Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LA59_RS16040 Genome accession   NZ_CP009467
Coordinates   3470781..3471797 (-) Length   338 a.a.
NCBI ID   WP_029789968.1    Uniprot ID   -
Organism   Vibrio harveyi strain ATCC 33843 (392 [MAV])     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3465781..3476797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA59_RS16015 (LA59_16010) aroK 3466637..3467155 (-) 519 WP_005451507.1 shikimate kinase AroK -
  LA59_RS16020 (LA59_16015) pilQ 3467345..3469096 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  LA59_RS16025 (LA59_16020) pilP 3469131..3469646 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  LA59_RS16030 (LA59_16025) pilO 3469636..3470229 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  LA59_RS16035 (LA59_16030) pilN 3470222..3470797 (-) 576 WP_069687521.1 PilN domain-containing protein Machinery gene
  LA59_RS16040 (LA59_16035) pilM 3470781..3471797 (-) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  LA59_RS16045 (LA59_16040) - 3471976..3474546 (+) 2571 WP_069687522.1 penicillin-binding protein 1A -
  LA59_RS16050 (LA59_16045) oxyR 3474613..3475521 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  LA59_RS16055 (LA59_16050) - 3475696..3476424 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38023.40 Da        Isoelectric Point: 6.4206

>NTDB_id=129609 LA59_RS16040 WP_029789968.1 3470781..3471797(-) (pilM) [Vibrio harveyi strain ATCC 33843 (392 [MAV])]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDGYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=129609 LA59_RS16040 WP_029789968.1 3470781..3471797(-) (pilM) [Vibrio harveyi strain ATCC 33843 (392 [MAV])]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACG
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAGGTTGCTACCGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494

Multiple sequence alignment