Detailed information NTG0001298

Experimentally validated

Overview

• Name: chiA2
• Type: Unclear
• Locus tag: VP_RS15510
• Genome accession: NC_004605
• Coordinates: 45762..48308 (-)
• Length: 848 a.a.
• NCBI ID: WP_005482861.1
• Uniprot ID: Q87K43
• Organism: Vibrio parahaemolyticus RIMD 2210633
• Function: require for natural transformation Unclear

Function

VPA0055 (ChiA2), VP0619 (ChiB), and VPA0832 (Cdx) were essential for the robust growth and high transformation frequency on chitin.

Genomic Context

Location: 40762..53308

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP_RS15490 (VPA0051)	nhaD	40956..42386 (-)	1431	WP_005482814.1	sodium:proton antiporter NhaD	-
VP_RS15495 (VPA0052)	-	42797..43822 (-)	1026	WP_005464923.1	acyltransferase family protein	-
VP_RS15500 (VPA0053)	-	44076..44666 (+)	591	WP_005468576.1	TetR/AcrR family transcriptional regulator	-
VP_RS15505 (VPA0054)	-	44787..45644 (+)	858	WP_005468574.1	alpha/beta fold hydrolase	-
VP_RS15510 (VPA0055)	chiA2	45762..48308 (-)	2547	WP_005482861.1	glycosyl hydrolase family 18 protein	Unclear
VP_RS15515 (VPA0056)	-	48678..49181 (-)	504	WP_005464988.1	DUF2850 domain-containing protein	-
VP_RS15520 (VPA0057)	-	49506..49790 (+)	285	WP_005464991.1	pyrimidine/purine nucleoside phosphorylase	-
VP_RS15525 (VPA0058)	-	49961..50242 (+)	282	WP_005464981.1	hypothetical protein	-
VP_RS15530 (VPA0059)	-	50262..51419 (-)	1158	WP_005482807.1	GGDEF domain-containing protein	-

Sequence

Protein

• Length: 848 a.a.
• Molecular weight: 90061.78 Da
• Isoelectric Point: 4.1857

>NTDB_id=1298 VP_RS15510 WP_005482861.1 45762..48308(-) (chiA2) [Vibrio parahaemolyticus RIMD 2210633]
MIRFNLCAAGVALALSGAAVAAPTAPSVDMYGSNNLQFSKIELAMETTSGYYDMVKYHDQAKITVKFNQWSGTPGDTYNI
YFDGVKVATGAITGGQTTATFDYGQGGLYQMEIEACDATGCSKSDPAEITIADTDGSHLKPLTMNVDPNNKTYNTDPSVV
MGTYFVEWGIYGRNYTVDNMPADNLTHILYGFIPICGPNESVKSVGGNSFNALQTACRGVPDYEVVIHDPWAAYQKSFPQ
AGHEYSTPIKGNYAMLMALKQRNPDLKIIPSIGGWTLSDPFYDFVNKANRDTFVASVKKFLKTWKFYDGVDIDWEFPGGG
GAAADKGDPVNDGPAYVALMRELRAMLDELEAETGRTYELTSAIGVGYDKIEDVNYADAVQYMDYIFAMTYDFYGGWNNV
PGHQTALYCGSFMRPGQCDGSGVDENGEAYKGPAYTADNGIQLLLAQGVPANKLVLGTAMYGRGWEGVTPDTLTDPNDPM
TGTATGKLKGSTAQGVWEDGVIDYKGIKSFMLGANNTGINGFEYGYDAQAEAPWVWNRTTGELITFDDHRSVLAKGSYAK
SLGLAGLFSWEIDADNGDILNAMHEGMAGGVVPQPNRKPTAAAGADQSVTGPASVVLDGSQSKDSDGTIASYAWEQVSGT
AVVLAGANTAKASFDAAEVTVEEQLTFKLTVTDNEGATASDLVVVTVKPAGVVDPVNNAPVAQVVAPATANAGDVVVVDA
SASSDADNDTLTFNWTLPQGLNATVNGSKVTFTAAEYPQDTSLSFTVSVSDGKASSSASATVVVAQHTTDPNPGTCDNAW
DASAVYTGGNQVTHAGKTWEAKWWTQGDDPSKSGEWGVWKEVGVANCN

