Detailed information    

experimental Experimentally validated

Overview

Name   pilC/pilC2   Type   Machinery gene
Locus tag   QG077_RS00720 Genome accession   NZ_JARXTD010000002
Coordinates   12720..17228 (-) Length   1502 a.a.
NCBI ID   WP_285068138.1    Uniprot ID   B6SE68
Organism   Kingella kingae strain KK03     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The PilC1 and PilC2 C-terminal domains are not sufficient for twitching motility but support transformation.


Genomic Context

Location: 7720..22228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QG077_RS00710 (QG077_00710) - 8369..10486 (+) 2118 WP_285068139.1 M3 family metallopeptidase -
  QG077_RS00715 (QG077_00715) mnmG 10529..12415 (-) 1887 WP_019389758.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  QG077_RS00720 (QG077_00720) pilC/pilC2 12720..17228 (-) 4509 WP_285068138.1 pilus assembly protein Machinery gene
  QG077_RS00725 (QG077_00725) - 17440..18147 (-) 708 WP_285057840.1 SAM-dependent methyltransferase -
  QG077_RS00730 (QG077_00730) - 18277..19557 (+) 1281 WP_285068136.1 valine--pyruvate transaminase -
  QG077_RS00735 (QG077_00735) ccoG 19654..21081 (+) 1428 WP_019389761.1 cytochrome c oxidase accessory protein CcoG -
  QG077_RS00740 (QG077_00740) - 21085..21672 (+) 588 WP_019389762.1 FixH family protein -
  QG077_RS00745 (QG077_00745) - 21755..22228 (-) 474 WP_019389763.1 hemerythrin domain-containing protein -

Sequence

Protein


Download         Length: 1502 a.a.        Molecular weight: 164317.90 Da        Isoelectric Point: 7.8759

>NTDB_id=1190 QG077_RS00720 WP_285068138.1 12720..17228(-) (pilC/pilC2) [Kingella kingae strain KK03]
MKPASKKCISTQLRQLNKMLWVAFPLLSLPFYAQAENNTPFSDSSLTGIQKTYGPNVTLALSVEFPTAGAAYSTATEFTE
AMMSQTFLGYFDNTKCYEYVSPSDRNGMKAFAASGYKQNSGSDNVSKDNRGRLLLAPDGTQALAGKQRPWIFDYAGRRFS
VPDPAYSNNILYESIRQKEFFLHDSMDTSLNDSMGTSGAVNHKYETDEREYFHPTRTASTSNGMVGVCEGEKEFSGNFMN
WATMSAIDIFRQAMTGGNRALGVAKDTTAYVAGDTPTQTFLRRANVVRAQNAHYMQRTVRLSEANIRKVLPHDYAVDGVY
PTLANNGRESPSNNFFLYQGYVPHPYAYRMRLDSSRQWKEETRQTSIKAMTGKVTLATNRPLLVRNSGFGVDFRRAVHDV
SGNGWYHKAFRRDDWSSGSDWWSGATWLGDINDGTNGRYWAPVKSRVMPYQVTVEACVPDKLEANCVRQPSGSYKPEGLM
QQNASTMRFAAFGYANIAGNTVSGGVLRSRMRYIANPNKDGATATGPAVKYQEEINPKTGQFYVNPDRNAEETAAQAFVV
NTGDKNLPSSNFDNSGTINYLNKFGDYNNYKTNDPGAELYYTALRYLRNKGFPALYKTKLAEATRSNITREVRDNFPLIA
DNWDNPLNVFSDNTTLNVADNVCRPNYIIYIGDTNTHADASLPGGGNTQYDFNVNDDPDIHVRDLINEKISPNEPRLSWW
NGNWGADASPGGAMAALALWGRTNDLQQNFGKRLSSFMIDTVEDGKFKPENNNYWLAAKYGGFNDLNNNGVPDKGEWEAT
SASDKVSAFEAPAVSNGTPQNFAVANNPTSMVAALNRAFEATATAEAPSATSLGTTSTKPLSERGKTMLLQSVFRDATTV
VNGARVKVASGDVLGLEATLKGHKLEYEKKWSAGEKLQAAYHNANGWTNRKVFTRANANSGAVRLQGSNNAVTSVVGGSD
AADLVNYALGAPTYEDGRKFRIRPNHLMGTVINSPVVTIPSRTGSVSTVAGSCTYPASANISSRANRHVVAANDGMFYVL
NSSGQEIASYMPSTALSQLARYASPNYSHFFMNDGIAATSEVCFDTGNDKGNGKAHTVVVGTAGRGGNSVYALDLTNPTD
LTESDMLWEFTHDGLGKSLFAPIITHDSTGTPVAIVSSGYNAKEDNGYVYVLKLNKPVGSPWVEDTHPTKPWGNNGNWYR
IKLGAGGVGELFAYQNEARTVAAVYGGDLEGKLWKVSQNAEGRFVAGYVDSANNTLPIFKTADKTSIVGAPFAQIVGGKT
YVVFITGRYFNKSDLPSTTQTVQNYAYGIIESKISDNRNPTASGALIEDGENLLQQHVEEEIVPDNVRQTVFYKVTNHQI
TNKHQGWKLKLQKNWLSIDKSAIRGKRVAEFSAVNPLATDVASADNMCTENGSTYSLSVNVFNGGVYNKPIYDTNGDGKF
TEADTLVSVAGQAGILTRLTEVSTEFGRIVGGINSMGNMIQMPKDNITSDPVVKRVSWREIF

