Detailed information NTG1150159

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: DG247_RS01340
• Genome accession: NZ_LT992486
• Coordinates: 271061..272077 (+)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae strain 4295STDY6534216
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 266061..277077

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DG247_RS01325 (SAMEA4374366_00253)	-	266603..267334 (-)	732	WP_000495223.1	glutathione peroxidase	-
DG247_RS01330 (SAMEA4374366_00254)	oxyR	267474..268367 (+)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
DG247_RS01335 (SAMEA4374366_00255)	-	268441..270918 (-)	2478	WP_000668733.1	penicillin-binding protein 1A	-
DG247_RS01340 (SAMEA4374366_00256)	pilM	271061..272077 (+)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
DG247_RS01345 (SAMEA4374366_00257)	pilN	272067..272651 (+)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
DG247_RS01350 (SAMEA4374366_00258)	pilO	272644..273234 (+)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
DG247_RS01355 (SAMEA4374366_00259)	pilP	273224..273742 (+)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
DG247_RS01360 (SAMEA4374366_00260)	pilQ	273768..275483 (+)	1716	WP_000788426.1	type IV pilus secretin PilQ	Machinery gene
DG247_RS01365 (SAMEA4374366_00261)	aroK	275670..276194 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=1150159 DG247_RS01340 WP_000520444.1 271061..272077(+) (pilM) [Vibrio cholerae strain 4295STDY6534216]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=1150159 DG247_RS01340 WP_000520444.1 271061..272077(+) (pilM) [Vibrio cholerae strain 4295STDY6534216]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485