Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   EN18_RS21975 Genome accession   NZ_CP007634
Coordinates   2364340..2365200 (+) Length   286 a.a.
NCBI ID   WP_001884047.1    Uniprot ID   Q9KNL3
Organism   Vibrio cholerae strain 2012EL-2176     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2359340..2370200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EN18_RS10705 (EN18_16575) greB 2359868..2360353 (-) 486 WP_000850496.1 transcription elongation factor GreB -
  EN18_RS10710 (EN18_16580) - 2360598..2362919 (+) 2322 WP_000030087.1 Tex family protein -
  EN18_RS10715 (EN18_16585) - 2363011..2363481 (-) 471 WP_000961392.1 hypothetical protein -
  EN18_RS10720 (EN18_16590) bioH 2363643..2364404 (-) 762 WP_001240449.1 pimeloyl-ACP methyl ester esterase BioH -
  EN18_RS21975 comF 2364340..2365200 (+) 861 WP_001884047.1 ComF family protein Machinery gene
  EN18_RS10730 (EN18_16600) nfuA 2365288..2365875 (+) 588 WP_000070178.1 Fe-S biogenesis protein NfuA -
  EN18_RS10735 (EN18_16605) nudE 2366014..2366562 (+) 549 WP_000095812.1 ADP compounds hydrolase NudE -
  EN18_RS10740 (EN18_16610) cysQ 2366585..2367412 (+) 828 WP_000106799.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  EN18_RS10745 (EN18_16615) - 2367464..2368222 (-) 759 WP_000817146.1 type II secretion system protein N -
  EN18_RS10750 (EN18_16620) - 2368224..2368721 (-) 498 WP_000661339.1 type II secretion system protein M -
  EN18_RS10755 (EN18_16625) gspL 2368731..2369942 (-) 1212 WP_001288747.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32545.47 Da        Isoelectric Point: 8.0588

>NTDB_id=121973 EN18_RS21975 WP_001884047.1 2364340..2365200(+) (comF) [Vibrio cholerae strain 2012EL-2176]
MPQPCTNTRSCPCPLTCQYNAMVTVSPSISIFIHSANWQDEAHYPDSWLAMLTHWLRKTTAPLLTPECHLCRLALDTNSP
FGVCSACQAWLEHGYRCARCGLPTLTPVDQCGQCLGQPPPWRKLMCVGDYRFPLSDAVHQLKYQRQFWQAPRLAKLLATQ
INEPAPLLCSVPLHWRRRWQRGFNQSDLLARELANMLNVEYDHQLFARRRATPHQQGLSKAQRIHNLRDAFVLNHPPNQP
HVAIVDDVVTTGSTIRHLCDLLLDVGVQSIDIYCICRTPEPKDSHG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=121973 EN18_RS21975 WP_001884047.1 2364340..2365200(+) (comF) [Vibrio cholerae strain 2012EL-2176]
ATGCCCCAGCCGTGTACCAATACCAGATCTTGCCCTTGTCCACTGACTTGCCAATACAACGCCATGGTCACAGTTTCTCC
GTCTATTTCCATCTTCATCCACTCTGCCAATTGGCAAGATGAAGCACATTACCCCGATTCTTGGCTCGCTATGTTAACCC
ATTGGCTCAGAAAAACTACCGCACCACTGCTGACTCCGGAATGTCATCTCTGTCGACTGGCGCTAGATACAAATTCGCCG
TTTGGCGTCTGCTCTGCCTGCCAAGCTTGGCTTGAGCACGGTTATCGCTGCGCACGCTGCGGTTTACCGACTCTTACCCC
AGTTGATCAGTGCGGGCAGTGTTTGGGTCAGCCTCCGCCGTGGCGAAAACTGATGTGTGTCGGCGATTACCGCTTTCCAC
TTAGTGATGCGGTGCATCAACTCAAATACCAACGCCAGTTTTGGCAAGCGCCGCGCTTGGCTAAGCTACTCGCCACCCAG
ATTAACGAGCCAGCCCCGTTGCTGTGCAGCGTGCCACTGCATTGGCGGCGACGCTGGCAACGCGGCTTTAATCAAAGCGA
TCTGCTGGCGCGCGAGTTAGCCAATATGCTGAACGTTGAATATGACCACCAACTCTTTGCACGCCGCCGAGCGACACCGC
ATCAACAAGGACTCAGCAAGGCGCAGCGAATCCACAACCTACGCGATGCGTTTGTGCTGAATCATCCGCCAAACCAACCA
CATGTTGCGATTGTGGACGATGTGGTGACCACAGGCAGTACGATCCGACATTTATGCGATTTACTGCTTGATGTCGGTGT
GCAAAGCATTGATATTTACTGCATATGCCGCACTCCCGAGCCGAAAGATAGCCACGGTTGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(189-281)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KNL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Vibrio cholerae strain A1552

100

100

1
  comF Vibrio campbellii strain DS40M4

47.059

83.217

0.392

Multiple sequence alignment