Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   ACLV65_RS01665 Genome accession   NZ_OZ217342
Coordinates   306944..307768 (+) Length   274 a.a.
NCBI ID   WP_411864366.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis B22     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 301944..312768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV65_RS01635 (SMIB22_03160) rsmD 303091..303630 (+) 540 WP_411864362.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACLV65_RS01640 (SMIB22_03170) coaD 303620..304108 (+) 489 WP_411864363.1 pantetheine-phosphate adenylyltransferase -
  ACLV65_RS01645 (SMIB22_03180) sepM 304092..305129 (+) 1038 WP_411864364.1 SepM family pheromone-processing serine protease Regulator
  ACLV65_RS01650 (SMIB22_03190) - 305187..305417 (+) 231 WP_138706243.1 DUF1146 family protein -
  ACLV65_RS01655 (SMIB22_03200) murA 305441..306724 (+) 1284 WP_411864365.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ACLV65_RS01660 (SMIB22_03210) - 306717..306905 (+) 189 WP_055386865.1 DNA-directed RNA polymerase subunit beta -
  ACLV65_RS01665 (SMIB22_03220) endA 306944..307768 (+) 825 WP_411864366.1 DNA/RNA non-specific endonuclease Machinery gene
  ACLV65_RS01670 (SMIB22_03230) - 307923..309254 (+) 1332 WP_411864367.1 hemolysin family protein -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29859.43 Da        Isoelectric Point: 9.9431

>NTDB_id=1170097 ACLV65_RS01665 WP_411864366.1 306944..307768(+) (endA) [Streptococcus mitis isolate S. mitis B22]
MNKKTRQTLIGLLVLLLLSAGSYYIKQNELGNQAAKTKMSQKSQSPDAPGQELAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLNAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESKVRKALDKNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=1170097 ACLV65_RS01665 WP_411864366.1 306944..307768(+) (endA) [Streptococcus mitis isolate S. mitis B22]
ATGAACAAAAAAACAAGACAGACACTGATTGGTTTGCTAGTGTTATTGCTCTTATCTGCGGGGAGCTATTATATTAAGCA
GAATGAACTAGGCAATCAGGCTGCTAAGACAAAAATGAGTCAGAAAAGTCAATCACCAGACGCGCCTGGTCAAGAATTGG
CGGAAAGTGTCTTAACAGATGCAGTCAAAAGCCAAATAAAAGGGAGTCTGGAGTGGAACGGATCAGGTGCTTTTATCGTC
AATGGCAATAAAACAAACCTAAATGCCAAGGTTTCAAGTAAGCCCTACGCTGATAATAAGACAAAGACAGTGGGCAAGGA
AACAGTGCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCTACTCGCCAGTACAAGAATCGTGAAGAAACCGGGAATGGTT
CGACTTCTTGGACTCCACCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCGCAGGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGTAAGGTGCGTAAAGCCTTGGATA
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

92.336

100

0.923
  endA Streptococcus pneumoniae D39

92.336

100

0.923
  endA Streptococcus pneumoniae R6

92.336

100

0.923
  endA Streptococcus pneumoniae TIGR4

92.336

100

0.923

Multiple sequence alignment