 1118769..1119050 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  ACJ74_RS05555 (ACJ74_05380) - 1119047..1119520 (-) 474 WP_001161647.1 PTS sugar transporter subunit IIA -
  ACJ74_RS05560 (ACJ74_05385) - 1119545..1120291 (-) 747 WP_001296489.1 GntR family transcriptional regulator -
  ACJ74_RS05565 (ACJ74_05390) nikR 1120490..1120891 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  ACJ74_RS05570 (ACJ74_05395) nikE 1120897..1121703 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  ACJ74_RS05575 (ACJ74_05400) amiE 1121700..1122464 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  ACJ74_RS05580 (ACJ74_05405) nikC 1122464..1123297 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  ACJ74_RS05585 (ACJ74_05410) nikB 1123294..1124238 (-) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  ACJ74_RS05590 (ACJ74_05415) nikA 1124238..1125812 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  ACJ74_RS05595 (ACJ74_05420) acpT 1125923..1126510 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=129802 ACJ74_RS05575 WP_001136232.1 1121700..1122464(-) (amiE) [Escherichia coli strain PSUO2]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=129802 ACJ74_RS05575 WP_001136232.1 1121700..1122464(-) (amiE) [Escherichia coli strain PSUO2]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398