Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AAF13_RS25505 Genome accession   NZ_CP011331
Coordinates   5083082..5083549 (-) Length   155 a.a.
NCBI ID   WP_000340167.1    Uniprot ID   A7ZSL1
Organism   Escherichia coli O104:H4 str. C227-11 isolate 368 shch     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5078082..5088549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAF13_RS25480 (AAF13_25480) rpsG 5078223..5078693 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  AAF13_RS25485 (AAF13_25485) fusA 5078790..5080904 (+) 2115 WP_000124700.1 elongation factor G -
  AAF13_RS25490 (AAF13_25490) tuf 5080975..5082159 (+) 1185 WP_000031783.1 elongation factor Tu -
  AAF13_RS25495 (AAF13_25495) bfd 5082342..5082536 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  AAF13_RS25500 (AAF13_25500) bfr 5082609..5083085 (+) 477 WP_000675515.1 bacterioferritin -
  AAF13_RS25505 (AAF13_25505) pilD 5083082..5083549 (-) 468 WP_000340167.1 A24 family peptidase Machinery gene
  AAF13_RS25515 (AAF13_25515) rpsJ 5083928..5084239 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  AAF13_RS25520 (AAF13_25520) rplC 5084272..5084901 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  AAF13_RS25525 (AAF13_25525) rplD 5084912..5085517 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  AAF13_RS25530 (AAF13_25530) rplW 5085514..5085816 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  AAF13_RS25535 (AAF13_25535) rplB 5085834..5086655 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  AAF13_RS25540 (AAF13_25540) rpsS 5086672..5086950 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  AAF13_RS25545 (AAF13_25545) rplV 5086965..5087297 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  AAF13_RS25550 (AAF13_25550) rpsC 5087315..5088016 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  AAF13_RS25555 (AAF13_25555) rplP 5088029..5088439 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16984.17 Da        Isoelectric Point: 8.0293

>NTDB_id=127637 AAF13_RS25505 WP_000340167.1 5083082..5083549(-) (pilD) [Escherichia coli O104:H4 str. C227-11 isolate 368 shch]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDCLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=127637 AAF13_RS25505 WP_000340167.1 5083082..5083549(-) (pilD) [Escherichia coli O104:H4 str. C227-11 isolate 368 shch]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACTGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZSL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361

Multiple sequence alignment