Detailed information    

experimental Experimentally validated

Overview

Name   pilC/pilC1   Type   Machinery gene
Locus tag   QG077_RS09545 Genome accession   NZ_JARXTD010000096
Coordinates   293..4372 (+) Length   1359 a.a.
NCBI ID   WP_019389114.1    Uniprot ID   -
Organism   Kingella kingae strain KK03     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The PilC1 and PilC2 C-terminal domains are not sufficient for twitching motility but support transformation.


Genomic Context

Location: 1..9372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QG077_RS09545 (QG077_09545) pilC/pilC1 293..4372 (+) 4080 WP_019389114.1 PilC/PilY family type IV pilus protein Machinery gene

Sequence

Protein


Download         Length: 1359 a.a.        Molecular weight: 147598.28 Da        Isoelectric Point: 8.0352

>NTDB_id=1189 QG077_RS09545 WP_019389114.1 293..4372(+) (pilC/pilC1) [Kingella kingae strain KK03]
MSLKYKKYNCNLKPLSLGVSLALSSFATMAADSPFAIAPQHLSSTATVKKTTVTTPYADIFKQQQSITNTTVRGMQGAKP
NIMLLLDDSGSMGAQVPGSGRTRQQILQSSLSQVVAKYGNQINWGLIAFNDQSSAFNLPLGTNYSTVVNSIKRFPANGLT
PTITSYIKAVDTLNKGIQYRCQKSYLVMLSDGDSNYPMYFEREAGVNARPGQMLTSYLRYPHWVYINREINKYDFWRELL
TKDGKLIDRNSSKYRGMCSTAYQKSNSNVKNWCSALPQQFVYYPYSGISDPDDTTTSAYRINYMNTWSGIFAKKYTEYPL
RDLTNDLDFLDSGSAYYTNTNLPSSGFGRYKKFFFNNQPILAPQTQYAGAMLSFFSEPINKIDLRIGGNDATGKSWDDSA
FPTQNITTFTIGFGNGLSESGLALLRGGASNIKLKDGTVQKAYYSASDQTALQKAFDAILAQVEAENPPPSNSNPIDLPP
TTNTLPISAPTAGVPNRQQSVMAETSYATSPPSATGASNSIPNMAASVFVPAGLKSSELRFYALNSKAQADTSSWLVADF
SQRKALIRTSSGVRWANDANLDNTFFLLSADTQTPQRTDEWNKALLPWTTRTVEGSDDSKIKALNYPTPYRIRPIPPTDS
PRSTGVDEYNMGDVLDTPIVATGKNGIAQGNRQEFLITAANDGMVYLFQSNPDVNAKNPYSLKLNYLPATMPRQTLTDTH
ATYYKDLAHEDYAKDSARPHLFMINGGITVRTTSAKSNRQNTFMVGALGQGGRGMYALNIGGKALDSGNAIGLNAEDSTW
NTSVPLYESNPNNSTDFSSLGYTVGYPQIGRIAKYNTDGSLSDNSLKTGVYYASFLSSGFSSLNASQETALYIQDALGVD
VGGGANTAATANGNTKGKLLAKVVVPGGVGGLASPTLLDVNRDGVYDFVFAGDYGGNMYRFDLRKIDLSNGTVTDGAVKK
IYSGSTTQPITSAPAISMQKNGRYVVAFGTGSDMYASDYNSTAEQAVYGIYQRFNERLDPIDINNPTAAETITPLTAGNL
LEQTLTTATAKMAEGTSNEKEVTVREGSTNAIESPSDKSKLAYGGWKVRLGNTTSVQDGERVVVTPTVIFGTLILSTRIY
KEKPVATVNNNAWSENNWQADGWVKVREDLDQNRTESPWSGWKEISTNSTSTTSSGDICTGTVNTSTKMQERERKVNYET
ITTYEKVQPSENQSSGWILVLNATNGGAVKVSSGTGVDFLGKYDASNFNPNDTAHSNTYFTVGLYSSSGLPNHTLLTNDS
NVHSLGSAMTRDGEARTNGEDGDLTPGGEKAKDDCFNNTDGNYVLTGNTATGIGATFSVVGRRCGKKIQIRRLSWREIF