Nucleotide


Download         Length: 4509 bp        

>NTDB_id=1190 QG077_RS00720 WP_285068138.1 12720..17228(-) (pilC/pilC2) [Kingella kingae strain KK03]
ATGAAGCCAGCAAGCAAAAAGTGCATTTCAACGCAACTGCGCCAGCTCAATAAAATGCTGTGGGTAGCCTTTCCTTTGCT
ATCCTTGCCTTTTTATGCACAAGCAGAAAACAATACCCCTTTTTCTGATTCATCATTAACAGGTATTCAAAAAACCTACG
GCCCGAACGTAACATTGGCTTTGTCGGTGGAGTTTCCAACCGCAGGTGCGGCGTATTCTACAGCAACCGAATTTACCGAA
GCCATGATGAGCCAAACCTTTTTGGGCTATTTCGACAACACCAAATGCTATGAATACGTTTCCCCCAGTGACCGAAACGG
TATGAAGGCGTTTGCTGCTAGTGGCTATAAGCAAAATAGTGGCTCGGATAATGTATCCAAGGATAATAGAGGGCGACTGT
TGCTTGCGCCAGATGGTACGCAAGCGTTGGCGGGTAAACAGCGCCCATGGATATTCGATTATGCTGGACGCAGGTTCAGT
GTTCCGGATCCAGCATACAGCAACAATATACTTTATGAATCAATACGACAGAAAGAATTCTTCTTGCATGATAGTATGGA
TACCAGTTTGAATGATAGTATGGGTACCAGCGGTGCAGTTAATCATAAATATGAAACGGACGAGCGAGAATATTTCCATC
CAACACGAACCGCCAGTACAAGCAATGGCATGGTGGGTGTTTGTGAAGGAGAAAAAGAATTTAGTGGTAACTTCATGAAC
TGGGCAACCATGTCGGCGATTGATATTTTCCGTCAAGCGATGACAGGTGGTAACCGTGCGCTGGGTGTTGCAAAAGACAC
AACTGCTTATGTGGCAGGTGATACGCCAACGCAAACGTTCTTGCGCCGTGCCAATGTTGTGCGCGCACAAAATGCGCACT
ATATGCAGCGCACAGTTCGATTAAGTGAAGCGAATATCAGAAAAGTTTTGCCACATGATTATGCTGTGGACGGGGTATAT
CCTACTTTGGCGAATAATGGTAGGGAATCGCCAAGTAATAATTTCTTCTTGTATCAGGGTTATGTTCCACATCCATACGC
ATATCGTATGCGGTTGGATTCAAGCAGGCAGTGGAAAGAGGAAACTCGACAAACCAGCATTAAGGCAATGACAGGTAAAG
TTACATTGGCGACTAATCGCCCATTGCTTGTACGTAACTCTGGTTTTGGCGTGGACTTCCGCCGTGCTGTTCATGACGTA
TCAGGTAATGGTTGGTATCACAAAGCGTTTAGGCGGGATGATTGGAGTTCAGGCAGCGATTGGTGGAGTGGTGCTACTTG
GTTGGGTGACATCAACGATGGTACAAATGGTCGCTATTGGGCGCCTGTTAAATCGCGTGTAATGCCTTATCAAGTAACCG
TAGAAGCGTGTGTGCCAGACAAATTGGAAGCCAACTGCGTACGTCAGCCATCTGGTTCGTATAAGCCAGAGGGTTTGATG
CAACAAAACGCGTCAACCATGCGTTTTGCAGCATTTGGTTACGCCAATATTGCAGGTAATACCGTGAGTGGTGGTGTGTT
