Detailed information    

experimental Experimentally validated

Overview

Name   recX   Type   Regulator
Locus tag   BSU_08520 Genome accession   NC_000964
Coordinates   925633..926427 (+) Length   264 a.a.
NCBI ID   NP_388733.1    Uniprot ID   O31575
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   inhibit excessive RecA-mediated recombination   
Competence regulation

Function

RecX is a regulatory protein that modulates the activity of RecA, a key enzyme in homologous recombination. RecX directly binds to RecA filaments, inhibiting excessive RecA-mediated recombination and strand exchange during natural transformation. This ensures controlled and accurate integration of exogenous DNA into the genome.


Genomic Context

Location: 920633..931427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_08460 (BSU08460) sxzA 921472..922503 (+) 1032 NP_388727.1 xenosiderophore schizokinen (dihydroxamate) transporter (permease) -
  BSU_08470 (BSU08470) yfhB 922618..923499 (+) 882 NP_388728.1 putative isomerase -
  BSU_08480 (BSU08480) yfhC 923587..924171 (+) 585 NP_388729.1 putative oxidoreductase (nitroreductase family) -
  BSU_08490 (BSU08490) yfhD 924210..924401 (-) 192 NP_388730.1 hypothetical protein -
  BSU_08500 (BSU08500) yfhE 924468..924578 (-) 111 NP_388731.1 hypothetical protein -
  BSU_08510 (BSU08510) yfhF 924633..925544 (-) 912 NP_388732.1 putative nucleotide-or NAD-binding protein -
  BSU_08520 (BSU08520) recX 925633..926427 (+) 795 NP_388733.1 regulatory protein RecX Regulator
  BSU_08530 (BSU08530) yfhH 926429..926743 (+) 315 NP_388734.1 hypothetical protein -
  BSU_08540 (BSU08540) yfhI 926886..928079 (+) 1194 NP_388735.1 putative efflux transporter -
  BSU_08550 (BSU08550) sspK 928112..928264 (-) 153 YP_054573.1 small acid-soluble spore protein -
  BSU_08560 (BSU08560) yfhJ 928389..928658 (+) 270 NP_388736.1 hypothetical protein -
  BSU_08570 (BSU08570) yfhK 928803..929321 (+) 519 NP_388737.1 hypothetical protein -
  BSU_08580 (BSU08580) spdL 929406..929738 (+) 333 NP_388738.1 factor for peptide SdpC immunity -
  BSU_08590 (BSU08590) ephM 929725..930585 (+) 861 NP_388739.1 stress induced epoxide hydrolase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  recX recA negative effect

Sequence

Protein


Download         Length: 264 a.a.        Molecular weight: 31013.23 Da        Isoelectric Point: 6.6686

>NTDB_id=116 BSU_08520 NP_388733.1 925633..926427(+) (recX) [Bacillus subtilis subsp. subtilis str. 168]
MPFITKISTQKKNTERFNIFLDDKYAFSVDADVLVKFELKKGKELDDLDIIEIQYGDEVKKGFNRALDFLSYRMRSTKEV
EDHLKKKETSPPVIAEVIHRLNDYKYLNDQEFAAAYVSTHKKTNGKGPDVLFRELRAKGIDDDTIKEALSSFSFEDQTRE
AVKHVEKLLKKDKKLSTKELKQRAQLQLQRKGFSFDVISAAMDQIEYENDEDTEKEALRLHAEKAFRKYRYDGSYESAMK
VKQFLFRKGFSLDLIEQLLQEEEY

Nucleotide


Download         Length: 795 bp        

>NTDB_id=116 BSU_08520 NP_388733.1 925633..926427(+) (recX) [Bacillus subtilis subsp. subtilis str. 168]
ATGCCGTTTATTACAAAAATATCAACGCAAAAAAAGAATACGGAACGCTTCAATATTTTTCTGGATGATAAATACGCCTT
CAGCGTGGACGCGGATGTGCTCGTGAAATTTGAGCTCAAGAAGGGAAAAGAACTCGATGATCTTGACATCATTGAGATTC
AATACGGCGATGAAGTGAAGAAAGGCTTCAACCGTGCGCTTGACTTTTTATCCTATCGAATGCGGTCGACGAAAGAGGTC
GAGGATCACTTAAAGAAAAAAGAAACCTCGCCCCCCGTCATTGCGGAAGTCATCCATAGGCTTAACGATTACAAGTACTT
AAACGACCAGGAATTTGCCGCAGCCTATGTCAGCACTCACAAAAAAACAAACGGAAAAGGCCCTGATGTTTTGTTCAGAG
AGCTAAGAGCGAAGGGAATTGATGACGATACAATTAAGGAGGCGCTAAGTTCCTTTTCCTTTGAAGATCAGACAAGAGAA
GCGGTCAAGCATGTTGAAAAGCTTCTCAAAAAAGATAAAAAGCTTTCGACGAAAGAACTCAAACAGCGCGCCCAGCTGCA
GCTTCAGCGCAAGGGCTTCTCATTTGATGTTATCAGCGCGGCGATGGATCAGATTGAATATGAAAATGATGAAGACACAG
AGAAAGAAGCGCTGCGCCTTCACGCGGAAAAAGCGTTTAGAAAATACCGCTATGACGGCTCATATGAAAGCGCCATGAAG
GTCAAACAATTTTTATTCCGCAAAGGATTCTCACTCGATTTAATCGAGCAATTACTGCAGGAAGAGGAGTACTGA

Domains

Predicted by InterproScan.

RecX family (RecX_HTH2)

(84-204)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O31575

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Ester Serrano et al. (2021) Recombination proteins differently control the acquisition of homeologous DNA during Bacillus subtilis natural chromosomal transformation. Environmental Microbiology 23(1):512-524. [PMID: 33264457]
[2] Shimin Le et al. (2017) Bacillus subtilis RecA with DprA-SsbA antagonizes RecX function during natural transformation. Nucleic Acids Research 45(15):8873-8885. [PMID: 28911099]
[3] Ignacija Vlašić et al. (2014) Bacillus subtilis RecA and its accessory factors, RecF, RecO, RecR and RecX, are required for spore resistance to DNA double-strand break. Nucleic Acids Research 42(4):2295-307. [PMID: 24285298]
[4] Paula P Cárdenas et al. (2012) RecX facilitates homologous recombination by modulating RecA activities. PLoS Genetics 8(12):e1003126. [PMID: 23284295]