Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG62_RS12345 Genome accession   NZ_CP010219
Coordinates   2342698..2343165 (+) Length   155 a.a.
NCBI ID   WP_000340167.1    Uniprot ID   A7ZSL1
Organism   Escherichia coli strain M18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2337698..2348165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG62_RS12295 (RG62_11640) rplP 2337808..2338218 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  RG62_RS12300 (RG62_11645) rpsC 2338231..2338932 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  RG62_RS12305 (RG62_11650) rplV 2338950..2339282 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  RG62_RS12310 (RG62_11655) rpsS 2339297..2339575 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  RG62_RS12315 (RG62_11660) rplB 2339592..2340413 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  RG62_RS12320 (RG62_11665) rplW 2340431..2340733 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  RG62_RS12325 (RG62_11670) rplD 2340730..2341335 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  RG62_RS12330 (RG62_11675) rplC 2341346..2341975 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  RG62_RS12335 (RG62_11680) rpsJ 2342008..2342319 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  RG62_RS12345 (RG62_11690) pilD 2342698..2343165 (+) 468 WP_000340167.1 A24 family peptidase Machinery gene
  RG62_RS12350 (RG62_11695) bfr 2343162..2343638 (-) 477 WP_000675515.1 bacterioferritin -
  RG62_RS12355 (RG62_11700) bfd 2343711..2343905 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RG62_RS12365 (RG62_11705) tuf 2344088..2345272 (-) 1185 WP_000031783.1 elongation factor Tu -
  RG62_RS12370 (RG62_11710) fusA 2345343..2347457 (-) 2115 WP_000124700.1 elongation factor G -
  RG62_RS12375 (RG62_11715) rpsG 2347554..2348024 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16984.17 Da        Isoelectric Point: 8.0293

>NTDB_id=118043 RG62_RS12345 WP_000340167.1 2342698..2343165(+) (pilD) [Escherichia coli strain M18]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDCLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=118043 RG62_RS12345 WP_000340167.1 2342698..2343165(+) (pilD) [Escherichia coli strain M18]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACTGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZSL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361

Multiple sequence alignment