Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HW08_RS28885 Genome accession   NZ_CP008858
Coordinates   6013249..6015381 (-) Length   710 a.a.
NCBI ID   WP_003116137.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F63912     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6008249..6020381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW08_RS28870 (HW08_28090) - 6009901..6011556 (-) 1656 WP_016253982.1 AAA family ATPase -
  HW08_RS28875 (HW08_28085) aroB 6011563..6012669 (-) 1107 WP_016253983.1 3-dehydroquinate synthase -
  HW08_RS28880 (HW08_28080) aroK 6012719..6013237 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HW08_RS28885 (HW08_28075) pilQ 6013249..6015381 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  HW08_RS28890 (HW08_28070) pilP 6015435..6015959 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  HW08_RS28895 (HW08_28065) pilO 6015956..6016579 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  HW08_RS28900 (HW08_28060) pilN 6016576..6017172 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  HW08_RS28905 (HW08_28055) pilM 6017172..6018236 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 710 a.a.        Molecular weight: 77361.89 Da        Isoelectric Point: 5.3155

>NTDB_id=124874 HW08_RS28885 WP_003116137.1 6013249..6015381(-) (pilQ) [Pseudomonas aeruginosa strain F63912]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTMEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SSGEEKENSRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHNGNWNS
YGKNGNMGIKDKEGYNCGPFQGQCTFPTRDNSPTPFVDMGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEVVSQPKV
VTSDKETAKILKGSEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFQNALNGVPPINKNEVNA
KILVNDGETIVIGGVFSNTQTKAVDKVPFLGDLPYLGRLFRRDTVSDTKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2133 bp        

>NTDB_id=124874 HW08_RS28885 WP_003116137.1 6013249..6015381(-) (pilQ) [Pseudomonas aeruginosa strain F63912]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCGTTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGAGACCGGGTCGAGCTAAAGTTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
TTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACGCGGTTGATCATCAACCTGACCACGCT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCTGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATTCAAGAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAACCGATGACCATGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCTGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCTGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATTGCCAAGCTGTTCCAGTCGGTTACT
AGTAGTGGCGAAGAGAAAGAAAATTCTCGTGGTTCGATTACCGTAGATGACCGTACCAACAGCATCATTGCCTATCAGCC
GCAGGAGCGGCTGGACGAGCTACGCCGAATCGTTTCCCAGTTGGACATCCCGGTTCGTCAGGTGATGATCGAGGCCCGTA
TTGTCGAGGCCAACGTGGGGTACGACAAGAGTCTTGGTGTGCGTTGGGGTGGTGCCTACCACAATGGCAACTGGAACAGC
TACGGCAAGAACGGCAACATGGGCATCAAGGATAAGGAAGGTTACAACTGCGGGCCGTTCCAGGGGCAGTGTACGTTCCC
GACCAGAGATAATTCTCCGACCCCATTTGTGGATATGGGGGCCAAGGATGCTACTTCCGGTATCGGTATTGGCTTTATCA
CCGATAACATCATTCTTGACTTGCAATTATCGGCCATGGAGAAAACCGGGAACGGCGAAGTGGTTTCGCAACCCAAGGTC
GTAACTTCTGACAAGGAAACCGCGAAGATCCTAAAGGGATCGGAGATCCCATATCAGGAGGCCAGTTCCAGTGGTGCGAC
CAGCACCTCGTTCAAGGAGGCCGCTCTTTCTCTGGAGGTGACGCCGCAGATCACTCCAGACAATCGGATCATCGTTGAGG
TGAAGGTGACCAAGGATGCTCCCGACTTCCAGAATGCTTTGAATGGAGTTCCTCCGATCAACAAGAACGAGGTTAACGCC
AAGATTCTGGTCAACGATGGTGAAACCATCGTTATCGGCGGTGTATTCAGCAACACCCAGACAAAGGCTGTCGACAAGGT
ACCCTTCCTCGGTGACCTGCCTTACTTGGGCCGTTTGTTCCGCCGCGATACGGTCTCCGACACGAAAAACGAACTTCTGG
TTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(545-701)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

94.818

100

0.954
  comQ Acinetobacter baylyi ADP1

42.368

98.732

0.418
  pilQ Acinetobacter baumannii strain A118

40.621

99.859

0.406
  pilQ Acinetobacter baumannii D1279779

40.621

99.859

0.406
  pilQ Legionella pneumophila strain Lp02

38.102

96.479

0.368
  pilQ Legionella pneumophila strain ERS1305867

38.102

96.479

0.368

Multiple sequence alignment