Nucleotide


Download         Length: 4080 bp        

>NTDB_id=1189 QG077_RS09545 WP_019389114.1 293..4372(+) (pilC/pilC1) [Kingella kingae strain KK03]
ATGAGTCTTAAATATAAAAAATATAATTGCAATCTTAAACCATTAAGCTTAGGGGTATCACTTGCCTTGTCCAGCTTTGC
GACAATGGCGGCTGATTCACCATTTGCAATTGCACCACAACATTTATCCAGTACGGCAACTGTAAAAAAAACCACAGTAA
CGACACCGTATGCCGATATTTTCAAACAGCAACAATCAATTACAAATACAACAGTGCGTGGTATGCAGGGTGCTAAACCC
AATATTATGCTACTATTGGATGACTCAGGAAGTATGGGTGCTCAAGTTCCAGGCTCTGGTCGAACACGACAACAAATATT
GCAAAGTTCCTTATCACAAGTAGTTGCTAAGTATGGTAATCAAATTAACTGGGGGCTTATTGCATTTAATGATCAAAGTT
CCGCATTTAACCTTCCATTAGGGACAAATTATTCAACCGTTGTTAATTCAATTAAAAGATTTCCTGCTAATGGTCTGACA
CCAACTATTACCAGCTACATAAAGGCTGTAGATACACTAAATAAAGGTATTCAATACCGTTGCCAAAAAAGTTATTTAGT
TATGTTATCTGATGGCGATTCTAACTATCCTATGTATTTTGAGCGAGAAGCAGGAGTTAATGCTAGACCTGGTCAGATGC
TTACTAGCTATTTAAGATATCCACACTGGGTTTATATTAACAGAGAAATTAATAAATACGATTTTTGGCGTGAACTCTTA
ACAAAAGATGGTAAATTAATTGATAGAAATTCATCCAAATATCGTGGAATGTGCAGTACTGCTTATCAAAAATCTAATTC
GAATGTTAAAAACTGGTGCAGTGCCTTACCTCAACAATTTGTTTATTATCCATATAGTGGTATTTCTGACCCTGATGATA
CTACCACCTCTGCTTATCGAATAAATTATATGAATACTTGGTCAGGTATTTTCGCAAAAAAATATACCGAATACCCATTG
CGGGATTTAACTAATGATTTAGATTTTTTGGATAGTGGTTCTGCATACTATACAAATACAAACCTACCTTCATCAGGATT
TGGAAGATATAAAAAATTTTTCTTCAACAATCAACCTATTTTGGCTCCCCAGACCCAATATGCGGGCGCGATGTTGTCAT
TTTTTAGCGAGCCAATCAACAAAATTGACCTACGAATAGGTGGTAATGATGCTACAGGTAAATCATGGGATGACTCAGCT
TTTCCAACACAAAACATTACAACCTTTACTATTGGTTTTGGTAATGGTCTGAGTGAGAGTGGTTTGGCATTATTGCGCGG
TGGTGCCAGCAATATTAAATTAAAAGATGGAACAGTACAAAAAGCGTACTATTCCGCTAGCGATCAAACAGCATTGCAAA
AGGCATTTGATGCTATTTTGGCGCAAGTTGAAGCGGAAAATCCACCACCTAGCAATAGTAATCCTATTGATTTGCCTCCC
ACTACTAATACCTTGCCAATTTCTGCACCTACGGCAGGTGTACCTAATCGCCAGCAAAGTGTGATGGCGGAGACGTCTTA
TGCCACTTCTCCTCCTAGTGCAACAGGTGCTAGCAATAGTATTCCTAATATGGCGGCATCGGTGTTTGTCCCTGCAGGGC
TAAAAAGTAGCGAATTGCGTTTTTATGCTTTAAATAGCAAAGCGCAAGCAGATACTTCGTCTTGGCTTGTGGCTGACTTT
TCGCAACGTAAAGCGTTAATCAGGACAAGTTCGGGTGTACGATGGGCAAATGATGCAAACTTGGATAATACGTTCTTTTT
ATTGTCTGCTGATACGCAAACGCCTCAACGGACTGATGAGTGGAATAAGGCATTATTGCCTTGGACAACTCGTACTGTTG
AAGGCAGCGATGACAGCAAAATTAAAGCGTTGAATTACCCAACGCCTTATCGTATTCGTCCAATTCCACCTACTGATAGT
CCACGTAGTACTGGCGTTGATGAATATAATATGGGCGATGTGTTGGATACACCGATTGTTGCGACTGGTAAAAATGGCAT
TGCGCAAGGTAATCGACAAGAGTTTTTGATTACAGCGGCCAATGATGGCATGGTGTACTTGTTCCAAAGTAATCCTGATG
TCAATGCAAAAAATCCGTATTCATTAAAATTGAATTACTTACCTGCGACAATGCCGCGCCAAACATTGACTGATACTCAT
