Detailed information NTG0125328

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HW11_RS04340
• Genome accession: NZ_CP008873
• Coordinates: 932201..933349 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F9670
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 927201..938349

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW11_RS04320 (HW11_04355)	-	927938..929122 (-)	1185	WP_016852373.1	cystathionine gamma-synthase	-
HW11_RS04325 (HW11_04360)	-	929119..930492 (-)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
HW11_RS04330 (HW11_04365)	-	930735..931139 (-)	405	WP_003084560.1	NINE protein	-
HW11_RS04335 (HW11_04370)	czcD	931294..932193 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HW11_RS04340 (HW11_04375)	pilU	932201..933349 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HW11_RS04345 (HW11_04380)	pilT	933527..934561 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HW11_RS04350 (HW11_04385)	-	934776..935468 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HW11_RS04355 (HW11_04390)	proC	935480..936301 (+)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
HW11_RS04360 (HW11_04395)	-	936312..936905 (+)	594	WP_003084544.1	YggT family protein	-
HW11_RS04365	-	936902..937198 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125328 HW11_RS04340 WP_003100959.1 932201..933349(-) (pilU) [Pseudomonas aeruginosa strain F9670]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125328 HW11_RS04340 WP_003100959.1 932201..933349(-) (pilU) [Pseudomonas aeruginosa strain F9670]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382