Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACE4RF_RS04240 Genome accession   NZ_OZ059817
Coordinates   807361..808431 (+) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus isolate E6_22S00629-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 802361..813431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE4RF_RS04210 - 802784..804247 (+) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -
  ACE4RF_RS04215 - 804228..804431 (+) 204 WP_000087559.1 YqgQ family protein -
  ACE4RF_RS04220 - 804428..805414 (+) 987 WP_000161315.1 ROK family glucokinase -
  ACE4RF_RS04225 - 805414..805743 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACE4RF_RS04230 - 805740..806363 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACE4RF_RS04235 comGA 806415..807389 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACE4RF_RS04240 comGB 807361..808431 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACE4RF_RS04245 comGC 808445..808756 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACE4RF_RS04250 comGD 808734..809180 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACE4RF_RS04255 comGE 809167..809466 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACE4RF_RS04260 comGF 809384..809881 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACE4RF_RS04265 - 810072..811243 (+) 1172 Protein_798 IS256-like element IS256 family transposase -
  ACE4RF_RS04270 - 811310..811456 (+) 147 WP_001789879.1 hypothetical protein -
  ACE4RF_RS04275 - 811446..811970 (+) 525 WP_001015120.1 shikimate kinase -
  ACE4RF_RS04280 gcvT 812129..813220 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=1165075 ACE4RF_RS04240 WP_000776425.1 807361..808431(+) (comGB) [Staphylococcus aureus isolate E6_22S00629-1]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1165075 ACE4RF_RS04240 WP_000776425.1 807361..808431(+) (comGB) [Staphylococcus aureus isolate E6_22S00629-1]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment