Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   AW22_RS02170 Genome accession   NZ_CP009300
Coordinates   213394..215733 (+) Length   779 a.a.
NCBI ID   WP_042512186.1    Uniprot ID   -
Organism   Bacillus cereus D17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 208394..220733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW22_RS02155 (AW22_221) - 209426..209710 (+) 285 WP_000886975.1 post-transcriptional regulator -
  AW22_RS02160 (AW22_222) secDF 209981..212245 (+) 2265 WP_001119073.1 protein translocase subunit SecDF -
  AW22_RS02165 (AW22_223) - 212399..213292 (+) 894 WP_000409725.1 cation diffusion facilitator family transporter -
  AW22_RS02170 (AW22_224) recJ 213394..215733 (+) 2340 WP_042512186.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  AW22_RS02175 (AW22_225) - 215781..216293 (+) 513 WP_000346214.1 adenine phosphoribosyltransferase -
  AW22_RS02180 (AW22_226) relA 216504..218687 (+) 2184 WP_001262809.1 GTP diphosphokinase -
  AW22_RS02185 (AW22_227) - 218705..219145 (+) 441 WP_001266953.1 D-tyrosyl-tRNA(Tyr) deacylase -
  AW22_RS31410 (AW22_228) - 219331..219507 (+) 177 WP_001242605.1 hypothetical protein -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88135.78 Da        Isoelectric Point: 4.9682

>NTDB_id=128343 AW22_RS02170 WP_042512186.1 213394..215733(+) (recJ) [Bacillus cereus D17]
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRKVEANVAELPKEKITMVYFSKEVLHKFSLEDYKEHMMHASEVTELDEQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTRDHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=128343 AW22_RS02170 WP_042512186.1 213394..215733(+) (recJ) [Bacillus cereus D17]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCGCTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTATTATATCTTGCCCTACAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTCCGTTGGGCGC
ATAGCGCAGGATTCTCACTTATTATTACTGTTGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTTATTATTACGGATCACCATGAGCCGCCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCACTTCACGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATTGGACTAAAGGCGCTATTTAAAGTAGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGCTGGCTGAAGAAATTGATGAACTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAGGTGGAAAATAATTTCCCGCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGAATTGTAGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCGCACTTCGGGGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAGCAAGCAGAGGTAATTTTAACGGAAGAAGATTTTATCCCAATTACAG
CTGTCGATGCTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCAATCGAGGATATGCAAAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAGGCAACACTTGATACAATCGGGTTTGGATTTGGAGCGTACGCGAAAGAAATTTCTC
CAGTTGCAAAGGTATCTGTAATCGGGGAAGCGTCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTACAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGTAGTATGCGTAAAGTAGAAGCAAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTAAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACCGAGTTAGATGAGCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGATCACTTTAAATGGTACTATTCATTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAGTTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGCTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.972

99.358

0.526

Multiple sequence alignment