Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG62_RS19115 Genome accession   NZ_CP010219
Coordinates   3669440..3669880 (-) Length   146 a.a.
NCBI ID   WP_000360927.1    Uniprot ID   -
Organism   Escherichia coli strain M18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3664440..3674880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG62_RS19095 (RG62_18165) coaE 3664930..3665550 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  RG62_RS28445 - 3665575..3665619 (+) 45 WP_120795372.1 protein YacM -
  RG62_RS19100 (RG62_18170) guaC 3665775..3666818 (+) 1044 WP_001217338.1 GMP reductase -
  RG62_RS19105 (RG62_18175) hofC 3666853..3668055 (-) 1203 WP_000157236.1 protein transport protein HofC -
  RG62_RS19110 (RG62_18180) pilB 3668045..3669430 (-) 1386 WP_001025170.1 type II secretion system protein GspE Machinery gene
  RG62_RS19115 (RG62_18185) pilA 3669440..3669880 (-) 441 WP_000360927.1 prepilin peptidase-dependent pilin Machinery gene
  RG62_RS19125 (RG62_18195) nadC 3670083..3670976 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG62_RS19130 (RG62_18200) ampD 3671064..3671615 (+) 552 WP_000923716.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG62_RS19135 (RG62_18205) ampE 3671612..3672466 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RG62_RS19140 (RG62_18210) aroP 3672509..3673882 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15533.60 Da        Isoelectric Point: 4.2529

>NTDB_id=118074 RG62_RS19115 WP_000360927.1 3669440..3669880(-) (pilA) [Escherichia coli strain M18]
MDKQSGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=118074 RG62_RS19115 WP_000360927.1 3669440..3669880(-) (pilA) [Escherichia coli strain M18]
ATGGACAAGCAAAGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

42.742

84.932

0.363

Multiple sequence alignment