GCTACTTATTACAAAGATTTAGCACACGAGGATTATGCAAAAGATTCTGCGCGTCCACATTTATTTATGATTAATGGTGG
TATTACGGTTCGTACGACTTCCGCTAAATCTAATCGTCAAAACACGTTTATGGTGGGGGCTTTGGGACAAGGCGGACGTG
GTATGTACGCACTCAATATTGGCGGTAAGGCTTTGGATTCTGGTAACGCCATCGGCTTAAATGCCGAAGATAGTACATGG
AATACATCCGTGCCTTTATATGAATCTAATCCAAATAATAGTACAGATTTTTCTTCATTAGGCTATACCGTTGGTTATCC
ACAAATTGGTCGTATTGCTAAATATAACACTGATGGCTCTTTATCAGACAATAGTTTGAAAACAGGTGTTTATTATGCTT
CTTTCTTATCAAGTGGCTTTTCTTCTCTTAATGCTAGTCAGGAAACCGCTCTGTATATTCAAGATGCGTTGGGTGTAGAT
GTTGGTGGTGGTGCTAATACTGCTGCTACAGCAAATGGCAATACAAAAGGTAAATTATTAGCGAAAGTGGTGGTTCCAGG
CGGCGTAGGTGGTTTGGCATCGCCAACATTACTTGATGTGAATCGAGATGGTGTGTATGACTTTGTCTTTGCGGGAGATT
ATGGTGGCAATATGTATCGCTTTGATTTGCGTAAAATTGATTTGTCTAATGGTACTGTTACCGACGGCGCTGTGAAGAAA
ATTTATTCTGGTTCTACAACACAACCAATTACAAGTGCTCCTGCTATTTCCATGCAAAAAAATGGACGGTATGTTGTGGC
ATTTGGTACGGGTAGTGATATGTATGCTAGCGATTATAATTCAACCGCTGAGCAAGCTGTATATGGTATTTATCAACGAT
TCAACGAGCGTTTGGATCCTATTGATATCAATAATCCTACTGCTGCTGAAACTATAACCCCTTTAACAGCTGGTAATTTG
TTGGAGCAAACATTAACCACAGCGACTGCCAAAATGGCAGAGGGTACTTCTAACGAAAAAGAAGTAACTGTTCGTGAAGG
CTCAACCAATGCAATTGAAAGTCCTAGTGATAAATCAAAATTAGCCTATGGTGGTTGGAAAGTGCGATTAGGTAACACGA
CATCTGTACAAGATGGTGAACGTGTTGTGGTTACCCCAACTGTGATTTTTGGTACCTTGATTCTTTCAACTCGCATCTAT
AAAGAAAAACCAGTTGCTACGGTCAATAATAATGCTTGGTCAGAAAATAATTGGCAAGCTGATGGTTGGGTTAAAGTAAG
GGAAGATTTAGACCAAAATCGCACAGAATCTCCATGGTCTGGTTGGAAAGAAATCAGCACGAACTCAACATCAACCACTA
GTTCAGGTGATATTTGTACGGGTACTGTGAATACCAGCACTAAAATGCAAGAGCGCGAAAGAAAGGTTAATTATGAAACC
ATTACCACTTATGAGAAAGTACAGCCAAGTGAGAATCAATCATCTGGATGGATTTTAGTATTGAATGCGACTAATGGCGG
TGCGGTAAAAGTATCATCTGGTACTGGAGTTGACTTTTTAGGTAAGTATGATGCTAGTAACTTTAATCCTAATGATACAG
CCCATAGTAATACCTATTTTACCGTAGGTTTGTACTCTTCCAGCGGTTTACCAAATCATACATTATTAACCAATGATTCT
AATGTTCATAGTTTAGGTTCTGCGATGACTCGTGATGGGGAAGCAAGAACCAATGGTGAAGATGGTGATTTAACTCCTGG
TGGTGAAAAGGCAAAGGATGATTGCTTTAATAATACTGACGGTAACTATGTATTGACAGGTAATACGGCAACAGGAATTG
GCGCAACATTTAGCGTTGTTGGTCGTCGTTGTGGTAAAAAAATACAAATCCGTCGTTTATCTTGGCGTGAGATTTTCTAA

Domains

Predicted by InterproScan.

von Willebrand factor type A domain (VWA_2)

(82-192)

Neisseria PilC beta-propeller domain (T4P_PilY1)

(667-1048)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Alexandra L Sacharok et al. (2022) Kingella kingae PilC1 and PilC2 are adhesive multifunctional proteins that promote bacterial adherence, twitching motility, DNA transformation, and pilus biogenesis. PLoS Pathogens 18(3):e1010440. [PMID: 35353876]