Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   EG66_RS00100 Genome accession   NZ_CP007605
Coordinates   14242..16605 (+) Length   787 a.a.
NCBI ID   WP_023591478.1    Uniprot ID   V5NMV1
Organism   Helicobacter pylori strain BM012B     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9242..21605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG66_RS00070 (EG66_00080) groES 9261..9617 (-) 357 WP_000671934.1 co-chaperone GroES -
  EG66_RS00075 (EG66_00085) dnaG 9904..11583 (+) 1680 WP_023591475.1 DNA primase -
  EG66_RS00080 (EG66_00090) - 11580..12632 (+) 1053 WP_023591476.1 MnmA/TRMU family protein -
  EG66_RS00085 (EG66_00100) - 12723..13550 (+) 828 WP_023591477.1 DUF5718 family protein -
  EG66_RS00090 (EG66_00105) comB2 13696..13977 (+) 282 WP_001272690.1 TrbC/VirB2 family protein Machinery gene
  EG66_RS00095 (EG66_00110) comB3 13977..14240 (+) 264 WP_000584956.1 hypothetical protein Machinery gene
  EG66_RS00100 (EG66_00115) comB4 14242..16605 (+) 2364 WP_023591478.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  EG66_RS00105 (EG66_00120) - 16927..18267 (+) 1341 WP_023591479.1 COG3014 family protein -
  EG66_RS00110 (EG66_00125) cheV1 18375..19340 (+) 966 WP_023591480.1 chemotaxis protein CheV1 -
  EG66_RS00115 (EG66_00130) nspC 19337..20554 (+) 1218 WP_038419548.1 carboxynorspermidine decarboxylase -
  EG66_RS00120 (EG66_00135) lpxE 20577..21110 (-) 534 WP_226975759.1 lipid A 1-phosphatase LpxE -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90175.64 Da        Isoelectric Point: 8.7284

>NTDB_id=121583 EG66_RS00100 WP_023591478.1 14242..16605(+) (comB4) [Helicobacter pylori strain BM012B]
MLEKLLSAIKQKVSNYFLGVLPKSYSMSEENNILGLYDEHFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVARLVVKRRKIDHQQNIQTDSKYLQTILNQFENKEVYENQYFLVLESAHSLQGVLEHKKKSLMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLSSKEVLNFYAEYINGFDLPLKPLVGGYLSDSYIASSTTFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAIGALLYQETPLDIIFSIEPMSVNKTLGFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNILIKAPSLEDLDAQTSLVLGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGQSFSINPFCLEPNLQNLEFLQSFFLSMFDLAPSRDKEALEDMNAISSAIKSLYETLYPKDFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDAFNSNAFLNVINLDAITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNTKINALITQARKANGVVVLALQDIHQLSGVKNAHSFLSNMGTLILYPQKNARELKHHFNVPLSETEIS
FLENTPLYARQVLVKNLGNGNSNMIDVSLESLGRYLKIFNSDSSHVNKVKALQKGYPTEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=121583 EG66_RS00100 WP_023591478.1 14242..16605(+) (comB4) [Helicobacter pylori strain BM012B]
ATGTTAGAAAAGCTTTTGAGCGCTATCAAACAAAAAGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACATTTTAGGCTTGTATGATGAGCATTTCTTGCTCACTAAAAACGAAAACTTAGTGGGCATCCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACAGAGCAATTGCAAGATCTTTTCACCGAGCGCCAGATGGCGTTGGAT
TCTTTAGAAAAAGTCGTGGCGCGCCTGGTGGTTAAAAGGCGTAAAATTGATCACCAACAAAACATTCAAACTGATTCTAA
ATACTTGCAAACGATTTTGAATCAATTTGAAAACAAAGAAGTGTATGAAAATCAGTATTTTTTAGTTTTAGAAAGCGCAC
ACTCTTTACAGGGCGTTTTAGAGCATAAGAAAAAATCCTTAATGCATGCCAATAGGGAAAATTTTAAGGATATTCTCTCT
TATAAAGCGCATTTTTTACAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAATTATGCCCCCAAACTCTTAAGCTC
CAAAGAGGTTTTGAATTTTTATGCGGAATACATTAACGGGTTTGATCTCCCCTTAAAACCCTTAGTAGGGGGGTATTTGA
GCGATAGCTATATCGCTAGTTCTACCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAATCAAAAAACCTATAAC
CGCTTGATTGGCATTAAAGCTTATGAGAGCGAAAGGATCACTTCTATAGCGATCGGAGCACTTTTATACCAAGAGACGCC
ACTAGATATTATCTTCTCTATAGAGCCTATGAGCGTCAATAAAACGCTGGGTTTTTTAAAAGAGAGGGCCAAATTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTGCTAGAATACCAAGAATTAGTCAAAACCAAACGCCTGTCCATGCAAAAATTCGCC
CTAAACATTCTTATCAAAGCCCCTAGTTTAGAGGATTTAGACGCTCAAACCAGCTTGGTTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAGGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACCTCTAAAGCCCTAGCGTGTTTAATGGTGTTTGAAAGGCAAAATTTAGGTTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAATAACGCTAAAGAAATCGCCAGAGTGAACGGGCATACTCTAATCATAGGGGCTACCGGGAGCGGTAAAAGCA
CGCTGATTAGTTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCCTTTGATAGGATGCAGGGGCTG
TATTCTTTCACAAAATTTTTTAAAGGGCATTACCATGACGGCCAATCTTTTAGCATCAACCCCTTTTGTTTAGAGCCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTCTTTTTGAGCATGTTTGATCTTGCCCCTTCAAGGGATAAAGAAGCCTTGG
AAGACATGAATGCGATTTCTAGCGCGATTAAGAGCCTTTATGAGACCTTATACCCTAAAGATTTTAGTTTGCTAGACTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAATTGGGTTTGAGTTTAGAGCCGTATTTGAATAACCCCCTTTTTAACGC
TTTGAATGACGCATTCAACTCTAACGCTTTTTTAAATGTGATAAACCTAGATGCCATCACCCAAAACCCTAAAGACTTGG
GGCTTTTAGCCTATTATTTGTTTTATAAAATCTTAGAAGAATCCAGGAAAAACGACAGCGGTTTTTTGGTTTTTTTAGAC
GAGTTTAAATCCTATGTGGAAAACGACTTATTAAACACTAAAATTAACGCTTTAATCACGCAAGCCAGAAAAGCTAATGG
CGTAGTGGTGTTGGCCTTGCAAGATATTCACCAATTAAGCGGGGTTAAAAACGCTCATAGTTTTTTAAGCAACATGGGGA
CTCTTATTTTGTATCCGCAAAAAAACGCTAGGGAGTTGAAACACCATTTCAATGTGCCTTTGAGCGAAACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAGGTTTTAGTCAAAAATTTGGGTAATGGGAATTCTAACATGATTGATGT
GAGTTTGGAAAGTTTGGGGCGTTATTTGAAAATCTTTAATTCAGACTCTAGCCATGTGAATAAAGTGAAAGCGTTACAAA
AAGGCTACCCTACAGAGTGGCGTGAGAAACTTTTGAAGAGCTAG

Domains

Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V5NMV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.713

100

0.977

Multiple sequence alignment