Nucleotide

• Length: 2547 bp

>NTDB_id=1298 VP_RS15510 WP_005482861.1 45762..48308(-) (chiA2) [Vibrio parahaemolyticus RIMD 2210633]
ATGATTCGATTTAACCTATGTGCAGCTGGGGTTGCTTTAGCGCTATCAGGTGCTGCAGTCGCAGCTCCGACCGCACCAAG
TGTCGATATGTACGGTTCTAATAACCTTCAATTTTCTAAAATTGAACTGGCTATGGAAACCACATCTGGCTACTACGACA
TGGTGAAATACCATGACCAAGCTAAAATCACCGTTAAATTCAACCAGTGGAGCGGTACACCTGGCGACACTTACAACATC
TATTTTGATGGCGTAAAAGTCGCAACAGGCGCTATCACTGGCGGCCAAACCACAGCAACATTTGATTACGGCCAAGGCGG
CTTGTACCAAATGGAAATTGAAGCGTGTGATGCTACAGGCTGTTCGAAAAGTGATCCTGCAGAAATCACCATCGCGGATA
CAGATGGCTCTCACTTGAAGCCACTAACGATGAACGTTGATCCAAACAACAAAACATACAACACCGACCCTAGCGTCGTG
ATGGGTACGTACTTTGTTGAATGGGGCATCTATGGTCGTAACTACACCGTAGACAACATGCCAGCCGACAACTTAACTCA
CATCCTTTACGGCTTTATTCCAATCTGTGGTCCAAACGAATCTGTTAAGTCTGTTGGTGGTAACAGCTTTAACGCTCTTC
AAACTGCATGTCGCGGTGTGCCTGATTACGAAGTGGTAATCCATGACCCATGGGCCGCTTACCAGAAGAGCTTCCCGCAA
GCTGGCCACGAGTACAGCACGCCTATCAAGGGTAACTACGCAATGCTAATGGCATTGAAACAACGTAACCCTGATCTAAA
AATCATCCCATCTATCGGTGGCTGGACACTGTCTGACCCATTCTACGATTTCGTTAATAAAGCGAACCGCGACACATTCG
TGGCATCGGTTAAGAAATTCCTGAAAACTTGGAAATTCTACGATGGTGTAGATATTGACTGGGAATTCCCTGGTGGCGGC
GGTGCTGCAGCAGATAAAGGCGACCCAGTAAACGATGGTCCAGCTTACGTTGCATTGATGCGTGAACTGCGCGCAATGCT
AGATGAGCTAGAAGCAGAAACTGGCCGTACTTACGAGCTAACTTCAGCAATTGGTGTCGGCTACGACAAGATTGAAGACG
TAAACTACGCAGATGCCGTTCAATACATGGACTACATCTTCGCGATGACTTACGACTTCTACGGCGGCTGGAACAACGTT
CCTGGTCACCAAACTGCACTTTACTGTGGTTCGTTCATGCGCCCTGGTCAATGTGACGGCAGCGGCGTAGATGAAAACGG
CGAAGCTTACAAAGGCCCTGCGTATACAGCAGATAACGGTATCCAACTTCTTCTTGCTCAAGGCGTTCCTGCGAACAAGC
TAGTCCTTGGTACTGCAATGTACGGCCGTGGCTGGGAAGGCGTAACACCGGATACGCTCACTGATCCAAACGACCCAATG
ACAGGTACTGCGACAGGTAAACTGAAAGGCAGCACTGCACAAGGTGTTTGGGAAGATGGCGTAATCGACTACAAAGGCAT
TAAGTCATTCATGCTAGGTGCAAACAACACTGGCATTAACGGCTTCGAGTACGGCTACGATGCACAAGCTGAAGCACCAT
GGGTATGGAACCGTACAACGGGTGAGTTGATCACATTTGATGACCATCGCTCAGTGCTAGCAAAAGGTAGCTACGCGAAA
TCTCTGGGTCTTGCTGGTCTATTCTCTTGGGAAATCGACGCGGATAACGGCGACATTCTCAACGCAATGCACGAAGGTAT
GGCTGGCGGTGTGGTTCCTCAACCAAACCGTAAACCAACAGCCGCAGCTGGCGCAGATCAATCGGTAACTGGCCCAGCAA
GTGTGGTTCTAGATGGTAGCCAATCGAAAGACTCTGACGGCACTATCGCGAGTTACGCGTGGGAACAAGTATCTGGCACA
GCAGTGGTACTAGCAGGCGCAAACACAGCGAAAGCAAGCTTTGATGCAGCAGAAGTTACTGTTGAAGAGCAATTAACTTT
CAAGCTGACAGTGACTGACAACGAAGGTGCAACGGCATCTGACCTTGTTGTAGTAACTGTGAAGCCAGCAGGTGTGGTTG
ACCCAGTGAACAACGCACCAGTAGCGCAAGTTGTTGCTCCAGCAACAGCAAACGCTGGTGACGTTGTGGTTGTAGATGCA
TCTGCGTCTAGCGATGCAGACAACGATACTCTTACTTTCAACTGGACTCTGCCTCAAGGTCTGAACGCAACGGTAAACGG
CTCTAAAGTGACGTTCACTGCTGCGGAATACCCGCAAGACACAAGCCTAAGTTTCACTGTAAGTGTGAGCGACGGTAAAG
CGTCTTCATCGGCAAGCGCGACAGTGGTTGTTGCACAGCACACAACCGATCCAAACCCAGGTACTTGTGACAACGCATGG
GATGCGAGCGCAGTGTACACAGGTGGCAACCAAGTGACTCACGCAGGTAAAACGTGGGAAGCGAAATGGTGGACTCAGGG
TGACGATCCAAGCAAGTCTGGCGAATGGGGTGTATGGAAAGAAGTTGGCGTAGCTAACTGTAACTAA

Domains

Predicted by InterproScan.

• Chitinase A, N-terminal domain (ChitinaseA_N) (21-156)

• Carbohydrate binding domain (CBM_5_12) (814-839)

• Glycosyl hydrolases family 18 (Glyco_hydro_18) (162-575)

• PKD domain (PKD_4) (697-784)

• PKD domain (PKD_4) (596-687)

3D structure

• AlphaFold DB: Q87K43

References

• [1]: Anusuya Debnath et al. (2022) Chitin degradation and its effect on natural transformation: a systematic genetic study in Vibrio parahaemolyticus. Canadian Journal of Microbiology 68(8):521-530. [PMID: 35623097]