Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AB3213_RS06640 Genome accession   NZ_OZ061411
Coordinates   1298086..1298598 (-) Length   170 a.a.
NCBI ID   WP_035145013.1    Uniprot ID   A0A9N7J0G1
Organism   Latilactobacillus sakei isolate Latilactobacillus sakei CIRM-BIA1912     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1293086..1303598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3213_RS06610 - 1294012..1294377 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  AB3213_RS06620 - 1294920..1295378 (-) 459 WP_011374992.1 hypothetical protein -
  AB3213_RS06625 - 1295433..1296629 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  AB3213_RS06630 - 1296651..1297661 (-) 1011 WP_099947695.1 class I SAM-dependent methyltransferase -
  AB3213_RS06635 - 1297785..1298051 (-) 267 WP_016265373.1 hypothetical protein -
  AB3213_RS06640 comGF 1298086..1298598 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  AB3213_RS06645 comGE 1298573..1298878 (-) 306 WP_016265375.1 hypothetical protein Machinery gene
  AB3213_RS06650 comGD 1298865..1299317 (-) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  AB3213_RS06655 comGC 1299289..1299588 (-) 300 WP_011374999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AB3213_RS06660 comGB 1299585..1300592 (-) 1008 WP_011375000.1 type II secretion system F family protein Machinery gene
  AB3213_RS06665 comGA 1300585..1301475 (-) 891 WP_016265378.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3213_RS06670 - 1301590..1302321 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AB3213_RS06675 - 1302412..1302912 (-) 501 WP_099947696.1 VanZ family protein -
  AB3213_RS06680 - 1302991..1303383 (+) 393 WP_231952369.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19689.58 Da        Isoelectric Point: 6.9899

>NTDB_id=1166142 AB3213_RS06640 WP_035145013.1 1298086..1298598(-) (comGF) [Latilactobacillus sakei isolate Latilactobacillus sakei CIRM-BIA1912]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLNGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=1166142 AB3213_RS06640 WP_035145013.1 1298086..1298598(-) (comGF) [Latilactobacillus sakei isolate Latilactobacillus sakei CIRM-BIA1912]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTCACGCAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCGGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGCCAATTAAATGGGTATATGCCATTATTAGGCAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.235

100

0.982

Multiple sequence alignment