Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG57_RS10015 Genome accession   NZ_CP010191
Coordinates   1960264..1960731 (-) Length   155 a.a.
NCBI ID   WP_000340167.1    Uniprot ID   A7ZSL1
Organism   Escherichia coli strain M8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1955264..1965731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG57_RS09985 (RG57_09465) rpsG 1955405..1955875 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  RG57_RS09990 (RG57_09470) fusA 1955972..1958086 (+) 2115 WP_000124700.1 elongation factor G -
  RG57_RS09995 (RG57_09475) tuf 1958157..1959341 (+) 1185 WP_000031783.1 elongation factor Tu -
  RG57_RS10005 (RG57_09480) bfd 1959524..1959718 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RG57_RS10010 (RG57_09485) bfr 1959791..1960267 (+) 477 WP_000675515.1 bacterioferritin -
  RG57_RS10015 (RG57_09490) pilD 1960264..1960731 (-) 468 WP_000340167.1 A24 family peptidase Machinery gene
  RG57_RS10025 (RG57_09500) rpsJ 1961110..1961421 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  RG57_RS10030 (RG57_09505) rplC 1961454..1962083 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  RG57_RS10035 (RG57_09510) rplD 1962094..1962699 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  RG57_RS10040 (RG57_09515) rplW 1962696..1962998 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  RG57_RS10045 (RG57_09520) rplB 1963016..1963837 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  RG57_RS10050 (RG57_09525) rpsS 1963854..1964132 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  RG57_RS10055 (RG57_09530) rplV 1964147..1964479 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  RG57_RS10060 (RG57_09535) rpsC 1964497..1965198 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  RG57_RS10065 (RG57_09540) rplP 1965211..1965621 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16984.17 Da        Isoelectric Point: 8.0293

>NTDB_id=117640 RG57_RS10015 WP_000340167.1 1960264..1960731(-) (pilD) [Escherichia coli strain M8]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDCLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=117640 RG57_RS10015 WP_000340167.1 1960264..1960731(-) (pilD) [Escherichia coli strain M8]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACTGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZSL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361

Multiple sequence alignment