Detailed information NTG0001288

Experimentally validated

Overview

• Name: lqsT
• Type: Regulator
• Locus tag: LPG_RS12645
• Genome accession: NC_002942
• Coordinates: 2825042..2826304 (+)
• Length: 420 a.a.
• NCBI ID: WP_010948208.1
• Uniprot ID: Q5ZSL1
• Organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
• Function: repress natural transformation Competence regulation

Function

Compared with wild-type L. pneumophila, strains lacking lqsT or both lqsS and lqsT show increased salt resistance, greatly enhanced natural competence for DNA acquisition and impaired uptake by phagocytes.

Genomic Context

Location: 2820042..2831304

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPG_RS12630 (lpg2503)	-	2820300..2820620 (-)	321	WP_010948205.1	hypothetical protein	-
LPG_RS12635 (lpg2504)	ceg32	2820928..2823756 (+)	2829	WP_010948206.1	Dot/Icm T4SS effector Ceg32/SidI	-
LPG_RS12640 (lpg2505)	mesI	2823758..2824645 (+)	888	WP_010948207.1	Dot/Icm T4SS metaeffector MesI	-
LPG_RS12645 (lpg2506)	lqsT	2825042..2826304 (+)	1263	WP_010948208.1	sensor histidine kinase	Regulator
LPG_RS12650 (lpg2507)	-	2826352..2827047 (-)	696	WP_010948209.1	iron-containing redox enzyme family protein	-
LPG_RS12655 (lpg2508)	sdjA	2827446..2829869 (-)	2424	WP_010948210.1	Dot/Icm T4SS effector SdjA	-
LPG_RS12660 (lpg2509)	dupB	2829940..2831133 (-)	1194	WP_010948211.1	Dot/Icm T4SS effector deubiquitinase DupB/LaiF	-

Sequence

Protein

• Length: 420 a.a.
• Molecular weight: 48657.07 Da
• Isoelectric Point: 7.9744

>NTDB_id=1288 LPG_RS12645 WP_010948208.1 2825042..2826304(+) (lqsT) [Legionella pneumophila subsp. pneumophila str. Philadelphia 1]
MQRLKNIFTAIYQYLLKETEDASHQITLFGIVMMINYPLFGVFWKLEHFQLTEEFILRITAALLCACLAFNQFWPRQLLK
FLPVFWYIVLLFCLPYFFAYLTLINNGSTLWLMNCVSAIFFLLLVSSVLGALILLISGVGLAFFHFYILSNNQFVYIPGT
ISLFSLIVTFIAAIIIGALFARDREITYAGRLSGMRMLAGSIAHDLRTPLASIYLQAELQELIVERLNNPEVQKDLKENL
SKITRGIEMSNQLIRMQLNNIQRDKLDTSTFSIYSIKKLLKASLEEYPFKENQKSLIHLNDKNDFSIWIDEVGFKNMMWN
LLKNSLEYIEETHKGEISIWLEQGWEKENFNYLHIKDTAKGLNHKEYEKIFDFFYSGRKEGTGLGLAYCKLLMQAAGGDI
SCKGKKNEFAHFTLKFPKID

Nucleotide

• Length: 1263 bp

>NTDB_id=1288 LPG_RS12645 WP_010948208.1 2825042..2826304(+) (lqsT) [Legionella pneumophila subsp. pneumophila str. Philadelphia 1]
ATGCAAAGGTTAAAAAATATATTTACTGCAATTTACCAATATTTATTAAAAGAAACTGAAGATGCAAGCCACCAAATCAC
TTTATTTGGCATCGTCATGATGATTAATTACCCTCTTTTCGGAGTTTTCTGGAAACTGGAGCATTTTCAACTGACAGAAG
AATTTATTTTACGCATCACTGCAGCCCTCCTCTGTGCCTGTTTGGCATTTAATCAATTTTGGCCAAGACAATTATTAAAA
TTTCTACCTGTTTTTTGGTATATAGTTCTTCTCTTTTGTTTGCCTTACTTTTTTGCTTATTTGACTCTCATAAACAATGG
CTCGACTCTTTGGCTAATGAATTGCGTATCCGCTATCTTTTTTCTTTTATTAGTTTCCAGTGTATTGGGGGCTCTTATAT
TACTTATATCAGGTGTTGGACTTGCTTTTTTTCATTTTTATATTTTATCAAATAATCAGTTTGTCTATATCCCAGGAACC
ATATCACTTTTTAGTTTAATTGTTACGTTTATTGCAGCAATAATCATTGGTGCCTTGTTTGCACGAGATAGAGAAATTAC
CTATGCCGGACGTTTATCGGGTATGCGCATGCTGGCTGGAAGTATCGCTCATGATTTAAGAACGCCGCTTGCCAGTATTT
ATTTACAAGCCGAATTACAGGAATTGATTGTCGAAAGATTAAATAATCCAGAAGTTCAAAAGGACCTGAAGGAAAATTTA
AGCAAAATTACTCGCGGCATAGAAATGAGTAATCAATTAATTCGTATGCAATTAAATAATATTCAACGAGACAAATTAGA
TACCAGTACATTTTCGATTTATTCCATCAAAAAATTGCTCAAGGCGTCTTTAGAAGAATACCCTTTCAAAGAAAATCAAA
AATCATTGATTCATTTAAATGATAAAAATGATTTTTCCATATGGATTGATGAAGTGGGTTTTAAAAATATGATGTGGAAT
TTGCTAAAAAATAGTCTGGAATATATTGAGGAAACCCATAAAGGCGAAATCTCTATCTGGCTAGAACAAGGATGGGAGAA
AGAGAATTTTAATTACCTCCATATAAAAGATACAGCTAAAGGTTTAAATCATAAAGAATATGAAAAAATATTTGATTTCT
TCTATTCTGGCAGAAAAGAAGGAACTGGTTTAGGCTTGGCCTATTGTAAACTTCTGATGCAAGCAGCAGGCGGTGATATT
TCCTGTAAAGGGAAAAAAAATGAATTCGCTCATTTCACTTTAAAATTCCCCAAAATTGATTAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (310-417)

• His Kinase A (phospho-acceptor) domain (HisKA) (198-255)

3D structure

• AlphaFold DB: Q5ZSL1

References

• [1]: Aline Kessler et al. (2013) The Legionella pneumophila orphan sensor kinase LqsT regulates competence and pathogen-host interactions as a component of the LAI-1 circuit. Environmental Microbiology 15(2):646-62. [PMID: 23033905]