Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG32_RS02235 Genome accession   NZ_CP010132
Coordinates   399826..400293 (+) Length   155 a.a.
NCBI ID   WP_040090373.1    Uniprot ID   -
Organism   Escherichia coli strain C10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394826..405293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG32_RS02185 (RG32_02075) rplP 394936..395346 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  RG32_RS02190 (RG32_02080) rpsC 395359..396060 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  RG32_RS02195 (RG32_02085) rplV 396078..396410 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  RG32_RS02200 (RG32_02090) rpsS 396425..396703 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  RG32_RS02205 (RG32_02095) rplB 396720..397541 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  RG32_RS02210 (RG32_02100) rplW 397559..397861 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  RG32_RS02215 (RG32_02105) rplD 397858..398463 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  RG32_RS02220 (RG32_02110) rplC 398474..399103 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  RG32_RS02225 (RG32_02115) rpsJ 399136..399447 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  RG32_RS02235 (RG32_02125) pilD 399826..400293 (+) 468 WP_040090373.1 A24 family peptidase Machinery gene
  RG32_RS02240 (RG32_02130) bfr 400290..400766 (-) 477 WP_000675515.1 bacterioferritin -
  RG32_RS02245 (RG32_02135) bfd 400839..401033 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RG32_RS02255 (RG32_02140) tuf 401216..402400 (-) 1185 WP_000031783.1 elongation factor Tu -
  RG32_RS02260 (RG32_02145) fusA 402471..404585 (-) 2115 WP_000124700.1 elongation factor G -
  RG32_RS02265 (RG32_02150) rpsG 404682..405152 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16922.08 Da        Isoelectric Point: 8.1338

>NTDB_id=115648 RG32_RS02235 WP_040090373.1 399826..400293(+) (pilD) [Escherichia coli strain C10]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGFRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=115648 RG32_RS02235 WP_040090373.1 399826..400293(+) (pilD) [Escherichia coli strain C10]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTTTCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.6

80.645

0.368