Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   QWI70_RS01135 Genome accession   NZ_OX465025
Coordinates   190749..191723 (+) Length   324 a.a.
NCBI ID   WP_001008574.1    Uniprot ID   A0AAV3JGK6
Organism   Streptococcus agalactiae isolate MRI Z2-329     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 190964..241534 190749..191723 flank -759


Gene organization within MGE regions


Location: 190749..241534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWI70_RS01135 comYH 190749..191723 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  QWI70_RS01140 - 191755..192948 (+) 1194 WP_000047534.1 acetate kinase -
  QWI70_RS01145 - 193100..193306 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  QWI70_RS01150 - 193365..193502 (+) 138 WP_001865900.1 hypothetical protein -
  QWI70_RS01155 - 193543..193998 (+) 456 WP_000905673.1 hypothetical protein -
  QWI70_RS01160 - 194067..194732 (+) 666 WP_000008113.1 type II CAAX endopeptidase family protein -
  QWI70_RS01165 proC 194753..195523 (-) 771 WP_001865901.1 pyrroline-5-carboxylate reductase -
  QWI70_RS01170 pepA 195593..196660 (-) 1068 WP_001281323.1 glutamyl aminopeptidase -
  QWI70_RS01175 - 196845..197084 (-) 240 WP_000660180.1 hypothetical protein -
  QWI70_RS01180 - 197245..197529 (+) 285 WP_000791272.1 DUF4651 domain-containing protein -
  QWI70_RS01185 - 197526..197849 (+) 324 WP_000602781.1 thioredoxin family protein -
  QWI70_RS01190 ytpR 197882..198508 (+) 627 WP_000578328.1 YtpR family tRNA-binding protein -
  QWI70_RS01195 - 198562..199278 (-) 717 WP_000186185.1 class I SAM-dependent methyltransferase -
  QWI70_RS01200 ssbA 199359..199754 (+) 396 WP_000282447.1 single-stranded DNA-binding protein Machinery gene
  QWI70_RS01205 - 199878..200522 (+) 645 WP_000416612.1 HAD family hydrolase -
  QWI70_RS01210 - 200549..202294 (+) 1746 WP_000930334.1 LytS/YhcK type 5TM receptor domain-containing protein -
  QWI70_RS01215 - 202275..203015 (+) 741 WP_000697630.1 LytTR family transcriptional regulator DNA-binding domain-containing protein -
  QWI70_RS01220 - 203185..203640 (+) 456 WP_000683316.1 CidA/LrgA family protein -
  QWI70_RS01225 lrgB 203642..204370 (+) 729 WP_000421726.1 antiholin-like protein LrgB -
  QWI70_RS01230 - 204613..206241 (+) 1629 WP_000170504.1 ABC transporter substrate-binding protein -
  QWI70_RS01235 - 206354..207331 (+) 978 WP_000680645.1 ABC transporter permease -
  QWI70_RS01240 - 207328..208149 (+) 822 WP_000603397.1 ABC transporter permease -
  QWI70_RS01245 - 208161..208964 (+) 804 WP_000140984.1 ABC transporter ATP-binding protein -
  QWI70_RS01250 - 208948..209574 (+) 627 WP_000171311.1 ABC transporter ATP-binding protein -
  QWI70_RS01255 treP 209857..211887 (+) 2031 WP_000434616.1 PTS system trehalose-specific EIIBC component -
  QWI70_RS01260 treC 212109..213734 (+) 1626 WP_000151017.1 alpha,alpha-phosphotrehalase -
  QWI70_RS01265 - 213950..215986 (+) 2037 WP_000228180.1 BglG family transcription antiterminator -
  QWI70_RS01270 - 215989..216273 (+) 285 WP_000944235.1 PTS sugar transporter subunit IIB -
  QWI70_RS01275 - 216286..217641 (+) 1356 WP_000677351.1 PTS ascorbate transporter subunit IIC -
  QWI70_RS01280 - 217644..218501 (+) 858 WP_000203489.1 transketolase -
  QWI70_RS01285 - 218498..219427 (+) 930 WP_001203821.1 transketolase C-terminal domain-containing protein -
  QWI70_RS01290 - 219536..220795 (+) 1260 WP_001203071.1 ferric reductase-like transmembrane domain-containing protein -
  QWI70_RS01295 rpsO 220883..221152 (+) 270 WP_001018249.1 30S ribosomal protein S15 -
  QWI70_RS01300 pnp 221533..223662 (+) 2130 WP_000043850.1 polyribonucleotide nucleotidyltransferase -
  QWI70_RS01305 - 223664..224416 (+) 753 WP_000204782.1 SseB family protein -
  QWI70_RS01310 cysE 224425..225009 (+) 585 WP_000539954.1 serine O-acetyltransferase -
  QWI70_RS01315 - 225019..225201 (+) 183 WP_000656476.1 lipoprotein -
  QWI70_RS01320 cysS 225198..226541 (+) 1344 WP_000591125.1 cysteine--tRNA ligase -
  QWI70_RS01325 - 226534..226920 (+) 387 WP_000568029.1 Mini-ribonuclease 3 -
  QWI70_RS01330 rlmB 227023..227778 (+) 756 WP_000178026.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QWI70_RS01335 - 227775..228293 (+) 519 WP_000716636.1 NYN domain-containing protein -
  QWI70_RS01340 - 228386..229246 (+) 861 WP_000143135.1 DegV family protein -
  QWI70_RS01345 - 229783..229902 (+) 120 Protein_209 helix-turn-helix domain-containing protein -
  QWI70_RS01350 rplM 230127..230573 (+) 447 WP_001865567.1 50S ribosomal protein L13 -
  QWI70_RS01355 rpsI 230594..230986 (+) 393 WP_000035940.1 30S ribosomal protein S9 -
  QWI70_RS01360 - 231114..232268 (-) 1155 WP_000022164.1 site-specific integrase -
  QWI70_RS01365 - 232332..232922 (-) 591 WP_000181098.1 helix-turn-helix transcriptional regulator -
  QWI70_RS01370 - 233077..233382 (+) 306 WP_000331954.1 hypothetical protein -
  QWI70_RS01375 - 233518..233796 (+) 279 WP_000134670.1 hypothetical protein -
  QWI70_RS01380 - 233807..234037 (+) 231 WP_000360142.1 hypothetical protein -
  QWI70_RS01385 - 234050..234376 (+) 327 WP_000384270.1 replication initiator protein A -
  QWI70_RS01390 - 234380..235009 (+) 630 WP_000591148.1 hypothetical protein -
  QWI70_RS01395 - 235333..236209 (+) 877 Protein_219 replication initiation protein -
  QWI70_RS01400 - 236245..236679 (+) 435 WP_001220479.1 hypothetical protein -
  QWI70_RS01405 mobV 236995..238251 (+) 1257 WP_000122832.1 MobV family relaxase -
  QWI70_RS01410 - 238419..238889 (+) 471 WP_000130119.1 hypothetical protein -
  QWI70_RS01415 - 239194..239529 (-) 336 WP_000384858.1 type II toxin-antitoxin system RelE/ParE family toxin -
  QWI70_RS01420 - 239519..239806 (-) 288 WP_000255538.1 hypothetical protein -
  QWI70_RS01425 - 240209..241429 (-) 1221 WP_000156558.1 site-specific integrase -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 37046.02 Da        Isoelectric Point: 4.5224

