Detailed information NTG0126785

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: WV34_RS08540
• Genome accession: NZ_CP011252
• Coordinates: 1659597..1662008 (+)
• Length: 803 a.a.
• NCBI ID: WP_088612794.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain MT45
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1654597..1667008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WV34_RS08515 (WV34_08315)	-	1656239..1657114 (+)	876	WP_013352211.1	YicC/YloC family endoribonuclease	-
WV34_RS08520 (WV34_08320)	remA	1657191..1657460 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
WV34_RS08525 (WV34_08325)	gmk	1657468..1658082 (+)	615	WP_013352212.1	guanylate kinase	-
WV34_RS08530 (WV34_08330)	rpoZ	1658086..1658289 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
WV34_RS08535 (WV34_08335)	coaBC	1658380..1659600 (+)	1221	WP_071346835.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
WV34_RS08540 (WV34_08340)	priA	1659597..1662008 (+)	2412	WP_088612794.1	primosomal protein N'	Machinery gene
WV34_RS08545 (WV34_08345)	def	1662032..1662514 (+)	483	WP_061573884.1	peptide deformylase	-
WV34_RS08550 (WV34_08350)	fmt	1662519..1663472 (+)	954	WP_013352216.1	methionyl-tRNA formyltransferase	-
WV34_RS08555 (WV34_08355)	rsmB	1663459..1664802 (+)	1344	WP_088612795.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
WV34_RS08560 (WV34_08360)	rlmN	1664806..1665897 (+)	1092	WP_014470411.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
WV34_RS08565 (WV34_08365)	-	1665903..1666664 (+)	762	WP_088612796.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90918.05 Da
• Isoelectric Point: 8.9768

>NTDB_id=126785 WV34_RS08540 WP_088612794.1 1659597..1662008(+) (priA) [Bacillus amyloliquefaciens strain MT45]
MNVAEVIVDVSSKNIDRPFDYKIPDHLRGMIEVGMRVIVPFGPRKLQGFVTGLKDSSGLNGGQMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIRQLFRNQKSLLYSDITDERILSALQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQAFKQLAVLTYFIDGGAKISAAELCQKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKKAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVKLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSKELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQVQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=126785 WV34_RS08540 WP_088612794.1 1659597..1662008(+) (priA) [Bacillus amyloliquefaciens strain MT45]
ATGAATGTGGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGGCCCTTTGATTACAAAATTCCCGATCATTT
AAGAGGGATGATCGAAGTCGGTATGCGGGTGATTGTCCCGTTCGGCCCGCGCAAGCTTCAAGGATTCGTGACCGGTTTGA
AAGATTCGTCTGGATTAAACGGCGGGCAAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACACCCGTTTTGACGGAAGAG
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCGGCCGC
GCTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAGGCAGCTTTTCAGAA
ATCAGAAGAGCCTTCTGTACTCAGATATTACCGATGAACGCATTCTTTCGGCCCTGCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACCTATAAAGTCGCCCAGAAAACGAATAAAAAAATGATCCGCATGATTGAGGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAACAGGCTTTTAAACAGCTTGCCGTGCTTACTTATTTTATTGACGGTGGAG
CAAAGATTTCCGCGGCTGAGCTTTGCCAAAAGACCGATTCCAGTTCAGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAGCCTCTCTCTCTTAC
TGAAGAGCAGCAGGCCGCTTTTGAGCCGATTAAGAAGGCCGTAGCCGATCATGTTCATCATGTCTTTCTGCTTCACGGTG
TGACAGGGAGCGGCAAAACGGAAATTTACCTGCAGTCTATTGAAAAGGTGCTGGCTAAAGGAAAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCATTGACGCCGCAAATGGTGAATCGTTTTAAAGGCCGCTTCGGTTCACAGGTTGCAGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAAATTCAGCGTAAGGAAGTAAAGCTTGTCGTCGGAGCCAGGT
CGGCCATCTTTGCCCCATTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCCTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAAGACGTAGCCATACGCAGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTCGCACGGGCCAAAAAAGGCGTTTATGAGCTGCTCCCGCTGAAACACCGCGTAAATCGCCAAC
TCATGCCGGATGTATCTCTCGTTGATATGAGAGAAGAACTGCGAAACGGAAACAGATCGATGTTTTCAAAAGAGCTGATG
GAACAGCTGGAGGAGAAGCTTGCGAAAAAAGAGCAGGCCGTTCTCTTTCTGAATAAACGGGGATATTCTTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCCCAATGTCCGCATTGCGACATTTCAATGACCTATCACCGCTACGGACAAAGGCTGA
AATGCCATTACTGCGGCCATGAGGAGCCTGTTCCGGGAACGTGCCCGGAATGCGGGAGCGAGCATATCCGCTTTTTCGGC
ACGGGAACGCAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACGAC
CTCAAGAAAAGGCGCTCATGAAAAGCTATTGTCAGCTTTCGGTGAAGGAAAAGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCGGCGGAGAAGACATTTCAGCTGTTGACGCAGGTAAGCGGACGGGCCGGCCGGCATGAAAAACCGGGAACGGTAGT
CATACAAACGTACACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCATGACTATGAAACTTTCTTTCAGCAGGAGA
TGGCGCACAGGCGGGCCCAGTCATATCCGCCTTACTATTATGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCTGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGACCGGACACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATTGCCAGGATCAAAGATAGATATCGCTACCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGGTTC
AGTTGAAAAAAATTCTGGAGCATTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATTGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	83.975	100	0.842