Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   KJP43_RS05720 Genome accession   NZ_OX419579
Coordinates   1104766..1108266 (+) Length   1166 a.a.
NCBI ID   WP_003233101.1    Uniprot ID   A0A8I1WCP0
Organism   Bacillus subtilis isolate NRS6103     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1099766..1113266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP43_RS05700 (NRS6103_05625) yhjO 1100625..1101830 (-) 1206 WP_003233111.1 MFS transporter -
  KJP43_RS05705 (NRS6103_05630) yhjP 1101944..1103671 (+) 1728 WP_003245281.1 SgrR family transcriptional regulator -
  KJP43_RS05710 (NRS6103_05635) yhjQ 1103701..1104027 (-) 327 WP_009966950.1 four-helix bundle copper-binding protein -
  KJP43_RS05715 (NRS6103_05640) yhjR 1104145..1104582 (-) 438 WP_003245806.1 ferritin family protein -
  KJP43_RS05720 (NRS6103_05645) addB 1104766..1108266 (+) 3501 WP_003233101.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  KJP43_RS05725 (NRS6103_05650) addA 1108253..1111951 (+) 3699 WP_003233100.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  KJP43_RS05730 (NRS6103_05655) sbcD 1112023..1113198 (+) 1176 WP_003233099.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134613.65 Da        Isoelectric Point: 5.5235

>NTDB_id=1157883 KJP43_RS05720 WP_003233101.1 1104766..1108266(+) (addB) [Bacillus subtilis isolate NRS6103]
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTKTPELAHLEAQYEARPAIPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEDDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSMIVKRLEELFPHHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=1157883 KJP43_RS05720 WP_003233101.1 1104766..1108266(+) (addB) [Bacillus subtilis isolate NRS6103]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCAACAGCATTCAGGATGAATTGCG
CCGGGCTCCATTCGGGAAGCCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGTTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGCTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTGTTTTATCTGAA
AAGCTCCATGACTTATCAATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATATAAAAGGCGCCCATATCTATGTGGATGGCTTTTATCAGTTTA
CCCCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCGGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTGGACATTACGTATAAAGAGCTGAGCGGGACTGAGCGGCATACAAAGACGCCGGAATTGGCGCATCTAG
AGGCACAGTATGAAGCGCGTCCGGCCATTCCATACGCAGAAAAACAAGAAGCCCTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCTCGGGAAATTCACGCCCTAGTCAGAGAGAAGGGATATCGCTATAAGGATGTAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATTGAATTTATCCGGTCGAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTCAATGAACCGAAGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCCTATGGTATTAAAGGCGACCGCTGGACAAAGGGCGATCGGTTCCAATACAGGCGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACTGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGCATGAAAAAAGCGAAGACGGTTCAAGAGAAGGCGGAGGCGCTCTATCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGATGACGGCAGAATTATTGAAG
CGCAGCAACATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGCACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTCCTGTCGGATGACGACCGGGAATGGCTGAAAACGATTGGGGTTGAGCTATCC
TCAGGCGGACGAGAGCGTTTGCTTGATGAGCACTTCCTCATCTACATGGCGTTTTCAAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCTGATGCAGAAGGAAAAACGCTTTTGCCGTCGATGATCGTTAAGCGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTAACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTGAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACACGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAGTCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCA
TGCCCGTTTTCCCATTTTGCGTCACACGGGCTGCATTTGAAGGAACGGCAATTCTTCAAGCTTGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCTGATAGGCTGCGTGAGCAAAAGCTGGATTGGCGCGATTTAACGAAGG
AGCAGTGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTCTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGTGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAACTCGTCGGGCGAATTGACCGTGTTGATAAGGCTGAAAGCTCAAAAGGCCTGCTCCTCAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGATTGGCACTGCAAATGCTGACGTACCTTGA
TTTATCGATTACACATTCAGCTGACTGGCTCGGGATGAGGGCGACGCCTGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCAGACTCAGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GCACCTTCCAAGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCACTGGAAGAAAACGAGTATCGCCCATTAAAGGCTGA
AAAGGACAAGACAATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(305-668)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

99.914

100

0.999

Multiple sequence alignment