>NTDB_id=1159845 QWI70_RS01135 WP_001008574.1 190749..191723(+) (comYH) [Streptococcus agalactiae isolate MRI Z2-329]
MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSCDLDMVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQT
EQLQANHQFTPDSIGFILLFLLEELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
AQFIQEDAVRPQILKESDVIISDLPVGYYPNDGIAKRYAVSSSKEHTYAHHLLMEQSLKYLKKDGIAIFLAPENLLTSPQ
SDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFVLKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSK
NMIK

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1159845 QWI70_RS01135 WP_001008574.1 190749..191723(+) (comYH) [Streptococcus agalactiae isolate MRI Z2-329]
ATGAATTTTGAAAAAATTGAGACAGCCTATGAGCTGATTTTAGAAAATATCCAAACGATTGAGAACCAATTAAAAACTCA
TATTTATGATGCCTTAATTGAACAGAACTCTTATTACCTTGGTTCAAGTTGTGATTTAGATATGGTTGTGGTGAATAACC
AAAAATTACGTCAACTTGACTTAAGTCAAGAAGAATGGCGTCGCACTTTCCAGTTCATTTTTATCAAATCTGCGCAAACA
GAGCAATTACAAGCTAATCATCAGTTTACGCCAGATAGTATTGGTTTTATCTTGTTATTTCTTTTGGAAGAATTAACGAG
TCAAGAGACAGTGGATGTCTTGGAAATTGGAAGTGGAACTGGGAATTTAGCTCAGACTCTCCTCAATAACAGCTCGAAAG
AGTTAAATTATATGGGCATTGAAGTTGATGATCTTTTGATTGATCTATCAGCAAGCATTGCTGAAATTATAGGTTCTAGT
GCCCAATTTATCCAAGAGGATGCTGTTAGACCACAAATTTTGAAAGAAAGCGATGTAATCATTAGTGATTTACCAGTTGG
CTATTATCCTAATGATGGTATTGCTAAACGATATGCTGTATCAAGTTCTAAAGAGCACACCTATGCTCACCATCTATTGA
TGGAGCAATCTCTTAAATATTTGAAAAAAGATGGAATCGCTATATTTTTAGCACCCGAAAACCTTTTAACAAGTCCACAA
AGTGATTTGCTGAAGGAGTGGTTAAAAGGATATGCAGATGTCATTGCCGTTTTAACTCTACCAGAAACTATTTTTGGAAG
TCGTCAAAATGCGAAATCTATATTTGTTCTCAAGAAGCAAGCAGAACAAAAACCAGAAACCTTTGTATATCCGCTGACAG
ATTTGCAAAATCGTGAGAATATGGCAAACTTCATTGAAAATTTTCAAAAATGGAGCAGAGAAAATAGTCATTACTCAAAA
AATATGATAAAATAG

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(70-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

67.302

97.222

0.654
  comYH Streptococcus mutans UA140

67.302

97.222

0.654

Multiple sequence alignment