ACGTAGCCGTATGCGTTATATCGCCAATCCAAATAAAGATGGAGCAACGGCAACAGGACCAGCTGTAAAATACCAAGAAG
AGATTAACCCAAAAACAGGTCAGTTCTATGTGAACCCAGACCGCAATGCAGAAGAAACAGCAGCGCAGGCATTTGTGGTT
AATACAGGCGATAAAAACTTGCCATCTTCTAATTTTGACAATTCGGGCACGATTAATTATCTGAACAAGTTTGGTGATTA
TAACAATTATAAAACCAATGACCCTGGTGCAGAGTTGTATTACACCGCATTGCGCTATTTGCGTAATAAAGGTTTCCCAG
CGTTGTACAAAACCAAGTTAGCAGAAGCGACTCGTAGCAATATTACAAGAGAGGTTCGCGATAACTTCCCATTGATTGCC
GACAATTGGGATAACCCATTAAACGTGTTCAGCGACAACACTACTTTGAATGTAGCGGATAACGTGTGTCGTCCAAACTA
TATTATCTATATTGGTGATACCAATACCCACGCTGATGCTTCATTGCCTGGCGGTGGGAATACGCAATACGATTTCAATG
TGAATGACGACCCAGACATTCATGTGCGTGACTTGATTAACGAGAAAATCAGCCCAAACGAACCTAGGTTGAGTTGGTGG
AATGGCAACTGGGGTGCAGATGCATCACCAGGTGGTGCAATGGCGGCATTGGCGTTGTGGGGTCGAACCAACGATTTGCA
GCAGAATTTTGGTAAGCGTTTAAGTAGCTTTATGATTGATACGGTTGAGGATGGTAAATTTAAACCTGAAAATAATAACT
ATTGGTTGGCAGCCAAATACGGTGGCTTCAACGATTTAAATAATAATGGTGTACCTGATAAAGGCGAATGGGAAGCAACT
TCCGCTTCGGATAAAGTGTCGGCATTTGAAGCACCAGCGGTGTCTAATGGTACGCCACAGAACTTTGCGGTAGCCAACAA
CCCAACTTCAATGGTAGCAGCATTGAACCGTGCGTTTGAAGCCACAGCCACAGCCGAAGCACCATCTGCAACCAGCTTGG
GCACAACTAGCACCAAACCTTTGTCAGAACGCGGTAAAACTATGTTGTTGCAATCCGTGTTCCGCGACGCAACCACTGTG
GTAAATGGTGCGCGTGTTAAAGTGGCATCGGGCGATGTGTTGGGATTGGAAGCGACCTTAAAAGGTCATAAGTTGGAGTA
CGAGAAAAAATGGTCGGCTGGCGAAAAATTGCAGGCTGCTTATCACAATGCAAATGGTTGGACAAATCGTAAGGTGTTTA
CTCGTGCTAACGCCAATAGCGGTGCAGTTCGTTTGCAAGGTAGCAATAATGCAGTTACGAGCGTTGTAGGTGGCTCAGAT
GCAGCAGATTTGGTTAATTACGCTTTGGGTGCACCAACGTATGAAGATGGCAGAAAATTCCGTATTCGTCCAAACCATTT
GATGGGTACAGTGATTAACTCGCCAGTAGTAACCATTCCATCACGCACAGGCAGCGTTAGCACCGTGGCAGGTAGCTGTA
CTTATCCTGCATCTGCCAATATTAGCTCGCGTGCAAATCGCCATGTGGTAGCAGCCAATGATGGTATGTTCTATGTGTTG
AACTCGTCAGGTCAGGAAATTGCATCGTATATGCCATCAACTGCGTTGAGTCAGTTGGCACGTTATGCGTCGCCAAATTA
