Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   BF38_RS15230 Genome accession   NZ_CP009335
Coordinates   2342894..2344720 (+) Length   608 a.a.
NCBI ID   WP_000003394.1    Uniprot ID   A0A0B5NYS6
Organism   Bacillus thuringiensis strain HD1011     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2337894..2349720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF38_RS15210 (BF38_2422) - 2338216..2338890 (-) 675 WP_000362609.1 TerC family protein -
  BF38_RS15215 (BF38_2423) mecA 2339217..2339900 (+) 684 WP_000350720.1 adaptor protein MecA Regulator
  BF38_RS15220 (BF38_2424) - 2339973..2341517 (+) 1545 WP_000799201.1 cardiolipin synthase -
  BF38_RS15225 (BF38_2425) - 2341599..2342843 (+) 1245 WP_000612273.1 competence protein CoiA -
  BF38_RS15230 (BF38_2426) pepF 2342894..2344720 (+) 1827 WP_000003394.1 oligoendopeptidase F Regulator
  BF38_RS34145 (BF38_2427) - 2344840..2345013 (-) 174 WP_001984995.1 hypothetical protein -
  BF38_RS15240 (BF38_2428) - 2345243..2346136 (-) 894 WP_000360640.1 ClpXP adapter SpxH family protein -
  BF38_RS15245 (BF38_2429) - 2346136..2346534 (-) 399 WP_000043381.1 hypothetical protein -
  BF38_RS15250 (BF38_2430) - 2346715..2347293 (-) 579 WP_000191105.1 CYTH domain-containing protein -
  BF38_RS15255 (BF38_2431) - 2347440..2347811 (+) 372 WP_001180009.1 hypothetical protein -
  BF38_RS15260 (BF38_2432) - 2347842..2348480 (+) 639 WP_001081482.1 GTP pyrophosphokinase family protein -
  BF38_RS15265 (BF38_2433) - 2348499..2349296 (+) 798 WP_000673188.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70320.59 Da        Isoelectric Point: 4.6458

>NTDB_id=128694 BF38_RS15230 WP_000003394.1 2342894..2344720(+) (pepF) [Bacillus thuringiensis strain HD1011]
MSEQNTAKTLPDRNEIEESSTWRLEDIFQTDTEWEKEFQAIKELLPKLTEFKGKLGDSANNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVYETYGKYKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLIESVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDMLLKSLHVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIVHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=128694 BF38_RS15230 WP_000003394.1 2342894..2344720(+) (pepF) [Bacillus thuringiensis strain HD1011]
ATGTCTGAACAAAATACAGCAAAAACATTACCAGATCGTAACGAGATTGAAGAATCAAGTACGTGGAGATTGGAAGATAT
TTTCCAAACGGATACAGAGTGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTCGGTGACTCTGCGAACAATTTACTTGAAGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGTTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCGTTAAATGACCGCGCGACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCATATATTGTGCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTAA
AAGAAAATAGAGACTTAAGTGTATATGAACATGCATTAGAAGAAATTACGCGCCAACGTCCGCACGTATTATCTGAAGCG
GAAGAGGCTTTATTAGCAGAAGCATCTGAAGTAATGAGTGCGTCAAGTAATACATTTGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAAGTAGAGATTACACATGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTGCGCGAAGATGCATTTAAAGCTGTATATGAAACGTATGGAAAATATAAGAATACATTTGCAAGTACG
TTAAGCGGAGCTGTGAAGCGTAATAATTTTAATGCGCGCGTTCGTAAATACGACTCTGCACGTCAAGCGGCATTAAGTAA
TAATAATATTCCTGAAGCAGTGTACGATCAACTTATTGAATCTGTAAATGATAATTTACACTTGTTACATCGTTACATTG
ATATTCGTAAGCGTGCACTAGGGCTTGATGAGCTTCATATGTATGATTTATATACACCGCTTGTACCAGAAGTGAAAATG
AACGTGAAGTATGAAGAAGCGCAAGACATGTTATTAAAATCTTTACATGTACTTGGTGATGAGTATGTTGATATTTTAAA
AGAAGCATATGAAAATCGTTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGAGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTACTACACAAGAAAAACGCAACCGCACGTATACGGTGACTATTCAATCTTCGTTGCGGAAGTAGCATCAAC
TTGTAATGAAGCGCTTCTAAACGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATACTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAGCATATTGTTCATAAGAAAGTACAA
GAGGGACATGCGGTTACGCCAGACATGTTAACAGAGATTTACTATGATTTAAATAAGAAATATTTCGGTGATGCTTTAGT
AATCGATGAAGAAATTGGTTTAGAGTGGTCTCGTATTCCGCACTTCTACTACAACTATTACGTATACCAATACGCAACAG
GCTTTAGTGCAGCGACGGCTCTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGTTACATTAATGAGTTC
TTAAAAGCAGGAAGCTCAGATTACCCAATTGAAGTGCTGAAAAAAGCTGGGGTAGATATGGCATCTCCAGAACCAGTAAA
AGAAGCATTGCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAGAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5NYS6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.916

97.533

0.487

Multiple sequence alignment