Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   CY96_RS04830 Genome accession   NZ_CP007512
Coordinates   956642..960157 (+) Length   1171 a.a.
NCBI ID   WP_044584220.1    Uniprot ID   A0A9W3KZV0
Organism   Bacillus bombysepticus str. Wang     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 951642..965157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CY96_RS04795 (CY96_04865) - 952193..952687 (-) 495 WP_000182390.1 DUF6584 family protein -
  CY96_RS04800 (CY96_04870) cspA 952971..953174 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  CY96_RS04805 (CY96_04875) - 953372..953575 (-) 204 WP_000356922.1 hypothetical protein -
  CY96_RS04810 (CY96_04880) - 953924..954109 (-) 186 WP_000391372.1 hypothetical protein -
  CY96_RS04815 (CY96_04890) - 954394..954975 (+) 582 WP_000444439.1 competence protein ComK -
  CY96_RS04820 (CY96_04895) - 955312..955905 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  CY96_RS04825 (CY96_04900) lepB 955962..956525 (+) 564 WP_000751910.1 signal peptidase I -
  CY96_RS04830 (CY96_04905) addB 956642..960157 (+) 3516 WP_044584220.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CY96_RS04835 (CY96_04910) addA 960154..963879 (+) 3726 WP_044584221.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CY96_RS04840 (CY96_04915) - 963892..964179 (+) 288 WP_000255727.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  CY96_RS04845 (CY96_04920) gerPF 964317..964532 (-) 216 WP_001141566.1 spore germination protein GerPF -
  CY96_RS04850 (CY96_04925) - 964575..964961 (-) 387 WP_000902319.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134108.32 Da        Isoelectric Point: 5.1777

>NTDB_id=120195 CY96_RS04830 WP_044584220.1 956642..960157(+) (addB) [Bacillus bombysepticus str. Wang]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVAEENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKGLDLTEVYYGLALQMLTYLDVVTSNAQTWLKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=120195 CY96_RS04830 WP_044584220.1 956642..960157(+) (addB) [Bacillus bombysepticus str. Wang]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAACGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTACAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATACGATGATTTTGAACGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CCTACAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTTGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATCAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCACATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCAGAGGTAGAAGGTGTTGCGCGTGAAATTCGTAAACTTGTGGCGGAGGAAAACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTACAATATCCCACACTTCATCGATG
AAAAGCGTCCGATGTCACACCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGGTGGACTTCAGAGGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATCACAGATAGTGAACGTGAAATGGAAGAGAAAATAAATAGGTTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTCTA
CTTATTTCTAGAAGAGCTGGATGTTCCGAAAAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAGATGCTTGGTGAAGAGAAA
ATGTCTCTTTCTATGTTTACGGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGACGTGAAAGCAACGTTTATTATAGGTGTAAATGAGGGTG
TTATTCCAGCAGCACCTATGGACGAAGGTATGCTTTCTGATGAAGAAAGAGAAGTTCTTGGTGCAGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
TATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGACGTAAACGATTTATCACGTTCAGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAACTAAGTACAGCAGTAAGTAGAGATTTATACGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCATACGCTCATTTTGCGCAGCACGGTTTATCGCTTAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCGGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGCTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCCATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGATTCGTACCAGTTGATTTAGAAGTGCCGTTCGGTATGGGTGGTACAGGCTCACTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTTGTCGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGGGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCGCAGACGTGGTTGAAAAAAGGTCACGCAGCATCACCAGCTGGTG
TATTGTATTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ATGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.659

100

0.497

Multiple sequence alignment