Detailed information    

experimental Experimentally validated

Overview

Name   ctsM   Type   Auxiliary factor
Locus tag   CJJ81176_RS01050 Genome accession   NC_008787
Coordinates   210284..211375 (+) Length   363 a.a.
NCBI ID   WP_002854420.1    Uniprot ID   A0A5Y6R242
Organism   Campylobacter jejuni subsp. jejuni 81-176     
Function   marking transforming DNA by methylation   
Defense system

Function

ctsM methylates an overrepresented 6-bp sequence in the chromosome. DNA derived from a ctsM mutant transforms C. jejuni significantly less well than DNA derived from ctsM+ (parental) cells. The ctsM mutation itself does not affect transformation efficiency when parental DNA is used, suggesting that CtsM is important for marking transforming DNA, but not for transformation itself. The mutant has no growth defect, arguing against ongoing restriction of its own DNA. We further show that E. coli plasmid and PCR-derived DNA can efficiently transform C. jejuni when only a subset of the CtsM sites are methylated in vitro. Methylation influences DNA uptake, with a slight effect also seen on DNA binding.


Genomic Context

Location: 205284..216375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJJ81176_RS01035 (CJJ81176_0237) uppP 206988..207791 (+) 804 WP_002854708.1 undecaprenyl-diphosphate phosphatase -
  CJJ81176_RS01040 (CJJ81176_0238) thrS 207922..209730 (+) 1809 WP_002869067.1 threonine--tRNA ligase -
  CJJ81176_RS01045 (CJJ81176_0239) infC 209727..210245 (+) 519 WP_002851726.1 translation initiation factor IF-3 -
  CJJ81176_RS01050 (CJJ81176_0240) ctsM 210284..211375 (+) 1092 WP_002854420.1 DNA adenine methylase Auxiliary factor
  CJJ81176_RS09145 vvfC 211415..214867 (+) 3453 Protein_200 autotransporter serine protease VvfC -
  CJJ81176_RS01085 (CJJ81176_0249) argC 214948..215976 (+) 1029 WP_002869256.1 N-acetyl-gamma-glutamyl-phosphate reductase -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 42306.78 Da        Isoelectric Point: 9.6734

>NTDB_id=1222 CJJ81176_RS01050 WP_002854420.1 210284..211375(+) (ctsM) [Campylobacter jejuni subsp. jejuni 81-176]
MKENPSFLKEQIITYLGNKRALLSFLNNGFKVAKKELGKDKFSFCDIFSGSGVVSRFAKAHSNYILANDLEDYSKLINEC
YLANKDKDLLQNIKKYYKNLIQNLDFQKGFISELYAPKDDDDIKKNERVFYTFKNALYLDTIRQKIENEIPKELRHFFIA
PLIYEASVHSNTSGVFKGFYKGKDGIGKFGGEGQNALKRIKGEIELKIPIFSNFSCEFEVMQKDANMLAKELDFFDVVYL
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKSKFAQDALFELINDLKARVILLSYNCEGFVKKEIF
LKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLHEQLYILVKN

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=1222 CJJ81176_RS01050 WP_002854420.1 210284..211375(+) (ctsM) [Campylobacter jejuni subsp. jejuni 81-176]
TTGAAAGAAAATCCTTCTTTTTTAAAAGAGCAAATTATAACTTATCTTGGAAATAAAAGAGCACTTTTAAGTTTTTTAAA
TAATGGCTTTAAGGTAGCTAAAAAAGAACTTGGTAAAGATAAATTTAGTTTTTGTGATATATTTAGTGGCTCAGGTGTTG
TAAGTCGTTTTGCAAAAGCACACTCAAATTATATTTTAGCCAATGATTTAGAAGATTATTCAAAACTCATCAATGAGTGT
TATCTTGCTAATAAAGACAAAGATTTACTTCAAAATATAAAAAAGTATTATAAAAATTTAATCCAAAATTTAGATTTTCA
AAAAGGTTTTATCAGTGAGCTTTATGCTCCAAAAGATGATGATGATATTAAAAAAAATGAAAGGGTTTTTTATACTTTTA
AAAATGCTTTATATCTTGATACTATAAGGCAAAAAATCGAAAATGAAATTCCAAAAGAATTGAGGCATTTCTTTATAGCT
CCTTTGATTTATGAAGCCAGTGTTCATTCAAATACTAGCGGAGTTTTTAAAGGTTTTTATAAGGGTAAGGATGGTATCGG
AAAATTTGGAGGAGAAGGACAAAATGCACTTAAACGCATTAAAGGAGAAATAGAGCTTAAAATCCCTATTTTTTCAAATT
TTTCTTGTGAATTTGAAGTGATGCAAAAGGATGCAAACATGCTTGCAAAAGAACTTGATTTTTTTGATGTAGTGTATCTT
GACCCCCCTTATAATCAGCACCCTTATAGTTCTAATTATTTTATGCTAAATTTAATTGCAAATTATAAAAAACCTGAAGA
AATTTCTAAAATCAGTGGCATTCCAAAGGATTGGAACCGCAGTACTTTTAATAAGTCAAAATTTGCCCAAGATGCTTTAT
TTGAACTTATTAATGATTTAAAAGCAAGAGTGATTTTACTTTCTTATAATTGTGAAGGTTTTGTAAAAAAAGAAATTTTT
TTAAAGCGTTTAAGTACTCTTGGAAAATGTCGGATTTTAGAACAAAAATATAATACTTTTAGAGCTTCTCGCAATCTTAA
AAATCGTAATATCCATCTTCATGAACAACTTTATATTTTAGTTAAAAATTAA

Domains

Predicted by InterproScan.

D12 class N6 adenine-specific DNA methyltransferase (MethyltransfD12)

(15-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Y6R242

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Jessica M Beauchamp et al. (2017) Methylation-dependent DNA discrimination in natural transformation of Campylobacter jejuni. Proceedings of The National Academy of Sciences of The United States of America 114(38):E8053-E8061. [PMID: 28855338]