CAGCCATTTCTTTATGAATGACGGTATTGCCGCTACTTCTGAAGTATGTTTTGACACGGGTAATGACAAGGGTAATGGCA
AGGCGCATACGGTAGTAGTTGGCACAGCAGGTCGTGGTGGTAACAGCGTGTATGCGTTGGATTTGACCAACCCAACAGAC
TTGACTGAAAGTGATATGCTGTGGGAGTTTACGCACGACGGCTTGGGTAAGAGCTTGTTTGCGCCAATCATCACGCACGA
TAGCACTGGTACACCTGTGGCGATTGTGAGCAGTGGCTATAATGCAAAAGAAGACAATGGCTATGTGTATGTGCTGAAAT
TGAACAAACCTGTTGGCAGTCCTTGGGTTGAAGATACACACCCAACCAAACCATGGGGCAATAACGGTAACTGGTATCGC
ATTAAACTGGGTGCTGGTGGTGTAGGCGAATTGTTTGCTTACCAAAATGAAGCACGCACCGTTGCAGCTGTGTATGGTGG
CGATTTAGAAGGCAAGCTGTGGAAGGTATCGCAAAATGCTGAAGGTCGCTTTGTAGCGGGTTATGTGGATAGCGCAAATA
ACACGTTGCCTATCTTCAAAACAGCAGACAAGACGTCTATTGTGGGTGCGCCTTTTGCACAAATCGTAGGTGGTAAAACG
TATGTTGTGTTTATTACTGGTCGCTACTTCAACAAGAGCGATTTGCCTAGCACAACCCAAACAGTACAAAACTACGCATA
CGGCATTATCGAGAGCAAAATCAGCGATAACCGCAATCCAACTGCTAGCGGTGCATTGATTGAAGACGGTGAGAACTTGT
TGCAACAACATGTGGAAGAGGAAATTGTTCCAGACAACGTGCGACAAACCGTGTTCTATAAGGTTACGAATCACCAAATT
ACCAACAAGCATCAAGGTTGGAAGTTGAAACTGCAAAAAAACTGGCTGAGTATTGATAAGAGTGCGATTCGCGGCAAACG
TGTTGCAGAGTTTAGCGCAGTGAATCCGTTGGCGACCGATGTGGCTAGCGCCGATAATATGTGTACCGAAAACGGTTCGA
CTTATTCGTTGTCAGTTAATGTGTTCAATGGTGGTGTGTACAACAAACCTATCTATGACACAAATGGCGATGGTAAGTTT
ACCGAAGCGGATACTTTGGTATCTGTGGCTGGTCAGGCTGGTATTTTGACTCGCTTAACCGAAGTTTCTACTGAGTTCGG
TCGTATTGTTGGCGGTATCAACAGTATGGGTAACATGATTCAAATGCCAAAAGACAATATCACCAGCGACCCTGTGGTGA
AACGTGTATCTTGGCGCGAGATTTTCTAA

Domains

Predicted by InterproScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(988-1341)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Alexandra L Sacharok et al. (2022) Kingella kingae PilC1 and PilC2 are adhesive multifunctional proteins that promote bacterial adherence, twitching motility, DNA transformation, and pilus biogenesis. PLoS Pathogens 18(3):e1010440. [PMID: 35353876]