Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AS86_RS19640 Genome accession   NZ_CP009351
Coordinates   2731480..2734995 (-) Length   1171 a.a.
NCBI ID   WP_000058587.1    Uniprot ID   A0A9W3P216
Organism   Bacillus thuringiensis HD1002     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2726480..2739995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS86_RS19620 (AS86_2794) - 2726676..2727062 (+) 387 WP_000902327.1 spore germination protein GerPE -
  AS86_RS19625 (AS86_2795) gerPF 2727105..2727320 (+) 216 WP_001141566.1 spore germination protein GerPF -
  AS86_RS19630 (AS86_2796) - 2727458..2727745 (-) 288 WP_000255718.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  AS86_RS19635 (AS86_2797) addA 2727758..2731483 (-) 3726 WP_000572302.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AS86_RS19640 (AS86_2798) addB 2731480..2734995 (-) 3516 WP_000058587.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AS86_RS19645 (AS86_2799) lepB 2735111..2735674 (-) 564 WP_000751896.1 signal peptidase I -
  AS86_RS19650 (AS86_2800) - 2735731..2736324 (-) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  AS86_RS19655 (AS86_2801) - 2736754..2737335 (-) 582 WP_000444446.1 competence protein ComK -
  AS86_RS19660 (AS86_2802) - 2737619..2737804 (+) 186 WP_000391375.1 hypothetical protein -
  AS86_RS19665 (AS86_2803) - 2738155..2738358 (+) 204 WP_000356915.1 hypothetical protein -
  AS86_RS19670 (AS86_2804) cspA 2738560..2738763 (-) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  AS86_RS19675 (AS86_2805) - 2739047..2739541 (+) 495 WP_001060337.1 DUF6584 family protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134211.55 Da        Isoelectric Point: 5.2132

>NTDB_id=128797 AS86_RS19640 WP_000058587.1 2731480..2734995(-) (addB) [Bacillus thuringiensis HD1002]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKIIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVANEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASERLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGQAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIANEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=128797 AS86_RS19640 WP_000058587.1 2731480..2734995(-) (addB) [Bacillus thuringiensis HD1002]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAACGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTACAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTGGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGCTATTATACGATGATTTTGAACGTGCTTTAATTGGAAAATATTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATCAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAATAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGCACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAACTTGTTGCGAATGAAAAATATCGTTACCGAGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGCACGTTATTTACAGATTACAATATCCCGCACTTCATCGATG
AAAAGCGTCCGATGTCACACCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGGTGGACTTCAGAGGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGTTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTATTTTTAGAAGAACTGGATGTTCCGAAGAAGCTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACATTTGTAGAGATGCTTGGTGAAGAGAAA
ATGTCTCTTTCTATGTTTACGGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGACGTGAAAGCAACGTTTATTATAGGTGTAAATGAGGGGG
TCATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCGTCTGAGAGGTTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGACGTAAATGATTTATCACGTTCAGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATACAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTTTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAACTAAGTACAGCAGTAAGTAGAGATTTATATGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCATACGCTCATTTTGCACAGCACGGTTTATCACTTAGAGAGCGTGATATTTTCAAGCTTGATGC
ACCGGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCCATAAAAGAGTGCGAGCATCTTTCTGTTTTAGTAATAGAAGAAATTGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCGAGCGGATTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCACTACCAAATGGTGTGAAGATGGAAGTAGTCGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAGAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACAGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACAGACGTGGATGAAAAAAGGCCAAGCAGCATCACCAGCGGGTG
TATTGTATTTTCATATCCATAATCCAATTGTTGAAATGAAAGGTGACGCATCTGAAGCCGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAATGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCGTGCACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.488

100

0.495

Multiple sequence alignment